ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity
Since the start of the COVID-19 pandemic, many studies have investigated the humoral response to SARS-CoV-2 during infection. Studies with native viral proteins constitute a first-line approach to assessing the overall immune response, but small peptides are an accurate and valuable tool for the fin...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/11/2/405 |
| _version_ | 1797492979079839744 |
|---|---|
| author | Charline Herrscher Sébastien Eymieux Christophe Gaborit Hélène Blasco Julien Marlet Karl Stefic Philippe Roingeard Leslie Grammatico-Guillon Christophe Hourioux |
| author_facet | Charline Herrscher Sébastien Eymieux Christophe Gaborit Hélène Blasco Julien Marlet Karl Stefic Philippe Roingeard Leslie Grammatico-Guillon Christophe Hourioux |
| author_sort | Charline Herrscher |
| collection | DOAJ |
| description | Since the start of the COVID-19 pandemic, many studies have investigated the humoral response to SARS-CoV-2 during infection. Studies with native viral proteins constitute a first-line approach to assessing the overall immune response, but small peptides are an accurate and valuable tool for the fine characterization of B-cell epitopes, despite the restriction of this approach to the determination of linear epitopes. In this study, we used ELISA and peptides covering a selection of structural and non-structural SARS-CoV-2 proteins to identify key epitopes eliciting a strong immune response that could serve as a biological signature of disease characteristics, such as severity, in particular. We used 213 plasma samples from a cohort of patients well-characterized clinically and biologically and followed for COVID-19 infection. We found that patients developing severe disease had higher titers of antibodies mapping to multiple specific epitopes than patients with mild to moderate disease. These data are potentially important as they could be used for immunological profiling to improve our knowledge of the quantitative and qualitative characteristics of the humoral response in relation to patient outcome. |
| first_indexed | 2024-03-10T01:13:24Z |
| format | Article |
| id | doaj.art-acf3efb337cb46a2825e6187e3b3887f |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T01:13:24Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-acf3efb337cb46a2825e6187e3b3887f2023-11-23T14:13:27ZengMDPI AGJournal of Clinical Medicine2077-03832022-01-0111240510.3390/jcm11020405ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease SeverityCharline Herrscher0Sébastien Eymieux1Christophe Gaborit2Hélène Blasco3Julien Marlet4Karl Stefic5Philippe Roingeard6Leslie Grammatico-Guillon7Christophe Hourioux8Inserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceEpidémiologie des Données Cliniques en Centre-Val de Loire (EpiDcliC), Centre Hospitalier Universitaire de Tours, 37000 Tours, FranceService de Biochimie et Biologie Moléculaire, CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceInserm U1259 Morphogénèse et Antigénicité du VIH et des virus des Hépatites, Université de Tours et CHRU de Tours, 37000 Tours, FranceSince the start of the COVID-19 pandemic, many studies have investigated the humoral response to SARS-CoV-2 during infection. Studies with native viral proteins constitute a first-line approach to assessing the overall immune response, but small peptides are an accurate and valuable tool for the fine characterization of B-cell epitopes, despite the restriction of this approach to the determination of linear epitopes. In this study, we used ELISA and peptides covering a selection of structural and non-structural SARS-CoV-2 proteins to identify key epitopes eliciting a strong immune response that could serve as a biological signature of disease characteristics, such as severity, in particular. We used 213 plasma samples from a cohort of patients well-characterized clinically and biologically and followed for COVID-19 infection. We found that patients developing severe disease had higher titers of antibodies mapping to multiple specific epitopes than patients with mild to moderate disease. These data are potentially important as they could be used for immunological profiling to improve our knowledge of the quantitative and qualitative characteristics of the humoral response in relation to patient outcome.https://www.mdpi.com/2077-0383/11/2/405SARS-CoV-2 antibodiesSARS-CoV-2 linear epitopesCOVID-19disease severity |
| spellingShingle | Charline Herrscher Sébastien Eymieux Christophe Gaborit Hélène Blasco Julien Marlet Karl Stefic Philippe Roingeard Leslie Grammatico-Guillon Christophe Hourioux ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity Journal of Clinical Medicine SARS-CoV-2 antibodies SARS-CoV-2 linear epitopes COVID-19 disease severity |
| title | ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity |
| title_full | ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity |
| title_fullStr | ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity |
| title_full_unstemmed | ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity |
| title_short | ELISA-Based Analysis Reveals an Anti-SARS-CoV-2 Protein Immune Response Profile Associated with Disease Severity |
| title_sort | elisa based analysis reveals an anti sars cov 2 protein immune response profile associated with disease severity |
| topic | SARS-CoV-2 antibodies SARS-CoV-2 linear epitopes COVID-19 disease severity |
| url | https://www.mdpi.com/2077-0383/11/2/405 |
| work_keys_str_mv | AT charlineherrscher elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT sebastieneymieux elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT christophegaborit elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT heleneblasco elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT julienmarlet elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT karlstefic elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT philipperoingeard elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT lesliegrammaticoguillon elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity AT christophehourioux elisabasedanalysisrevealsanantisarscov2proteinimmuneresponseprofileassociatedwithdiseaseseverity |