A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels
Ovothiols are histidine-derived thiols produced by a variety of marine invertebrates, protists and bacteria. These compounds, which are among the strongest natural antioxidants, are involved in controlling the cellular redox balance due to their redox exchange with glutathione. Although ovothiols we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/4/268 |
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| author | Carola Murano Annalisa Zuccarotto Serena Leone Marco Sollitto Marco Gerdol Immacolata Castellano Anna Palumbo |
| author_facet | Carola Murano Annalisa Zuccarotto Serena Leone Marco Sollitto Marco Gerdol Immacolata Castellano Anna Palumbo |
| author_sort | Carola Murano |
| collection | DOAJ |
| description | Ovothiols are histidine-derived thiols produced by a variety of marine invertebrates, protists and bacteria. These compounds, which are among the strongest natural antioxidants, are involved in controlling the cellular redox balance due to their redox exchange with glutathione. Although ovothiols were initially reported as protective agents against environmental stressors, new evidence suggests that they can also act as pheromones and participate in fundamental biological processes such as embryogenesis. To get further insight into the biological roles of ovothiols, we compared ovothiol biosynthesis in the sea urchin <i>Paracentrotus lividus</i> and in the mussel <i>Mytilus galloprovincialis</i>, the two species that represent the richest sources of these compounds among marine invertebrates. Ovothiol content was measured in different tissues and in the immune cells from both species and the expression levels of <i>ovoA</i>, the gene responsible for ovothiol biosynthesis, was inferred from publicly available transcriptomes. A comparative analysis of ovothiol biosynthesis in the two species allowed the identification of the tissues and cells synthesizing the metabolite and highlighted analogies and differences between sea urchins and mussels. By improving our knowledge on the biological roles of ovothiols and pointing out the existence of sustainable natural sources for their isolation, this study provides the basis for future biotechnological investigations on these valuable compounds. |
| first_indexed | 2024-03-09T13:23:36Z |
| format | Article |
| id | doaj.art-acf1434539634aedb3340f2c0bd5f61a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T13:23:36Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-acf1434539634aedb3340f2c0bd5f61a2023-11-30T21:27:06ZengMDPI AGMarine Drugs1660-33972022-04-0120426810.3390/md20040268A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and MusselsCarola Murano0Annalisa Zuccarotto1Serena Leone2Marco Sollitto3Marco Gerdol4Immacolata Castellano5Anna Palumbo6Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, 80121 Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, 80121 Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, 80121 Naples, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, 80121 Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, 80121 Naples, ItalyOvothiols are histidine-derived thiols produced by a variety of marine invertebrates, protists and bacteria. These compounds, which are among the strongest natural antioxidants, are involved in controlling the cellular redox balance due to their redox exchange with glutathione. Although ovothiols were initially reported as protective agents against environmental stressors, new evidence suggests that they can also act as pheromones and participate in fundamental biological processes such as embryogenesis. To get further insight into the biological roles of ovothiols, we compared ovothiol biosynthesis in the sea urchin <i>Paracentrotus lividus</i> and in the mussel <i>Mytilus galloprovincialis</i>, the two species that represent the richest sources of these compounds among marine invertebrates. Ovothiol content was measured in different tissues and in the immune cells from both species and the expression levels of <i>ovoA</i>, the gene responsible for ovothiol biosynthesis, was inferred from publicly available transcriptomes. A comparative analysis of ovothiol biosynthesis in the two species allowed the identification of the tissues and cells synthesizing the metabolite and highlighted analogies and differences between sea urchins and mussels. By improving our knowledge on the biological roles of ovothiols and pointing out the existence of sustainable natural sources for their isolation, this study provides the basis for future biotechnological investigations on these valuable compounds.https://www.mdpi.com/1660-3397/20/4/268ovothiolsea urchinmusselantioxidantoxidative stress |
| spellingShingle | Carola Murano Annalisa Zuccarotto Serena Leone Marco Sollitto Marco Gerdol Immacolata Castellano Anna Palumbo A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels Marine Drugs ovothiol sea urchin mussel antioxidant oxidative stress |
| title | A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels |
| title_full | A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels |
| title_fullStr | A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels |
| title_full_unstemmed | A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels |
| title_short | A Survey on the Distribution of Ovothiol and <i>ovoA</i> Gene Expression in Different Tissues and Cells: A Comparative Analysis in Sea Urchins and Mussels |
| title_sort | survey on the distribution of ovothiol and i ovoa i gene expression in different tissues and cells a comparative analysis in sea urchins and mussels |
| topic | ovothiol sea urchin mussel antioxidant oxidative stress |
| url | https://www.mdpi.com/1660-3397/20/4/268 |
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