Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population
(1) Background: Lysyl oxidase (LOX) plays a dual role in carcinogenesis and studies show a higher risk of cancer in LOX G473A variants. The present study evaluated the pattern of LOX G473A polymorphism (rs1800449) and serum LOX levels in ovarian cancer patients. (2) Methods: Serum LOX levels were es...
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MDPI AG
2021-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/1/53 |
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| author | Suchitra Kumari A. Raj Kumar Patro Baijayantimala Mishra Saubhagya Kumar Jena Sweta Singh |
| author_facet | Suchitra Kumari A. Raj Kumar Patro Baijayantimala Mishra Saubhagya Kumar Jena Sweta Singh |
| author_sort | Suchitra Kumari |
| collection | DOAJ |
| description | (1) Background: Lysyl oxidase (LOX) plays a dual role in carcinogenesis and studies show a higher risk of cancer in LOX G473A variants. The present study evaluated the pattern of LOX G473A polymorphism (rs1800449) and serum LOX levels in ovarian cancer patients. (2) Methods: Serum LOX levels were estimated by enzyme linked immunosorbent assay (ELISA). A polymorphism of rs1800449 of LOX gene was detected by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Selected samples were sequenced for external validation. (3) Results: A majority of study participants were from low socio-economic status. Serum LOX level was significantly higher in ovarian cancer patients as compared to control. Serum LOX level in early-stage ovarian cancer was significantly lower as compared to advanced stage (FIGO stage III & IV). Wild type GG genotype was used as reference. Genotypes AA were associated with a significant risk of epithelial ovarian cancer (OR 3.208; <i>p</i> value- 0.033). A allele of rs1800449 polymorphism of LOX gene, the odds ratio was 1.866 (95% Confidence Interval 1.112–3.16) <i>p</i> value = 0.017 (4) Conclusions: A allele of rs1800449 polymorphism of LOX gene presents an increased risk of ovarian cancer in East Indian population. Serum LOX levels could be a potential biomarker for the diagnosis and prognosis of ovarian cancer. |
| first_indexed | 2024-03-10T01:39:37Z |
| format | Article |
| id | doaj.art-ace28aa44fd34ca895e223e3e5a64c41 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T01:39:37Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-ace28aa44fd34ca895e223e3e5a64c412023-11-23T13:27:25ZengMDPI AGDiagnostics2075-44182021-12-011215310.3390/diagnostics12010053Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian PopulationSuchitra Kumari0A. Raj Kumar Patro1Baijayantimala Mishra2Saubhagya Kumar Jena3Sweta Singh4Department of Biochemistry, All India Institute of Medical Sciences, Bhubaneswar 751019, IndiaMolecular Biology Laboratory, Department of Microbiology, Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar 751024, IndiaDepartment of Microbiology, All India Institute of Medical Sciences, Bhubaneswar 751019, IndiaDepartment of Obstetrics & Gynaecology, All India Institute of Medical Sciences, Bhubaneswar 751019, IndiaDepartment of Obstetrics & Gynaecology, All India Institute of Medical Sciences, Bhubaneswar 751019, India(1) Background: Lysyl oxidase (LOX) plays a dual role in carcinogenesis and studies show a higher risk of cancer in LOX G473A variants. The present study evaluated the pattern of LOX G473A polymorphism (rs1800449) and serum LOX levels in ovarian cancer patients. (2) Methods: Serum LOX levels were estimated by enzyme linked immunosorbent assay (ELISA). A polymorphism of rs1800449 of LOX gene was detected by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Selected samples were sequenced for external validation. (3) Results: A majority of study participants were from low socio-economic status. Serum LOX level was significantly higher in ovarian cancer patients as compared to control. Serum LOX level in early-stage ovarian cancer was significantly lower as compared to advanced stage (FIGO stage III & IV). Wild type GG genotype was used as reference. Genotypes AA were associated with a significant risk of epithelial ovarian cancer (OR 3.208; <i>p</i> value- 0.033). A allele of rs1800449 polymorphism of LOX gene, the odds ratio was 1.866 (95% Confidence Interval 1.112–3.16) <i>p</i> value = 0.017 (4) Conclusions: A allele of rs1800449 polymorphism of LOX gene presents an increased risk of ovarian cancer in East Indian population. Serum LOX levels could be a potential biomarker for the diagnosis and prognosis of ovarian cancer.https://www.mdpi.com/2075-4418/12/1/53lysyl oxidasesingle nucleotide polymorphismrestriction fragment length polymorphismovarian cancer |
| spellingShingle | Suchitra Kumari A. Raj Kumar Patro Baijayantimala Mishra Saubhagya Kumar Jena Sweta Singh Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population Diagnostics lysyl oxidase single nucleotide polymorphism restriction fragment length polymorphism ovarian cancer |
| title | Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population |
| title_full | Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population |
| title_fullStr | Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population |
| title_full_unstemmed | Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population |
| title_short | Serum Lysyl Oxidase Levels and Lysyl Oxidase Gene Polymorphism in Ovarian Cancer Patients of Eastern Indian Population |
| title_sort | serum lysyl oxidase levels and lysyl oxidase gene polymorphism in ovarian cancer patients of eastern indian population |
| topic | lysyl oxidase single nucleotide polymorphism restriction fragment length polymorphism ovarian cancer |
| url | https://www.mdpi.com/2075-4418/12/1/53 |
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