Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System
Background: In events such as a nuclear explosion or leakage of radioactive material from nuclear power dungeons or other events in nuclear medicine departments in hospitals, many people accidentally receive an unspecified amount of ionizing radiation. First step to treat is evaluation of radiation...
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| Format: | Article |
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Isfahan University of Medical Sciences
2014-07-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/3810 |
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| author | Mohammad Bagher Tavakkoli Majid Kheirollahi Ali Kiani Mohammad Kazemi Leili Mohebat Shagayegh Haghjooy Javanmard Mahnaz Roayaei |
| author_facet | Mohammad Bagher Tavakkoli Majid Kheirollahi Ali Kiani Mohammad Kazemi Leili Mohebat Shagayegh Haghjooy Javanmard Mahnaz Roayaei |
| author_sort | Mohammad Bagher Tavakkoli |
| collection | DOAJ |
| description | Background: In events such as a nuclear explosion or leakage of radioactive material from nuclear power dungeons or other events in nuclear medicine departments in hospitals, many people accidentally receive an unspecified amount of ionizing radiation. First step to treat is evaluation of radiation dose received by the victims. In such situation and in radiotherapy program, dosimetry is used for evaluation of treatment planning. Some measurable biological indexes used for evaluation of dose of radiation. Some measurable biological indexes can be used in biological dosimetry to measure the radiation dose and estimate the radiation effect.
Methods: In this study, the test for biological dosimetry based on apoptosis induced by gamma radiation in peripheral blood T-lymphocytes was performed in 16 volunteers. The blood lymphocytes were isolated and cultured in RPMI (Roswell Park Memorial Institute) 1640 medium and then, were placed in 5% CO2 atmosphere at 37°C. Then, the samples were prepared from the culture medium and radiated with different doses of gamma radiation. Sample transferred to incubator again to measure their apoptosis. Radiation-induced apoptosis in the cell population was measured by flow cytometry using Annexin V + fluorescein isothiocyanate (FITC) and prodidium iodide (PI) stains.
Findings: Radiation induced apoptosis was measureable with enough precision. But measured apoptosis depended on delay time after irradiation and protocole of flow cytometry.
Conclusion: The results of this study show that it is possible to use radiation for measuring apoptosis as a biological dosimeter in a short time after radiation exposure in the events such as a nuclear explosion or leakage of radioactive material. |
| first_indexed | 2024-03-12T08:45:06Z |
| format | Article |
| id | doaj.art-acf99bf0cd954413850a8c5e63210434 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:45:06Z |
| publishDate | 2014-07-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-acf99bf0cd954413850a8c5e632104342023-09-02T16:29:36ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2014-07-01322846106201593Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry SystemMohammad Bagher Tavakkoli0Majid Kheirollahi1Ali Kiani2Mohammad Kazemi3Leili Mohebat4Shagayegh Haghjooy Javanmard5Mahnaz Roayaei6Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan IranAssistant Professor, Pediatric Inherited Diseases Research Center AND Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranPhD Student, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences AND Lecturer, Department of Physics, School of Sciences, Malek-Ashtar University of Technology, Isfahan, IranPhD Student, Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranCentral Research Lab, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Oncology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: In events such as a nuclear explosion or leakage of radioactive material from nuclear power dungeons or other events in nuclear medicine departments in hospitals, many people accidentally receive an unspecified amount of ionizing radiation. First step to treat is evaluation of radiation dose received by the victims. In such situation and in radiotherapy program, dosimetry is used for evaluation of treatment planning. Some measurable biological indexes used for evaluation of dose of radiation. Some measurable biological indexes can be used in biological dosimetry to measure the radiation dose and estimate the radiation effect. Methods: In this study, the test for biological dosimetry based on apoptosis induced by gamma radiation in peripheral blood T-lymphocytes was performed in 16 volunteers. The blood lymphocytes were isolated and cultured in RPMI (Roswell Park Memorial Institute) 1640 medium and then, were placed in 5% CO2 atmosphere at 37°C. Then, the samples were prepared from the culture medium and radiated with different doses of gamma radiation. Sample transferred to incubator again to measure their apoptosis. Radiation-induced apoptosis in the cell population was measured by flow cytometry using Annexin V + fluorescein isothiocyanate (FITC) and prodidium iodide (PI) stains. Findings: Radiation induced apoptosis was measureable with enough precision. But measured apoptosis depended on delay time after irradiation and protocole of flow cytometry. Conclusion: The results of this study show that it is possible to use radiation for measuring apoptosis as a biological dosimeter in a short time after radiation exposure in the events such as a nuclear explosion or leakage of radioactive material.http://jims.mui.ac.ir/index.php/jims/article/view/3810ApoptosisFlowcytometryT-cellsLymphocyteBiological dosimetryRadiation ApoptosisBiological dosimetryT-cellsFlowcytometryLymphocyteRadiation |
| spellingShingle | Mohammad Bagher Tavakkoli Majid Kheirollahi Ali Kiani Mohammad Kazemi Leili Mohebat Shagayegh Haghjooy Javanmard Mahnaz Roayaei Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System مجله دانشکده پزشکی اصفهان Apoptosis Flowcytometry T-cells Lymphocyte Biological dosimetry Radiation Apoptosis Biological dosimetry T-cells Flowcytometry Lymphocyte Radiation |
| title | Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System |
| title_full | Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System |
| title_fullStr | Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System |
| title_full_unstemmed | Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System |
| title_short | Evaluation of Measuring Radiation-Induced Apoptosis in Human T-Lymphocytes by Flow Cytometry as a Biological Dosimetry System |
| title_sort | evaluation of measuring radiation induced apoptosis in human t lymphocytes by flow cytometry as a biological dosimetry system |
| topic | Apoptosis Flowcytometry T-cells Lymphocyte Biological dosimetry Radiation Apoptosis Biological dosimetry T-cells Flowcytometry Lymphocyte Radiation |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/3810 |
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