Sample preparation for mass spectrometry imaging of plant tissues: a review
Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining steps. These advantages make it an attracti...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00060/full |
| _version_ | 1819207241995124736 |
|---|---|
| author | Yonghui eDong Yonghui eDong Bin eLi Sergey eMalitsky Ilana eRogachev Asaph eAharoni Filip eKaftan Aleš eSvatoš Pietro eFranceschi |
| author_facet | Yonghui eDong Yonghui eDong Bin eLi Sergey eMalitsky Ilana eRogachev Asaph eAharoni Filip eKaftan Aleš eSvatoš Pietro eFranceschi |
| author_sort | Yonghui eDong |
| collection | DOAJ |
| description | Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining steps. These advantages make it an attractive molecular histology tool in medical, pharmaceutical and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods specific for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of various MSI techniques in plants are reviewed according to different classes of plant metabolites. |
| first_indexed | 2024-12-23T05:20:22Z |
| format | Article |
| id | doaj.art-ad08a3b3d19b4cfc8ce00473ae718999 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T05:20:22Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ad08a3b3d19b4cfc8ce00473ae7189992022-12-21T17:58:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00060170331Sample preparation for mass spectrometry imaging of plant tissues: a reviewYonghui eDong0Yonghui eDong1Bin eLi2Sergey eMalitsky3Ilana eRogachev4Asaph eAharoni5Filip eKaftan6Aleš eSvatoš7Pietro eFranceschi8Weizmann Institute of ScienceResearch and Innovation Centre - Fondazione Edmund MachUniversity of Illinois at Urbana-ChampaignWeizmann Institute of ScienceWeizmann Institute of ScienceWeizmann Institute of ScienceMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyResearch and Innovation Centre - Fondazione Edmund MachMass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining steps. These advantages make it an attractive molecular histology tool in medical, pharmaceutical and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods specific for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of various MSI techniques in plants are reviewed according to different classes of plant metabolites.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00060/fullSIMSMALDIMass spectrometry imagingplant metabolitesDesiMolecular histology |
| spellingShingle | Yonghui eDong Yonghui eDong Bin eLi Sergey eMalitsky Ilana eRogachev Asaph eAharoni Filip eKaftan Aleš eSvatoš Pietro eFranceschi Sample preparation for mass spectrometry imaging of plant tissues: a review Frontiers in Plant Science SIMS MALDI Mass spectrometry imaging plant metabolites Desi Molecular histology |
| title | Sample preparation for mass spectrometry imaging of plant tissues: a review |
| title_full | Sample preparation for mass spectrometry imaging of plant tissues: a review |
| title_fullStr | Sample preparation for mass spectrometry imaging of plant tissues: a review |
| title_full_unstemmed | Sample preparation for mass spectrometry imaging of plant tissues: a review |
| title_short | Sample preparation for mass spectrometry imaging of plant tissues: a review |
| title_sort | sample preparation for mass spectrometry imaging of plant tissues a review |
| topic | SIMS MALDI Mass spectrometry imaging plant metabolites Desi Molecular histology |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00060/full |
| work_keys_str_mv | AT yonghuiedong samplepreparationformassspectrometryimagingofplanttissuesareview AT yonghuiedong samplepreparationformassspectrometryimagingofplanttissuesareview AT bineli samplepreparationformassspectrometryimagingofplanttissuesareview AT sergeyemalitsky samplepreparationformassspectrometryimagingofplanttissuesareview AT ilanaerogachev samplepreparationformassspectrometryimagingofplanttissuesareview AT asapheaharoni samplepreparationformassspectrometryimagingofplanttissuesareview AT filipekaftan samplepreparationformassspectrometryimagingofplanttissuesareview AT alesesvatos samplepreparationformassspectrometryimagingofplanttissuesareview AT pietroefranceschi samplepreparationformassspectrometryimagingofplanttissuesareview |