Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis
Ultrasound imaging is a highly valuable tool in imaging human tissues due to its non-invasive and easily accessible nature. Despite advances in the field of ultrasound research, conventional transducers with frequencies lower than 20 MHz face limitations in resolution for cellular applications. To a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/4/1916 |
| _version_ | 1797618296763187200 |
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| author | Hayong Jung K. Kirk Shung Hae Gyun Lim |
| author_facet | Hayong Jung K. Kirk Shung Hae Gyun Lim |
| author_sort | Hayong Jung |
| collection | DOAJ |
| description | Ultrasound imaging is a highly valuable tool in imaging human tissues due to its non-invasive and easily accessible nature. Despite advances in the field of ultrasound research, conventional transducers with frequencies lower than 20 MHz face limitations in resolution for cellular applications. To address this challenge, we employed ultrahigh frequency (UHF) transducers and demonstrated their potential applications in the field of biomedical engineering, specifically for cell imaging and acoustic tweezers. The lateral resolution achieved with a 110 MHz UHF transducer was 20 μm, and 6.5 μm with a 410 MHz transducer, which is capable of imaging single cells. The results of our experiments demonstrated the successful imaging of a single PC-3 cell and a 15 μm bead using an acoustic scanning microscope equipped with UHF transducers. Additionally, the dual-mode multifunctional UHF transducer was used to trap and manipulate single cells and beads, highlighting its potential for single-cell studies in areas such as cell deformability and mechanotransduction. |
| first_indexed | 2024-03-11T08:11:06Z |
| format | Article |
| id | doaj.art-ace541ddaaf4443985ee8d867ee8cd78 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T08:11:06Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ace541ddaaf4443985ee8d867ee8cd782023-11-16T23:07:51ZengMDPI AGSensors1424-82202023-02-01234191610.3390/s23041916Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell AnalysisHayong Jung0K. Kirk Shung1Hae Gyun Lim2Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USADepartment of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of KoreaUltrasound imaging is a highly valuable tool in imaging human tissues due to its non-invasive and easily accessible nature. Despite advances in the field of ultrasound research, conventional transducers with frequencies lower than 20 MHz face limitations in resolution for cellular applications. To address this challenge, we employed ultrahigh frequency (UHF) transducers and demonstrated their potential applications in the field of biomedical engineering, specifically for cell imaging and acoustic tweezers. The lateral resolution achieved with a 110 MHz UHF transducer was 20 μm, and 6.5 μm with a 410 MHz transducer, which is capable of imaging single cells. The results of our experiments demonstrated the successful imaging of a single PC-3 cell and a 15 μm bead using an acoustic scanning microscope equipped with UHF transducers. Additionally, the dual-mode multifunctional UHF transducer was used to trap and manipulate single cells and beads, highlighting its potential for single-cell studies in areas such as cell deformability and mechanotransduction.https://www.mdpi.com/1424-8220/23/4/1916ultrasoundB-mode imagingsingle-cell imagingacoustic tweezerultrahigh frequency transducer |
| spellingShingle | Hayong Jung K. Kirk Shung Hae Gyun Lim Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis Sensors ultrasound B-mode imaging single-cell imaging acoustic tweezer ultrahigh frequency transducer |
| title | Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis |
| title_full | Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis |
| title_fullStr | Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis |
| title_full_unstemmed | Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis |
| title_short | Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis |
| title_sort | ultrasonic high resolution imaging and acoustic tweezers using ultrahigh frequency transducer integrative single cell analysis |
| topic | ultrasound B-mode imaging single-cell imaging acoustic tweezer ultrahigh frequency transducer |
| url | https://www.mdpi.com/1424-8220/23/4/1916 |
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