Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by base paring with the complementary sequences of the target mRNAs, and then exert their function through degrading mRNA or inhibiting protein translation. They play a significant role as a regulatory factor in biological pr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253120301827 |
| _version_ | 1818931035806629888 |
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| author | Yingzhuang Song Zhijing Xu Fu Wang |
| author_facet | Yingzhuang Song Zhijing Xu Fu Wang |
| author_sort | Yingzhuang Song |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by base paring with the complementary sequences of the target mRNAs, and then exert their function through degrading mRNA or inhibiting protein translation. They play a significant role as a regulatory factor in biological processes of organism development, cell proliferation, differentiation, and cell death. Some of the traditional methods for studying miRNAs, such as northern blot, real-time PCR, or microarray, have been extensively used to investigate the biological properties and expression patterns of miRNAs. However, these methods often require considerable time, cell samples, and the design of effective primers or specific probes. Therefore, in order to gain a deeper understanding of the role of miRNAs in biological processes and accelerate the clinical application of miRNAs in the field of disease treatment, non-invasive, sensitive, and efficient imaging methods are needed to visualize the dynamic expression of miRNAs in living cells and animals. In this study, we reviewed the recent progress in the genetically encoded reporter genes for miRNA imaging. |
| first_indexed | 2024-12-20T04:10:12Z |
| format | Article |
| id | doaj.art-acdc57eea9d04a849805f5cc6f5538bd |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-20T04:10:12Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-acdc57eea9d04a849805f5cc6f5538bd2022-12-21T19:53:56ZengElsevierMolecular Therapy: Nucleic Acids2162-25312020-09-0121555567Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and AnimalsYingzhuang Song0Zhijing Xu1Fu Wang2Institute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaInstitute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaInstitute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University, Xi’an, Shaanxi 710071, China; Corresponding author: Fu Wang, Institute of Medical Research, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by base paring with the complementary sequences of the target mRNAs, and then exert their function through degrading mRNA or inhibiting protein translation. They play a significant role as a regulatory factor in biological processes of organism development, cell proliferation, differentiation, and cell death. Some of the traditional methods for studying miRNAs, such as northern blot, real-time PCR, or microarray, have been extensively used to investigate the biological properties and expression patterns of miRNAs. However, these methods often require considerable time, cell samples, and the design of effective primers or specific probes. Therefore, in order to gain a deeper understanding of the role of miRNAs in biological processes and accelerate the clinical application of miRNAs in the field of disease treatment, non-invasive, sensitive, and efficient imaging methods are needed to visualize the dynamic expression of miRNAs in living cells and animals. In this study, we reviewed the recent progress in the genetically encoded reporter genes for miRNA imaging.http://www.sciencedirect.com/science/article/pii/S2162253120301827miRNAimagingreporter gene |
| spellingShingle | Yingzhuang Song Zhijing Xu Fu Wang Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals Molecular Therapy: Nucleic Acids miRNA imaging reporter gene |
| title | Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals |
| title_full | Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals |
| title_fullStr | Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals |
| title_full_unstemmed | Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals |
| title_short | Genetically Encoded Reporter Genes for MicroRNA Imaging in Living Cells and Animals |
| title_sort | genetically encoded reporter genes for microrna imaging in living cells and animals |
| topic | miRNA imaging reporter gene |
| url | http://www.sciencedirect.com/science/article/pii/S2162253120301827 |
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