<i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells
The association between lactic acid bacteria (LAB) and their immunostimulatory effects has attracted considerable attention; however, it remains unclear whether LAB can induce interferon-lambdas (IFN-λs) in human epithelial cells under conditions that do not mimic infection. In this study, we first...
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/827 |
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| author | Shohei Satomi Daichi Kokubu Takuro Inoue Masaya Sugiyama Masashi Mizokami Shigenori Suzuki Kazumoto Murata |
| author_facet | Shohei Satomi Daichi Kokubu Takuro Inoue Masaya Sugiyama Masashi Mizokami Shigenori Suzuki Kazumoto Murata |
| author_sort | Shohei Satomi |
| collection | DOAJ |
| description | The association between lactic acid bacteria (LAB) and their immunostimulatory effects has attracted considerable attention; however, it remains unclear whether LAB can induce interferon-lambdas (IFN-λs) in human epithelial cells under conditions that do not mimic infection. In this study, we first employed a reporter assay to screen for a potential strain capable of inducing IFN-λ3 among 135 LAB strains derived from traditional Japanese pickles. Next, we assessed the strain’s ability to induce the expression of IFN-λ genes and interferon-stimulated genes (ISGs), and to produce IFN-λs. As a result, we screened and isolated <i>Enterococcus casseliflavus</i> KB1733 (KB1733) as a potential strain capable of inducing IFN-λ3 expression. Furthermore, we clarified that KB1733 induced the expression of IFN-λ genes and ISGs related to antiviral functions, and that KB1733 induced IFN-λ1 and -λ3 expression in a dose-dependent manner up to 10 μg/mL. In addition, KB1733 significantly increased IFN-λ1 production compared to <i>Enterococcus casseliflavus</i> JCM8723<sup>T</sup>, which belongs to the same genera and species as KB1733. In conclusion, we isolated a unique LAB strain from traditional Japanese pickles that is capable of stimulating IFN-λ production, although further study is needed to investigate how KB1733 protects against viruses in mice and humans. |
| first_indexed | 2024-03-09T04:23:10Z |
| format | Article |
| id | doaj.art-acec41fe11164ecd9b2230abfe320f4b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:23:10Z |
| publishDate | 2022-04-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-acec41fe11164ecd9b2230abfe320f4b2023-12-03T13:45:19ZengMDPI AGMicroorganisms2076-26072022-04-0110482710.3390/microorganisms10040827<i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial CellsShohei Satomi0Daichi Kokubu1Takuro Inoue2Masaya Sugiyama3Masashi Mizokami4Shigenori Suzuki5Kazumoto Murata6Department of Nature & Wellness Research, Innovation Division, KAGOME CO., LTD., 17 Nishitomiyama, Nasushiobara 329-2762, Tochigi, JapanDepartment of Nature & Wellness Research, Innovation Division, KAGOME CO., LTD., 17 Nishitomiyama, Nasushiobara 329-2762, Tochigi, JapanDepartment of Nature & Wellness Research, Innovation Division, KAGOME CO., LTD., 17 Nishitomiyama, Nasushiobara 329-2762, Tochigi, JapanGenome Medical Science Project, National Center for Global Health and Medicine, 1-7-1 Kohnodai, Ichikawa 272-8516, Chiba, JapanGenome Medical Science Project, National Center for Global Health and Medicine, 1-7-1 Kohnodai, Ichikawa 272-8516, Chiba, JapanDepartment of Nature & Wellness Research, Innovation Division, KAGOME CO., LTD., 17 Nishitomiyama, Nasushiobara 329-2762, Tochigi, JapanGenome Medical Science Project, National Center for Global Health and Medicine, 1-7-1 Kohnodai, Ichikawa 272-8516, Chiba, JapanThe association between lactic acid bacteria (LAB) and their immunostimulatory effects has attracted considerable attention; however, it remains unclear whether LAB can induce interferon-lambdas (IFN-λs) in human epithelial cells under conditions that do not mimic infection. In this study, we first employed a reporter assay to screen for a potential strain capable of inducing IFN-λ3 among 135 LAB strains derived from traditional Japanese pickles. Next, we assessed the strain’s ability to induce the expression of IFN-λ genes and interferon-stimulated genes (ISGs), and to produce IFN-λs. As a result, we screened and isolated <i>Enterococcus casseliflavus</i> KB1733 (KB1733) as a potential strain capable of inducing IFN-λ3 expression. Furthermore, we clarified that KB1733 induced the expression of IFN-λ genes and ISGs related to antiviral functions, and that KB1733 induced IFN-λ1 and -λ3 expression in a dose-dependent manner up to 10 μg/mL. In addition, KB1733 significantly increased IFN-λ1 production compared to <i>Enterococcus casseliflavus</i> JCM8723<sup>T</sup>, which belongs to the same genera and species as KB1733. In conclusion, we isolated a unique LAB strain from traditional Japanese pickles that is capable of stimulating IFN-λ production, although further study is needed to investigate how KB1733 protects against viruses in mice and humans.https://www.mdpi.com/2076-2607/10/4/827screeningpickles<i>Enterococcus casseliflavus</i> KB1733heat-killed bacteriainterferon-lambda |
| spellingShingle | Shohei Satomi Daichi Kokubu Takuro Inoue Masaya Sugiyama Masashi Mizokami Shigenori Suzuki Kazumoto Murata <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells Microorganisms screening pickles <i>Enterococcus casseliflavus</i> KB1733 heat-killed bacteria interferon-lambda |
| title | <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells |
| title_full | <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells |
| title_fullStr | <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells |
| title_full_unstemmed | <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells |
| title_short | <i>Enterococcus casseliflavus</i> KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells |
| title_sort | i enterococcus casseliflavus i kb1733 isolated from a traditional japanese pickle induces interferon lambda production in human intestinal epithelial cells |
| topic | screening pickles <i>Enterococcus casseliflavus</i> KB1733 heat-killed bacteria interferon-lambda |
| url | https://www.mdpi.com/2076-2607/10/4/827 |
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