GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair
Promising wound dressings can achieve rapid soft-tissue filling while refactoring the biochemical and biophysical microenvironment to recruit endogenous cells, facilitating tissue healing, integration, and regeneration. In this study, a tissue biomolecule-responsive hydrogel matrix, employing natura...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/1/56 |
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| author | Hongdou Shen Pei Wang Xiaoke Han Mengli Ma Yinghui Shang Ye Ju Saiji Shen Feng Yin Qigang Wang |
| author_facet | Hongdou Shen Pei Wang Xiaoke Han Mengli Ma Yinghui Shang Ye Ju Saiji Shen Feng Yin Qigang Wang |
| author_sort | Hongdou Shen |
| collection | DOAJ |
| description | Promising wound dressings can achieve rapid soft-tissue filling while refactoring the biochemical and biophysical microenvironment to recruit endogenous cells, facilitating tissue healing, integration, and regeneration. In this study, a tissue biomolecule-responsive hydrogel matrix, employing natural silk fibroin (SF) as a functional biopolymer and haemoglobin (Hb) as a peroxidase-like biocatalyst, was fabricated through cascade enzymatic crosslinking. The hydrogels possessed mechanical tunability and displayed adjustable gelation times. A tyrosine unit on SF stabilised the structure of Hb during the cascade oxidation process; thus, the immobilized Hb in SF hydrogels exhibited higher biocatalytic efficiency than the free enzyme system, which provided a continuously antioxidative system. The regulation of the dual enzyme ratio endowed the hydrogels with favourable biocompatibility, biodegradability, and adhesion strength. These multifunctional hydrogels provided a three-dimensional porous extracellular matrix-like microenvironment for promoting cell adhesion and proliferation. A rat model with a full-thickness skin defect revealed accelerated wound regeneration via collagen deposition, re-epithelialisation and revascularisation. Enzyme-loaded hydrogels are an attractive and high-safety biofilling material with the potential for wound healing, tissue regeneration, and haemostasis. |
| first_indexed | 2024-03-10T01:26:08Z |
| format | Article |
| id | doaj.art-ad0b3a74473c49c7959e0597e46b9a96 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T01:26:08Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Gels |
| spelling | doaj.art-ad0b3a74473c49c7959e0597e46b9a962023-11-23T13:50:35ZengMDPI AGGels2310-28612022-01-01815610.3390/gels8010056GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound RepairHongdou Shen0Pei Wang1Xiaoke Han2Mengli Ma3Yinghui Shang4Ye Ju5Saiji Shen6Feng Yin7Qigang Wang8Department of Joint Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaDepartment of Joint Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaDepartment of Joint Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaSchool of Chemical Science and Engineering, Tongji University, Shanghai 200092, ChinaPromising wound dressings can achieve rapid soft-tissue filling while refactoring the biochemical and biophysical microenvironment to recruit endogenous cells, facilitating tissue healing, integration, and regeneration. In this study, a tissue biomolecule-responsive hydrogel matrix, employing natural silk fibroin (SF) as a functional biopolymer and haemoglobin (Hb) as a peroxidase-like biocatalyst, was fabricated through cascade enzymatic crosslinking. The hydrogels possessed mechanical tunability and displayed adjustable gelation times. A tyrosine unit on SF stabilised the structure of Hb during the cascade oxidation process; thus, the immobilized Hb in SF hydrogels exhibited higher biocatalytic efficiency than the free enzyme system, which provided a continuously antioxidative system. The regulation of the dual enzyme ratio endowed the hydrogels with favourable biocompatibility, biodegradability, and adhesion strength. These multifunctional hydrogels provided a three-dimensional porous extracellular matrix-like microenvironment for promoting cell adhesion and proliferation. A rat model with a full-thickness skin defect revealed accelerated wound regeneration via collagen deposition, re-epithelialisation and revascularisation. Enzyme-loaded hydrogels are an attractive and high-safety biofilling material with the potential for wound healing, tissue regeneration, and haemostasis.https://www.mdpi.com/2310-2861/8/1/56silk fibroinantioxidative hydrogelscascade oxidized crosslinkingtissue-responsivewound regeneration |
| spellingShingle | Hongdou Shen Pei Wang Xiaoke Han Mengli Ma Yinghui Shang Ye Ju Saiji Shen Feng Yin Qigang Wang GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair Gels silk fibroin antioxidative hydrogels cascade oxidized crosslinking tissue-responsive wound regeneration |
| title | GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair |
| title_full | GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair |
| title_fullStr | GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair |
| title_full_unstemmed | GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair |
| title_short | GOx/Hb Cascade Oxidized Crosslinking of Silk Fibroin for Tissue-Responsive Wound Repair |
| title_sort | gox hb cascade oxidized crosslinking of silk fibroin for tissue responsive wound repair |
| topic | silk fibroin antioxidative hydrogels cascade oxidized crosslinking tissue-responsive wound regeneration |
| url | https://www.mdpi.com/2310-2861/8/1/56 |
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