Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection
Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/14/2354 |
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| author | Jimmy Jiun-Ming Su Chih-Hsin Lin Hsuan Chen Shyh-Yuan Lee Yuan-Min Lin |
| author_facet | Jimmy Jiun-Ming Su Chih-Hsin Lin Hsuan Chen Shyh-Yuan Lee Yuan-Min Lin |
| author_sort | Jimmy Jiun-Ming Su |
| collection | DOAJ |
| description | Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration. |
| first_indexed | 2024-03-10T09:26:52Z |
| format | Article |
| id | doaj.art-acd8436ce249487c986c7dc8c97c37d4 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T09:26:52Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-acd8436ce249487c986c7dc8c97c37d42023-11-22T04:47:01ZengMDPI AGPolymers2073-43602021-07-011314235410.3390/polym13142354Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light ProjectionJimmy Jiun-Ming Su0Chih-Hsin Lin1Hsuan Chen2Shyh-Yuan Lee3Yuan-Min Lin4Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanGraduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, TaiwanDepartment of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, TaiwanGelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration.https://www.mdpi.com/2073-4360/13/14/2354gelatin methacrylatehydroxyapatitesilanizationmesenchymal stem cellsdigital light processing projection3D printing |
| spellingShingle | Jimmy Jiun-Ming Su Chih-Hsin Lin Hsuan Chen Shyh-Yuan Lee Yuan-Min Lin Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection Polymers gelatin methacrylate hydroxyapatite silanization mesenchymal stem cells digital light processing projection 3D printing |
| title | Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection |
| title_full | Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection |
| title_fullStr | Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection |
| title_full_unstemmed | Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection |
| title_short | Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection |
| title_sort | biofabrication of cell laden gelatin methacryloyl hydrogels with incorporation of silanized hydroxyapatite by visible light projection |
| topic | gelatin methacrylate hydroxyapatite silanization mesenchymal stem cells digital light processing projection 3D printing |
| url | https://www.mdpi.com/2073-4360/13/14/2354 |
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