Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases
Mass transfer in nanocomposite hydrogel thin films consisting of alternating layers of an organometallic redox polymer (RP) and oxidoreductase enzymes was investigated. Multilayer nanostructures were fabricated on gold surfaces by the deposition of an anionic self-assembled monolayer of 11-mercaptou...
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| Format: | Article |
| Language: | English |
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MDPI AG
2002-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/2/3/79/ |
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| author | Aleksandr L. Simonian Alexander Revzin Michael V. Pishko |
| author_facet | Aleksandr L. Simonian Alexander Revzin Michael V. Pishko |
| author_sort | Aleksandr L. Simonian |
| collection | DOAJ |
| description | Mass transfer in nanocomposite hydrogel thin films consisting of alternating layers of an organometallic redox polymer (RP) and oxidoreductase enzymes was investigated. Multilayer nanostructures were fabricated on gold surfaces by the deposition of an anionic self-assembled monolayer of 11-mercaptoundecanoic acid, followed by the electrostatic binding of a cationic redox polymer, poly[vinylpyridine Os(bis-bipyridine)2Clco-allylamine], and an anionic oxidoreductase. Surface plasmon resonance spectroscopy, Fourier transform infrared external reflection spectroscopy (FTIR-ERS), ellipsometry and electrochemistry were employed to characterize the assembly of these nanocomposite films. Simultaneous SPR/electrochemistry enabled real time observation of the assembly of sensing components, changes in film structure with electrode potential, and the immediate, in situ electrochemical verification of substrate-dependent current upon the addition of enzyme to the multilayer structure. SPR and FTIR-ERS studies also showed no desorption of polymer or enzyme from the nanocomposite structure when stored in aqueous environment occurred over the period of three weeks, suggesting that decreasing in substrate sensitivity were due to loss of enzymatic activity rather than loss of film compounds from the nanostructure. |
| first_indexed | 2024-04-14T03:34:03Z |
| format | Article |
| id | doaj.art-acf48c3a9c5e43358f82d8a2b0982578 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T03:34:03Z |
| publishDate | 2002-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-acf48c3a9c5e43358f82d8a2b09825782022-12-22T02:14:51ZengMDPI AGSensors1424-82202002-03-0123799010.3390/s20300079Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and OxidoreductasesAleksandr L. SimonianAlexander RevzinMichael V. PishkoMass transfer in nanocomposite hydrogel thin films consisting of alternating layers of an organometallic redox polymer (RP) and oxidoreductase enzymes was investigated. Multilayer nanostructures were fabricated on gold surfaces by the deposition of an anionic self-assembled monolayer of 11-mercaptoundecanoic acid, followed by the electrostatic binding of a cationic redox polymer, poly[vinylpyridine Os(bis-bipyridine)2Clco-allylamine], and an anionic oxidoreductase. Surface plasmon resonance spectroscopy, Fourier transform infrared external reflection spectroscopy (FTIR-ERS), ellipsometry and electrochemistry were employed to characterize the assembly of these nanocomposite films. Simultaneous SPR/electrochemistry enabled real time observation of the assembly of sensing components, changes in film structure with electrode potential, and the immediate, in situ electrochemical verification of substrate-dependent current upon the addition of enzyme to the multilayer structure. SPR and FTIR-ERS studies also showed no desorption of polymer or enzyme from the nanocomposite structure when stored in aqueous environment occurred over the period of three weeks, suggesting that decreasing in substrate sensitivity were due to loss of enzymatic activity rather than loss of film compounds from the nanostructure.http://www.mdpi.com/1424-8220/2/3/79/BiosensorRedoxNanocompositeHydrogelEnzyme |
| spellingShingle | Aleksandr L. Simonian Alexander Revzin Michael V. Pishko Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases Sensors Biosensor Redox Nanocomposite Hydrogel Enzyme |
| title | Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases |
| title_full | Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases |
| title_fullStr | Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases |
| title_full_unstemmed | Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases |
| title_short | Mass Transfer in Amperometric Biosensors Based on Nanocomposite Thin Films of Redox Polymers and Oxidoreductases |
| title_sort | mass transfer in amperometric biosensors based on nanocomposite thin films of redox polymers and oxidoreductases |
| topic | Biosensor Redox Nanocomposite Hydrogel Enzyme |
| url | http://www.mdpi.com/1424-8220/2/3/79/ |
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