Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry
A method combining SPE and Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) was developed for the simultaneous determination of nine polypeptide antibiotics in animal tissues. The samples were extracted by methanol and 0.1 mol/L hydrochloric acid (7:3, v/v), then cl...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-09-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120013 |
| _version_ | 1798017600012156928 |
|---|---|
| author | Lianwei LI Xiaocong YANG Minna YAO Zhengcai LIU Xiaojing LI |
| author_facet | Lianwei LI Xiaocong YANG Minna YAO Zhengcai LIU Xiaojing LI |
| author_sort | Lianwei LI |
| collection | DOAJ |
| description | A method combining SPE and Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) was developed for the simultaneous determination of nine polypeptide antibiotics in animal tissues. The samples were extracted by methanol and 0.1 mol/L hydrochloric acid (7:3, v/v), then cleaned with Alumina-A and n-hexane, and finally purified by hydrophilic-lipophilic balance (hydrophilic-lipophilic balance, HLB) cartridge. The separations were performed on a C8 column (100A, 150 mm×2 mm, 3 µm) using a mobile phase gradient with 0.2% formic acid and acetonitrile. Electrospray positive ionization and multi-reaction monitoring (MRM) mode were used for detection. The calibration curves for vancomycin, norvancomycin, enramycin A, enramycin B, and teicoplanin, (concentration range 2~200 μg/L), colistin A, colistin B and bacitracin A (concentration range 5~500 μg/L), virginiamycin M1 (concentration range 0.1~20 μg/L) were linear with correlation coefficients more than 0.99. The limits of quantification (LOQ, S/N=10) of 9 kinds of polypeptide antibiotics were varied from 1 µg/kg to 20 µg/kg, the detection limit of the method was 0.3 µg/kg to 6 µg/kg. The recovery experiments were performed with chicken, pork, liver and kidney samples spiked in the range of 1~200 μg/kg. The average recoveries ranged from 74.2% and 96.3% with the relative standard deviations (RSDs) of 4.3%~14.6% (n=6). The method is simple and rapid with high sensitivity and good reproducibility. Furthermore, this method is effective for the safety monitoring of polypeptide antibiotic residues in animal tissues. |
| first_indexed | 2024-04-11T16:09:54Z |
| format | Article |
| id | doaj.art-acdb1bca394f4aae9f25793357a282e7 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:09:54Z |
| publishDate | 2022-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-acdb1bca394f4aae9f25793357a282e72022-12-22T04:14:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-09-01431733033710.13386/j.issn1002-0306.20211200132021120013-17Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass SpectrometryLianwei LI0Xiaocong YANG1Minna YAO2Zhengcai LIU3Xiaojing LI4Food Science College of Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFood College of Xinyang College of Agriculture and Forestry, Xinyang 464000, ChinaFood Science College of Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaTechnology Center of Fuzhou Customs District, Fuzhou 350001, ChinaTechnology Center of Fuzhou Customs District, Fuzhou 350001, ChinaA method combining SPE and Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) was developed for the simultaneous determination of nine polypeptide antibiotics in animal tissues. The samples were extracted by methanol and 0.1 mol/L hydrochloric acid (7:3, v/v), then cleaned with Alumina-A and n-hexane, and finally purified by hydrophilic-lipophilic balance (hydrophilic-lipophilic balance, HLB) cartridge. The separations were performed on a C8 column (100A, 150 mm×2 mm, 3 µm) using a mobile phase gradient with 0.2% formic acid and acetonitrile. Electrospray positive ionization and multi-reaction monitoring (MRM) mode were used for detection. The calibration curves for vancomycin, norvancomycin, enramycin A, enramycin B, and teicoplanin, (concentration range 2~200 μg/L), colistin A, colistin B and bacitracin A (concentration range 5~500 μg/L), virginiamycin M1 (concentration range 0.1~20 μg/L) were linear with correlation coefficients more than 0.99. The limits of quantification (LOQ, S/N=10) of 9 kinds of polypeptide antibiotics were varied from 1 µg/kg to 20 µg/kg, the detection limit of the method was 0.3 µg/kg to 6 µg/kg. The recovery experiments were performed with chicken, pork, liver and kidney samples spiked in the range of 1~200 μg/kg. The average recoveries ranged from 74.2% and 96.3% with the relative standard deviations (RSDs) of 4.3%~14.6% (n=6). The method is simple and rapid with high sensitivity and good reproducibility. Furthermore, this method is effective for the safety monitoring of polypeptide antibiotic residues in animal tissues.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120013polypeptide antibioticanimal tissuesolid-phase extractionultra-high performance liquid chromatography tandem mass spectrometry (uplc-ms/ms) |
| spellingShingle | Lianwei LI Xiaocong YANG Minna YAO Zhengcai LIU Xiaojing LI Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry Shipin gongye ke-ji polypeptide antibiotic animal tissue solid-phase extraction ultra-high performance liquid chromatography tandem mass spectrometry (uplc-ms/ms) |
| title | Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_full | Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_fullStr | Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_full_unstemmed | Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_short | Determination of Nine Polypeptide Antibiotics Residues in Animal Tissue Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_sort | determination of nine polypeptide antibiotics residues in animal tissue food by ultra high performance liquid chromatography tandem mass spectrometry |
| topic | polypeptide antibiotic animal tissue solid-phase extraction ultra-high performance liquid chromatography tandem mass spectrometry (uplc-ms/ms) |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120013 |
| work_keys_str_mv | AT lianweili determinationofninepolypeptideantibioticsresiduesinanimaltissuefoodbyultrahighperformanceliquidchromatographytandemmassspectrometry AT xiaocongyang determinationofninepolypeptideantibioticsresiduesinanimaltissuefoodbyultrahighperformanceliquidchromatographytandemmassspectrometry AT minnayao determinationofninepolypeptideantibioticsresiduesinanimaltissuefoodbyultrahighperformanceliquidchromatographytandemmassspectrometry AT zhengcailiu determinationofninepolypeptideantibioticsresiduesinanimaltissuefoodbyultrahighperformanceliquidchromatographytandemmassspectrometry AT xiaojingli determinationofninepolypeptideantibioticsresiduesinanimaltissuefoodbyultrahighperformanceliquidchromatographytandemmassspectrometry |