Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/12/2/37 |
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| author | Katarzyna Pieklarz Grzegorz Galita Michał Tylman Waldemar Maniukiewicz Ewa Kucharska Ireneusz Majsterek Zofia Modrzejewska |
| author_facet | Katarzyna Pieklarz Grzegorz Galita Michał Tylman Waldemar Maniukiewicz Ewa Kucharska Ireneusz Majsterek Zofia Modrzejewska |
| author_sort | Katarzyna Pieklarz |
| collection | DOAJ |
| description | Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering. |
| first_indexed | 2024-03-10T11:14:28Z |
| format | Article |
| id | doaj.art-ad0c5936bcf94c3791b4f503e77f7159 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T11:14:28Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-ad0c5936bcf94c3791b4f503e77f71592023-11-21T20:32:25ZengMDPI AGJournal of Functional Biomaterials2079-49832021-05-011223710.3390/jfb12020037Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering ApplicationKatarzyna Pieklarz0Grzegorz Galita1Michał Tylman2Waldemar Maniukiewicz3Ewa Kucharska4Ireneusz Majsterek5Zofia Modrzejewska6Department of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, PolandDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, Narutowicza 60 Street, 90-136 Lodz, PolandPGE Gornictwo i Energetyka Konwencjonalna S.A., Weglowa 5 Street, 97-400 Belchatow, PolandInstitute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116 Street, 90-924 Lodz, PolandDepartment of Gerontology, Geriatrics and Social Work, Jesuit University Ignatianum in Krakow, Kopernika 26 Street, 31-501 Krakow, PolandDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, Narutowicza 60 Street, 90-136 Lodz, PolandDepartment of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, PolandRecently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering.https://www.mdpi.com/2079-4983/12/2/37tissue engineeringnatural polymerchitosanthermosensitive hydrogelstructural propertiescytotoxicity |
| spellingShingle | Katarzyna Pieklarz Grzegorz Galita Michał Tylman Waldemar Maniukiewicz Ewa Kucharska Ireneusz Majsterek Zofia Modrzejewska Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application Journal of Functional Biomaterials tissue engineering natural polymer chitosan thermosensitive hydrogel structural properties cytotoxicity |
| title | Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application |
| title_full | Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application |
| title_fullStr | Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application |
| title_full_unstemmed | Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application |
| title_short | Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application |
| title_sort | physico chemical properties and biocompatibility of thermosensitive chitosan lactate and chitosan chloride hydrogels developed for tissue engineering application |
| topic | tissue engineering natural polymer chitosan thermosensitive hydrogel structural properties cytotoxicity |
| url | https://www.mdpi.com/2079-4983/12/2/37 |
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