Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure
The present study was conducted to examine the photosynthetic performance, nutrient status and lipid yield in microalgae Chlorococcum humicola and Chlorella vulgaris under different durations of UV-B exposure (1/2 h, 1 h, 2 h, 3 h and 4 h). Results revealed that UV-B reduces the photosynthetic perfo...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Current Research in Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262819300073 |
| _version_ | 1819146780551413760 |
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| author | Ranjan Singh A.K. Upadhyay Dig Vijay Singh Jay Shankar Singh D.P. Singh |
| author_facet | Ranjan Singh A.K. Upadhyay Dig Vijay Singh Jay Shankar Singh D.P. Singh |
| author_sort | Ranjan Singh |
| collection | DOAJ |
| description | The present study was conducted to examine the photosynthetic performance, nutrient status and lipid yield in microalgae Chlorococcum humicola and Chlorella vulgaris under different durations of UV-B exposure (1/2 h, 1 h, 2 h, 3 h and 4 h). Results revealed that UV-B reduces the photosynthetic performance of photosystem II by altering photosynthetic performance index (Pi ABS), maximum quantum yield (Fv/Fm), net closing rate of reaction centre (Mo), trapping flux (TRo/RC) and effective antenna size with their respective control. Scanning Electron Microscopy (SEM) exhibited variegated structure and increased cell size by ~15–65% which was more pronounced in the case of C. humicola at 4 h of UV-B. The Energy Dispersive Spectroscopy (EDS) data showed that the content of microelements (C, O, Na, and K) in terms of atomic weight % was found to be significantly increased in C. vulgaris while with C. humicola, it was restricted to carbon (C) only. Further, the high antioxidant (ascorbic acid, cysteine and proline) potential and carotenoid/chl a photoprotection response reflects protection against UV-B in the both algae. In the case of lipid, comparative greater increase in cell size of C. humicola correlated with high lipid yield as compare to C. vulgaris at 2 h of UV-B could be employed in the production of biofuel in sustainable manner. Thus, algae C. humicola could be a best alternative feed stock of lipid and biofuel production in the area receiving high solar radiation. |
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| format | Article |
| id | doaj.art-ace213efe48d4b688f229405bf8f1ac6 |
| institution | Directory Open Access Journal |
| issn | 2590-2628 |
| language | English |
| last_indexed | 2024-12-22T13:19:22Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Biotechnology |
| spelling | doaj.art-ace213efe48d4b688f229405bf8f1ac62022-12-21T18:24:30ZengElsevierCurrent Research in Biotechnology2590-26282019-11-0116577Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposureRanjan Singh0A.K. Upadhyay1Dig Vijay Singh2Jay Shankar Singh3D.P. Singh4Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, IndiaDepartment of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, IndiaDepartment of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, IndiaDepartment of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, IndiaDepartment of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow 226025, India; Corresponding author.The present study was conducted to examine the photosynthetic performance, nutrient status and lipid yield in microalgae Chlorococcum humicola and Chlorella vulgaris under different durations of UV-B exposure (1/2 h, 1 h, 2 h, 3 h and 4 h). Results revealed that UV-B reduces the photosynthetic performance of photosystem II by altering photosynthetic performance index (Pi ABS), maximum quantum yield (Fv/Fm), net closing rate of reaction centre (Mo), trapping flux (TRo/RC) and effective antenna size with their respective control. Scanning Electron Microscopy (SEM) exhibited variegated structure and increased cell size by ~15–65% which was more pronounced in the case of C. humicola at 4 h of UV-B. The Energy Dispersive Spectroscopy (EDS) data showed that the content of microelements (C, O, Na, and K) in terms of atomic weight % was found to be significantly increased in C. vulgaris while with C. humicola, it was restricted to carbon (C) only. Further, the high antioxidant (ascorbic acid, cysteine and proline) potential and carotenoid/chl a photoprotection response reflects protection against UV-B in the both algae. In the case of lipid, comparative greater increase in cell size of C. humicola correlated with high lipid yield as compare to C. vulgaris at 2 h of UV-B could be employed in the production of biofuel in sustainable manner. Thus, algae C. humicola could be a best alternative feed stock of lipid and biofuel production in the area receiving high solar radiation.http://www.sciencedirect.com/science/article/pii/S2590262819300073UV-BChlorophyll fluorescenceNutrient statusLipid yieldSEM-EDS |
| spellingShingle | Ranjan Singh A.K. Upadhyay Dig Vijay Singh Jay Shankar Singh D.P. Singh Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure Current Research in Biotechnology UV-B Chlorophyll fluorescence Nutrient status Lipid yield SEM-EDS |
| title | Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure |
| title_full | Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure |
| title_fullStr | Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure |
| title_full_unstemmed | Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure |
| title_short | Photosynthetic performance, nutrient status and lipid yield of microalgae Chlorella vulgaris and Chlorococcum humicola under UV-B exposure |
| title_sort | photosynthetic performance nutrient status and lipid yield of microalgae chlorella vulgaris and chlorococcum humicola under uv b exposure |
| topic | UV-B Chlorophyll fluorescence Nutrient status Lipid yield SEM-EDS |
| url | http://www.sciencedirect.com/science/article/pii/S2590262819300073 |
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