Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant
Auxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this st...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Jing Chen Shijie Wang Fengling Wu Min Wei Jing Li Fengjuan Yang |
| author_facet | Jing Chen Shijie Wang Fengling Wu Min Wei Jing Li Fengjuan Yang |
| author_sort | Jing Chen |
| collection | DOAJ |
| description | Auxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this study, genome–wide identification and the functional analysis of the ARF gene family in eggplants (<i>Solanum melongena</i> L.) were performed. A total of 20 <i>ARF</i> (<i>SmARF</i>) genes were identified and phylogenetically classified into three groups. Our analysis revealed four functional domains and 10 motifs in these proteins. Subcellular localization showed that the SmARFs localized in the nucleus. To investigate the biological functions of the <i>SmARFs</i> under 2,4–D and salt stress treatments, quantitative real–time RT–PCR (qRT–PCR) was conducted. Most <i>SmARF</i> genes exhibited changes in expression in response to 2,4–D treatments in the flowers, especially <i>SmARF4</i> and <i>7B</i>. All <i>SmARF</i> genes quickly responded to salt stress, except <i>SmARF17</i> and <i>19</i> in leaves, <i>SmARF1A</i> and <i>7B</i> in roots, and <i>SmARF2A</i>, <i>SmARF7B</i>, and <i>SmARF16B</i> in stems. These results helped to elucidate the role of ARFs in auxin signaling under 2,4–D and salt stress in eggplants. |
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| issn | 1661-6596 1422-0067 |
| language | English |
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| publishDate | 2022-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-acd6d163fcd2419398b6950723e263fa2023-11-23T14:11:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012311621910.3390/ijms23116219Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in EggplantJing Chen0Shijie Wang1Fengling Wu2Min Wei3Jing Li4Fengjuan Yang5State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaAuxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this study, genome–wide identification and the functional analysis of the ARF gene family in eggplants (<i>Solanum melongena</i> L.) were performed. A total of 20 <i>ARF</i> (<i>SmARF</i>) genes were identified and phylogenetically classified into three groups. Our analysis revealed four functional domains and 10 motifs in these proteins. Subcellular localization showed that the SmARFs localized in the nucleus. To investigate the biological functions of the <i>SmARFs</i> under 2,4–D and salt stress treatments, quantitative real–time RT–PCR (qRT–PCR) was conducted. Most <i>SmARF</i> genes exhibited changes in expression in response to 2,4–D treatments in the flowers, especially <i>SmARF4</i> and <i>7B</i>. All <i>SmARF</i> genes quickly responded to salt stress, except <i>SmARF17</i> and <i>19</i> in leaves, <i>SmARF1A</i> and <i>7B</i> in roots, and <i>SmARF2A</i>, <i>SmARF7B</i>, and <i>SmARF16B</i> in stems. These results helped to elucidate the role of ARFs in auxin signaling under 2,4–D and salt stress in eggplants.https://www.mdpi.com/1422-0067/23/11/6219genome–wideauxin response factor geneseggplantauxin signaling2,4–Dsalt stress |
| spellingShingle | Jing Chen Shijie Wang Fengling Wu Min Wei Jing Li Fengjuan Yang Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant International Journal of Molecular Sciences genome–wide auxin response factor genes eggplant auxin signaling 2,4–D salt stress |
| title | Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant |
| title_full | Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant |
| title_fullStr | Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant |
| title_full_unstemmed | Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant |
| title_short | Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant |
| title_sort | genome wide identification and functional characterization of auxin response factor arf genes in eggplant |
| topic | genome–wide auxin response factor genes eggplant auxin signaling 2,4–D salt stress |
| url | https://www.mdpi.com/1422-0067/23/11/6219 |
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