Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium
Study objective: to optimize diagnostic measures in patients with adenomyosis and/or hyperplastic endometrial processes (HEP). Material and methods. The study included 128 patients aged from 27 to 53 years. The first group included 38 (29.6%) patients with a grade I–II adenomyosis, the second group...
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| Format: | Article |
| Language: | English |
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Publishing House TRILIST
2021-05-01
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| Series: | Репродуктивная эндокринология |
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| Online Access: | http://reproduct-endo.com/article/view/232829 |
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| author | T.V. Mielikhova O.V. Gryshchenko V.V. Lazurenko R.A. Safonov O.S. Alekseeva |
| author_facet | T.V. Mielikhova O.V. Gryshchenko V.V. Lazurenko R.A. Safonov O.S. Alekseeva |
| author_sort | T.V. Mielikhova |
| collection | DOAJ |
| description | Study objective: to optimize diagnostic measures in patients with adenomyosis and/or hyperplastic endometrial processes (HEP).
Material and methods. The study included 128 patients aged from 27 to 53 years. The first group included 38 (29.6%) patients with a grade I–II adenomyosis, the second group included 41 (32.1%) patients with a combination of grade I–II adenomyosis and HEP and/or uterine leiomyoma, the third group included 39 (30.5%) women with only HEP, the fourth (control) group included 10 (7,8%) women without endometrial pathology. Patients underwent hysteroscopy with targeted biopsy, elucidated the pathomorphological features of connective tissue component of the endometrium and uterus transitional zone, studied specific markers of adenomyosis progression, namely vascular endothelial growth factor and Ki-67 proliferation index, and also determined the parameters of shear wave ultrasound elastography.
Results. Routine histological examination of adenomyosis was confirmed only in 11 (30.6%) cases out of 36 suspected, while HEP (polyps, glandular hyperplasia) and hyperplastic pathology of endocervix (polyps) – in 34 (97.1%) cases out of 35 suspected. Ki-67 antigen expression was positive in the nuclei of epithelial cells in the adenomyosis glands and in the cytogenic stroma cells. A greater value of expression was in the epithelium of glands in superficially located heterotopies – with grade I–II adenomyosis compared with other studied groups. Shear wave elastography showed significant differences in the myometrium color between the first, third and control groups. The unchanged myometrium in all patients of the control group was characterized by staining in shades of blue, while in 9 (90%) patients the color was uniform.
Conclusion. It is recommended to use a shear wave ultrasound elastography in laboratory and instrumental examination with determination of Ki-67 proliferation protein expression and intensification of the neovascularization process in endometrial biopsy specimens to improve the diagnosis of adenomyosis. |
| first_indexed | 2024-12-16T08:35:25Z |
| format | Article |
| id | doaj.art-ad00fb4e28674976994675df5630d376 |
| institution | Directory Open Access Journal |
| issn | 2309-4117 2411-1295 |
| language | English |
| last_indexed | 2024-12-16T08:35:25Z |
| publishDate | 2021-05-01 |
| publisher | Publishing House TRILIST |
| record_format | Article |
| series | Репродуктивная эндокринология |
| spelling | doaj.art-ad00fb4e28674976994675df5630d3762022-12-21T22:37:47ZengPublishing House TRILISTРепродуктивная эндокринология2309-41172411-12952021-05-0158343910.18370/2309-4117.2021.58.34-39270462Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometriumT.V. Mielikhova0https://orcid.org/0000-0001-7594-6544O.V. Gryshchenko1https://orcid.org/0000-0002-8485-7022V.V. Lazurenko2https://orcid.org/0000-0002-3252-4947R.A. Safonov3https://orcid.org/0000-0002-3876-4415O.S. Alekseeva4https://orcid.org/0000-0002-6722-5509Kharkiv National Medical UniversityKharkiv Medical Academy of Postgraduate EducationKharkiv National Medical UniversityKharkiv National Medical University; Kharkiv Regional Clinical HospitalKharkiv National Medical UniversityStudy objective: to optimize diagnostic measures in patients with adenomyosis and/or hyperplastic endometrial processes (HEP). Material and methods. The study included 128 patients aged from 27 to 53 years. The first group included 38 (29.6%) patients with a grade I–II adenomyosis, the second group included 41 (32.1%) patients with a combination of grade I–II adenomyosis and HEP and/or uterine leiomyoma, the third group included 39 (30.5%) women with only HEP, the fourth (control) group included 10 (7,8%) women without endometrial pathology. Patients underwent hysteroscopy with targeted biopsy, elucidated the pathomorphological features of connective tissue component of the endometrium and uterus transitional zone, studied specific markers of adenomyosis progression, namely vascular endothelial growth factor and Ki-67 proliferation index, and also determined the parameters of shear wave ultrasound elastography. Results. Routine histological examination of adenomyosis was confirmed only in 11 (30.6%) cases out of 36 suspected, while HEP (polyps, glandular hyperplasia) and hyperplastic pathology of endocervix (polyps) – in 34 (97.1%) cases out of 35 suspected. Ki-67 antigen expression was positive in the nuclei of epithelial cells in the adenomyosis glands and in the cytogenic stroma cells. A greater value of expression was in the epithelium of glands in superficially located heterotopies – with grade I–II adenomyosis compared with other studied groups. Shear wave elastography showed significant differences in the myometrium color between the first, third and control groups. The unchanged myometrium in all patients of the control group was characterized by staining in shades of blue, while in 9 (90%) patients the color was uniform. Conclusion. It is recommended to use a shear wave ultrasound elastography in laboratory and instrumental examination with determination of Ki-67 proliferation protein expression and intensification of the neovascularization process in endometrial biopsy specimens to improve the diagnosis of adenomyosis.http://reproduct-endo.com/article/view/232829adenomyosisendometriosisimmunohistochemical studyki-67shear wave elastography |
| spellingShingle | T.V. Mielikhova O.V. Gryshchenko V.V. Lazurenko R.A. Safonov O.S. Alekseeva Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium Репродуктивная эндокринология adenomyosis endometriosis immunohistochemical study ki-67 shear wave elastography |
| title | Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium |
| title_full | Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium |
| title_fullStr | Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium |
| title_full_unstemmed | Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium |
| title_short | Optimization of diagnostic studies in patients with adenomiosis and/or hyperplastic processes of endometrium |
| title_sort | optimization of diagnostic studies in patients with adenomiosis and or hyperplastic processes of endometrium |
| topic | adenomyosis endometriosis immunohistochemical study ki-67 shear wave elastography |
| url | http://reproduct-endo.com/article/view/232829 |
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