The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety
The present work shows the biochemical and structural fundamentals for the stress induced anxiety and stress adjustment response of the CA3 hippocampus area. Adult male Wistar rats were repeatedly exposed to a 3 h day restraint stress, for either 3 or 6 days. The concentration of corticosterone and...
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| Format: | Article |
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MDPI AG
2022-08-01
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| author | Vlad-Alexandru Toma Bogdan Dume Rareș Trâncă Bogdan Sevastre Lucian Barbu Gabriela Adriana Filip Ioana Roman Alexandra-Cristina Sevastre-Berghian |
| author_facet | Vlad-Alexandru Toma Bogdan Dume Rareș Trâncă Bogdan Sevastre Lucian Barbu Gabriela Adriana Filip Ioana Roman Alexandra-Cristina Sevastre-Berghian |
| author_sort | Vlad-Alexandru Toma |
| collection | DOAJ |
| description | The present work shows the biochemical and structural fundamentals for the stress induced anxiety and stress adjustment response of the CA3 hippocampus area. Adult male Wistar rats were repeatedly exposed to a 3 h day restraint stress, for either 3 or 6 days. The concentration of corticosterone and testosterone in the CA3 hippocampus area was divergent, while oxidative stress was progressively increased during the stress exposure. The mitochondrial lysis in the CA3 neurons confirmed the oxidative stress events. Immunohistochemical findings showed that oligodendrocytes (OCs) proliferation and neuroglobin (Ngb) expression were stimulated, whereas MeCP2 expression was decreased as a balance reaction in stress exposure under corticosterone signaling. Remarkably, ultrastructural changes such as mitochondrial lysis, endoplasmic reticulum swelling, and perivascular lysis with platelets adherence to endothelium in the CA3 area were seen in the 6th day of restraining. The anxiety-like behavior was noticed 6 days later after stress exposure. These results suggest that the duration of the exposure, but not the intensity of the stress, is the key factor in the stress-buffering function by the CA3 hippocampus area via up-regulation of the Ngb-OCs bionome. The imbalance of the Ngb-OCs communication may be involved in the development of CA3-dependent anxious behavior. |
| first_indexed | 2024-03-10T03:02:02Z |
| format | Article |
| id | doaj.art-acf1913696c14dcaac094b8be043fea8 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:02:02Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-acf1913696c14dcaac094b8be043fea82023-11-23T12:44:50ZengMDPI AGApplied Sciences2076-34172022-08-011217868010.3390/app12178680The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced AnxietyVlad-Alexandru Toma0Bogdan Dume1Rareș Trâncă2Bogdan Sevastre3Lucian Barbu4Gabriela Adriana Filip5Ioana Roman6Alexandra-Cristina Sevastre-Berghian7Department of Molecular Biology and Biotechnology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaParaclinic/Clinic Department, Faculty of Veterinary Madicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Babeș-Bolyai University, 400347 Cluj-Napoca, RomaniaFaculty of Medicine, University of Medicine and Pharmacy ”Iuliu Hațieganu”, 400347 Cluj-Napoca, RomaniaInstitute of Biological Research, Branch of NIRDBS Bucharest, 400015 Cluj-Napoca, RomaniaFaculty of Medicine, University of Medicine and Pharmacy ”Iuliu Hațieganu”, 400347 Cluj-Napoca, RomaniaThe present work shows the biochemical and structural fundamentals for the stress induced anxiety and stress adjustment response of the CA3 hippocampus area. Adult male Wistar rats were repeatedly exposed to a 3 h day restraint stress, for either 3 or 6 days. The concentration of corticosterone and testosterone in the CA3 hippocampus area was divergent, while oxidative stress was progressively increased during the stress exposure. The mitochondrial lysis in the CA3 neurons confirmed the oxidative stress events. Immunohistochemical findings showed that oligodendrocytes (OCs) proliferation and neuroglobin (Ngb) expression were stimulated, whereas MeCP2 expression was decreased as a balance reaction in stress exposure under corticosterone signaling. Remarkably, ultrastructural changes such as mitochondrial lysis, endoplasmic reticulum swelling, and perivascular lysis with platelets adherence to endothelium in the CA3 area were seen in the 6th day of restraining. The anxiety-like behavior was noticed 6 days later after stress exposure. These results suggest that the duration of the exposure, but not the intensity of the stress, is the key factor in the stress-buffering function by the CA3 hippocampus area via up-regulation of the Ngb-OCs bionome. The imbalance of the Ngb-OCs communication may be involved in the development of CA3-dependent anxious behavior.https://www.mdpi.com/2076-3417/12/17/8680neuroglobinCA3 pyramidal neuronsstressbuffering reactionsanxiety |
| spellingShingle | Vlad-Alexandru Toma Bogdan Dume Rareș Trâncă Bogdan Sevastre Lucian Barbu Gabriela Adriana Filip Ioana Roman Alexandra-Cristina Sevastre-Berghian The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety Applied Sciences neuroglobin CA3 pyramidal neurons stress buffering reactions anxiety |
| title | The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety |
| title_full | The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety |
| title_fullStr | The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety |
| title_full_unstemmed | The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety |
| title_short | The Effect of Repeated Restraint Stress on Neuroglobin-Oligodendrocytes Functions in the CA3 Hippocampal Area and Their Involvements in the Signaling Pathways of the Stress-Induced Anxiety |
| title_sort | effect of repeated restraint stress on neuroglobin oligodendrocytes functions in the ca3 hippocampal area and their involvements in the signaling pathways of the stress induced anxiety |
| topic | neuroglobin CA3 pyramidal neurons stress buffering reactions anxiety |
| url | https://www.mdpi.com/2076-3417/12/17/8680 |
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