Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer
Tumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibod...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/11/11/1184 |
| _version_ | 1797509736216657920 |
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| author | Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken |
| author_facet | Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken |
| author_sort | Anne Hoffmann |
| collection | DOAJ |
| description | Tumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibodies directed against myeloperoxidase (MPO) and CD8. Stained specimens were digitalized, and the densities of TANs and CTLs were assessed separately in the mucosa, tumor surface, tumor center, invasion front, and tumor scar. The densities were correlated with clinicopathological patient characteristics. Compared with a historical cohort of 449 treatment naive GCs, the TAN density in the invasion front was significantly lower in neoadjuvantly/perioperatively treated GCs. TAN density in the tumor center and invasion front correlated with tumor regression. TAN density also correlated with CTL density in the tumor center and invasion front. A high density of CTL in the tumor center correlated with an improved overall survival and tumor specific survival. We show that neoadjuvant/perioperative (radio-) chemotherapy impacts on the immune microenvironment of GC, while also depending on sex. The density of TANs in neoadjuvantly/perioperatively treated GCs differed from findings made in a treatment naive GC cohort. |
| first_indexed | 2024-03-10T05:21:59Z |
| format | Article |
| id | doaj.art-ace179e4e8d84356a43868d695c60200 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T05:21:59Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-ace179e4e8d84356a43868d695c602002023-11-22T23:59:11ZengMDPI AGJournal of Personalized Medicine2075-44262021-11-011111118410.3390/jpm11111184Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric CancerAnne Hoffmann0Hans-Michael Behrens1Steffen Heckl2Sandra Krüger3Thomas Becker4Christoph Röcken5Department of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyDepartment of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyDepartment of Internal Medicine II, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyDepartment of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyDepartment of General Surgery, Visceral, Thoracic, Transplantation and Pediatric Surgery, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyDepartment of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, D-24105 Kiel, GermanyTumor associated neutrophils (TANs) and cytotoxic T cells (CTLs) are part of the tumor microenvironment of gastric cancer (GC). We explored their tumor biological significance in neoadjuvantly/perioperatively treated GC. Immunostaining was performed on whole tissue sections of 173 GCs, using antibodies directed against myeloperoxidase (MPO) and CD8. Stained specimens were digitalized, and the densities of TANs and CTLs were assessed separately in the mucosa, tumor surface, tumor center, invasion front, and tumor scar. The densities were correlated with clinicopathological patient characteristics. Compared with a historical cohort of 449 treatment naive GCs, the TAN density in the invasion front was significantly lower in neoadjuvantly/perioperatively treated GCs. TAN density in the tumor center and invasion front correlated with tumor regression. TAN density also correlated with CTL density in the tumor center and invasion front. A high density of CTL in the tumor center correlated with an improved overall survival and tumor specific survival. We show that neoadjuvant/perioperative (radio-) chemotherapy impacts on the immune microenvironment of GC, while also depending on sex. The density of TANs in neoadjuvantly/perioperatively treated GCs differed from findings made in a treatment naive GC cohort.https://www.mdpi.com/2075-4426/11/11/1184gastric cancertumor associated neutrophilscytotoxic T cells |
| spellingShingle | Anne Hoffmann Hans-Michael Behrens Steffen Heckl Sandra Krüger Thomas Becker Christoph Röcken Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer Journal of Personalized Medicine gastric cancer tumor associated neutrophils cytotoxic T cells |
| title | Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer |
| title_full | Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer |
| title_fullStr | Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer |
| title_full_unstemmed | Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer |
| title_short | Neoadjuvant/Perioperative Treatment Affects Spatial Distribution and Densities of Tumor Associated Neutrophils and CD8+ Lymphocytes in Gastric Cancer |
| title_sort | neoadjuvant perioperative treatment affects spatial distribution and densities of tumor associated neutrophils and cd8 lymphocytes in gastric cancer |
| topic | gastric cancer tumor associated neutrophils cytotoxic T cells |
| url | https://www.mdpi.com/2075-4426/11/11/1184 |
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