Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production
Abstract Colonic mucus layers protect intestinal tissues against intestinal bacteria. We investigated the effects of dietary fiber and its metabolites on mucus production in the colonic mucosa. Mice were fed a partially hydrolyzed guar gum (PHGG)-containing diet and a fiber-free diet (FFD). The colo...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-023-00184-4 |
| _version_ | 1797840807835729920 |
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| author | Mariko Kajiwara-Kubota Kazuhiko Uchiyama Kohei Asaeda Reo Kobayashi Hikaru Hashimoto Takeshi Yasuda Satoshi Sugino Takeshi Sugaya Yasuko Hirai Katsura Mizushima Toshifumi Doi Ken Inoue Osamu Dohi Naohisa Yoshida Takeshi Ishikawa Tomohisa Takagi Hideyuki Konishi Ryo Inoue Yoshito Itoh Yuji Naito |
| author_facet | Mariko Kajiwara-Kubota Kazuhiko Uchiyama Kohei Asaeda Reo Kobayashi Hikaru Hashimoto Takeshi Yasuda Satoshi Sugino Takeshi Sugaya Yasuko Hirai Katsura Mizushima Toshifumi Doi Ken Inoue Osamu Dohi Naohisa Yoshida Takeshi Ishikawa Tomohisa Takagi Hideyuki Konishi Ryo Inoue Yoshito Itoh Yuji Naito |
| author_sort | Mariko Kajiwara-Kubota |
| collection | DOAJ |
| description | Abstract Colonic mucus layers protect intestinal tissues against intestinal bacteria. We investigated the effects of dietary fiber and its metabolites on mucus production in the colonic mucosa. Mice were fed a partially hydrolyzed guar gum (PHGG)-containing diet and a fiber-free diet (FFD). The colon mucus layer, fecal short-chain fatty acid (SCFA) levels, and gut microbiota were evaluated. Mucin 2 (MUC2) expression was assessed in SCFA-treated LS174T cells. The role of AKT in MUC2 production was investigated. The mucus layer in the colonic epithelium was significantly increased in the PHGG group compared with that in the FFD group. In the PHGG group, an increase in Bacteroidetes in the stool was observed, and fecal acetate, butyrate, propionate, and succinate levels were significantly increased. However, MUC2 production was significantly increased only in succinate-stimulated LS174T cells. The succinate-induced MUC2 production was associated with AKT phosphorylation. Succinate mediated the PHGG-induced increase in the colon mucus layer. |
| first_indexed | 2024-04-09T16:20:42Z |
| format | Article |
| id | doaj.art-ace04763e0f64302b9dbde9079fdcfa6 |
| institution | Directory Open Access Journal |
| issn | 2396-8370 |
| language | English |
| last_indexed | 2024-04-09T16:20:42Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj.art-ace04763e0f64302b9dbde9079fdcfa62023-04-23T11:29:26ZengNature Portfolionpj Science of Food2396-83702023-03-01711710.1038/s41538-023-00184-4Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 productionMariko Kajiwara-Kubota0Kazuhiko Uchiyama1Kohei Asaeda2Reo Kobayashi3Hikaru Hashimoto4Takeshi Yasuda5Satoshi Sugino6Takeshi Sugaya7Yasuko Hirai8Katsura Mizushima9Toshifumi Doi10Ken Inoue11Osamu Dohi12Naohisa Yoshida13Takeshi Ishikawa14Tomohisa Takagi15Hideyuki Konishi16Ryo Inoue17Yoshito Itoh18Yuji Naito19Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Medical Regulatory Science, Kyoto Prefectural University of MedicineDepartment of Human Immunology and Nutrition Science, Kyoto Prefectural University of MedicineDepartment of Human Immunology and Nutrition Science, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineLaboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan UniversityDepartment of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of MedicineDepartment of Human Immunology and Nutrition Science, Kyoto Prefectural University of MedicineAbstract Colonic mucus layers protect intestinal tissues against intestinal bacteria. We investigated the effects of dietary fiber and its metabolites on mucus production in the colonic mucosa. Mice were fed a partially hydrolyzed guar gum (PHGG)-containing diet and a fiber-free diet (FFD). The colon mucus layer, fecal short-chain fatty acid (SCFA) levels, and gut microbiota were evaluated. Mucin 2 (MUC2) expression was assessed in SCFA-treated LS174T cells. The role of AKT in MUC2 production was investigated. The mucus layer in the colonic epithelium was significantly increased in the PHGG group compared with that in the FFD group. In the PHGG group, an increase in Bacteroidetes in the stool was observed, and fecal acetate, butyrate, propionate, and succinate levels were significantly increased. However, MUC2 production was significantly increased only in succinate-stimulated LS174T cells. The succinate-induced MUC2 production was associated with AKT phosphorylation. Succinate mediated the PHGG-induced increase in the colon mucus layer.https://doi.org/10.1038/s41538-023-00184-4 |
| spellingShingle | Mariko Kajiwara-Kubota Kazuhiko Uchiyama Kohei Asaeda Reo Kobayashi Hikaru Hashimoto Takeshi Yasuda Satoshi Sugino Takeshi Sugaya Yasuko Hirai Katsura Mizushima Toshifumi Doi Ken Inoue Osamu Dohi Naohisa Yoshida Takeshi Ishikawa Tomohisa Takagi Hideyuki Konishi Ryo Inoue Yoshito Itoh Yuji Naito Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production npj Science of Food |
| title | Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production |
| title_full | Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production |
| title_fullStr | Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production |
| title_full_unstemmed | Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production |
| title_short | Partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate-mediated MUC2 production |
| title_sort | partially hydrolyzed guar gum increased colonic mucus layer in mice via succinate mediated muc2 production |
| url | https://doi.org/10.1038/s41538-023-00184-4 |
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