Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging
Glyceryl monooleate (GMO) is a polar lipid that can exist in various liquid crystalline phases in the presence of different amounts of water. It is regarded as a permeation enhancer due to its amphiphilic property. Various phases of GMO/solvent system containing sodium fluorescein were prepared to c...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-12-01
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| Series: | Asian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1818087614000683 |
| _version_ | 1811306444482412544 |
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| author | Dae Gon Lim Won-Wook Jeong Nam Ah Kim Jun Yeul Lim Seol-Hoon Lee Woo Sun Shim Nae-Gyu Kang Seong Hoon Jeong |
| author_facet | Dae Gon Lim Won-Wook Jeong Nam Ah Kim Jun Yeul Lim Seol-Hoon Lee Woo Sun Shim Nae-Gyu Kang Seong Hoon Jeong |
| author_sort | Dae Gon Lim |
| collection | DOAJ |
| description | Glyceryl monooleate (GMO) is a polar lipid that can exist in various liquid crystalline phases in the presence of different amounts of water. It is regarded as a permeation enhancer due to its amphiphilic property. Various phases of GMO/solvent system containing sodium fluorescein were prepared to compare permeability using confocal laser scanning microscopy (CLSM). GMO was melted in a vial in a water bath heated to 45 °C. Propylene glycol and hexanediol were homogeneously dissolved in the melted GMO. Sodium fluorescein in aqueous solution was diluted to various ratios and thoroughly mixed by an ultrasonic homogenizer. Each GMO/Solvent system with fluorescein was applied onto the epidermal side of excised pig skin and incubated overnight. CLSM was performed to observe how the GMO/solvent system in its different phases affect skin permeability. Cubic and lamellar phase formulations enhanced the fluorescein permeation through the stratum corneum. A solution system had the weakest permeability compared to the other two phases. Due to the amphiphilic nature of GMO, cubic and lamellar phases might reduce the barrier function of stratum corneum which was observed by CLSM as fluorescein accumulated in the dermis. Based on the results, the glyceryl monooleate lyotropic mixtures could be applied to enhance skin permeation in various topical and transdermal formulations. |
| first_indexed | 2024-04-13T08:44:33Z |
| format | Article |
| id | doaj.art-acde11252bda4de9aa61910426786ef2 |
| institution | Directory Open Access Journal |
| issn | 1818-0876 |
| language | English |
| last_indexed | 2024-04-13T08:44:33Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-acde11252bda4de9aa61910426786ef22022-12-22T02:53:45ZengElsevierAsian Journal of Pharmaceutical Sciences1818-08762014-12-019632432910.1016/j.ajps.2014.06.008Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imagingDae Gon Lim0Won-Wook Jeong1Nam Ah Kim2Jun Yeul Lim3Seol-Hoon Lee4Woo Sun Shim5Nae-Gyu Kang6Seong Hoon Jeong7College of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi 410-820, Republic of KoreaLG Household & Health Care, Daejeon 305-343, Republic of KoreaCollege of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi 410-820, Republic of KoreaCollege of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi 410-820, Republic of KoreaLG Household & Health Care, Daejeon 305-343, Republic of KoreaLG Household & Health Care, Daejeon 305-343, Republic of KoreaLG Household & Health Care, Daejeon 305-343, Republic of KoreaCollege of Pharmacy, Dongguk University-Seoul, Goyang, Gyeonggi 410-820, Republic of KoreaGlyceryl monooleate (GMO) is a polar lipid that can exist in various liquid crystalline phases in the presence of different amounts of water. It is regarded as a permeation enhancer due to its amphiphilic property. Various phases of GMO/solvent system containing sodium fluorescein were prepared to compare permeability using confocal laser scanning microscopy (CLSM). GMO was melted in a vial in a water bath heated to 45 °C. Propylene glycol and hexanediol were homogeneously dissolved in the melted GMO. Sodium fluorescein in aqueous solution was diluted to various ratios and thoroughly mixed by an ultrasonic homogenizer. Each GMO/Solvent system with fluorescein was applied onto the epidermal side of excised pig skin and incubated overnight. CLSM was performed to observe how the GMO/solvent system in its different phases affect skin permeability. Cubic and lamellar phase formulations enhanced the fluorescein permeation through the stratum corneum. A solution system had the weakest permeability compared to the other two phases. Due to the amphiphilic nature of GMO, cubic and lamellar phases might reduce the barrier function of stratum corneum which was observed by CLSM as fluorescein accumulated in the dermis. Based on the results, the glyceryl monooleate lyotropic mixtures could be applied to enhance skin permeation in various topical and transdermal formulations.http://www.sciencedirect.com/science/article/pii/S1818087614000683Confocal microscopyImagingGlyceryl monooleateSkin permeationCubic phaseLamellar phase |
| spellingShingle | Dae Gon Lim Won-Wook Jeong Nam Ah Kim Jun Yeul Lim Seol-Hoon Lee Woo Sun Shim Nae-Gyu Kang Seong Hoon Jeong Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging Asian Journal of Pharmaceutical Sciences Confocal microscopy Imaging Glyceryl monooleate Skin permeation Cubic phase Lamellar phase |
| title | Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| title_full | Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| title_fullStr | Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| title_full_unstemmed | Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| title_short | Effect of the glyceryl monooleate-based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| title_sort | effect of the glyceryl monooleate based lyotropic phases on skin permeation using in vitro diffusion and skin imaging |
| topic | Confocal microscopy Imaging Glyceryl monooleate Skin permeation Cubic phase Lamellar phase |
| url | http://www.sciencedirect.com/science/article/pii/S1818087614000683 |
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