Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment
More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based resea...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2023-08-01
|
| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/29/8/22-1818_article |
| _version_ | 1797775171680993280 |
|---|---|
| author | Egie E. Enabulele Winka Le Clec’h Emma K. Roberts Cody W. Thompson Molly M. McDonough Adam W. Ferguson Robert D. Bradley Timothy J. C. Anderson Roy N. Platt |
| author_facet | Egie E. Enabulele Winka Le Clec’h Emma K. Roberts Cody W. Thompson Molly M. McDonough Adam W. Ferguson Robert D. Bradley Timothy J. C. Anderson Roy N. Platt |
| author_sort | Egie E. Enabulele |
| collection | DOAJ |
| description |
More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues.
|
| first_indexed | 2024-03-12T22:31:53Z |
| format | Article |
| id | doaj.art-acd864c5ed8946bcabd159d8b11caa46 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-03-12T22:31:53Z |
| publishDate | 2023-08-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-acd864c5ed8946bcabd159d8b11caa462023-07-21T14:58:50ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592023-08-012981566157910.3201/eid2908.221818Prospecting for Zoonotic Pathogens by Using Targeted DNA EnrichmentEgie E. EnabuleleWinka Le Clec’hEmma K. RobertsCody W. ThompsonMolly M. McDonoughAdam W. FergusonRobert D. BradleyTimothy J. C. AndersonRoy N. Platt More than 60 zoonoses are linked to small mammals, including some of the most devastating pathogens in human history. Millions of museum-archived tissues are available to understand natural history of those pathogens. Our goal was to maximize the value of museum collections for pathogen-based research by using targeted sequence capture. We generated a probe panel that includes 39,916 80-bp RNA probes targeting 32 pathogen groups, including bacteria, helminths, fungi, and protozoans. Laboratory-generated, mock-control samples showed that we are capable of enriching targeted loci from pathogen DNA 2,882‒6,746-fold. We identified bacterial species in museum-archived samples, including Bartonella, a known human zoonosis. These results showed that probe-based enrichment of pathogens is a highly customizable and efficient method for identifying pathogens from museum-archived tissues. https://wwwnc.cdc.gov/eid/article/29/8/22-1818_articleprospectingzoonotic pathogenstargeted DNA enrichmentbacteriagenetic archivenatural history museum |
| spellingShingle | Egie E. Enabulele Winka Le Clec’h Emma K. Roberts Cody W. Thompson Molly M. McDonough Adam W. Ferguson Robert D. Bradley Timothy J. C. Anderson Roy N. Platt Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment Emerging Infectious Diseases prospecting zoonotic pathogens targeted DNA enrichment bacteria genetic archive natural history museum |
| title | Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment |
| title_full | Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment |
| title_fullStr | Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment |
| title_full_unstemmed | Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment |
| title_short | Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment |
| title_sort | prospecting for zoonotic pathogens by using targeted dna enrichment |
| topic | prospecting zoonotic pathogens targeted DNA enrichment bacteria genetic archive natural history museum |
| url | https://wwwnc.cdc.gov/eid/article/29/8/22-1818_article |
| work_keys_str_mv | AT egieeenabulele prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT winkaleclech prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT emmakroberts prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT codywthompson prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT mollymmcdonough prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT adamwferguson prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT robertdbradley prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT timothyjcanderson prospectingforzoonoticpathogensbyusingtargeteddnaenrichment AT roynplatt prospectingforzoonoticpathogensbyusingtargeteddnaenrichment |