Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin
Infected bone defects (IBDs) remains a challenging problem for orthopedists. Clinically, routine management for IBDs has two stages: debridement and systematic antibiotics administration to control infection, and secondary grafting to repair bone defects. Whereas the efficacy is not satisfactory, be...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-06-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22005011 |
| _version_ | 1797866111748800512 |
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| author | Hu Qian Ting Lei Long Hua Yu Zhang Dongyu Wang Jiangyu Nan Wenbin Liu Yan Sun Yihe Hu Pengfei Lei |
| author_facet | Hu Qian Ting Lei Long Hua Yu Zhang Dongyu Wang Jiangyu Nan Wenbin Liu Yan Sun Yihe Hu Pengfei Lei |
| author_sort | Hu Qian |
| collection | DOAJ |
| description | Infected bone defects (IBDs) remains a challenging problem for orthopedists. Clinically, routine management for IBDs has two stages: debridement and systematic antibiotics administration to control infection, and secondary grafting to repair bone defects. Whereas the efficacy is not satisfactory, because the overuse of antibiotics may lead to systemic toxicity, and the emergence of drug-resistant bacteria, as well as the secondary surgery would cause additional trauma and economic burden to the patients. Therefore, it is imperative to develop a novel scaffold for one-stage repair of IBDs. In this study, vancomycin (Van) was encapsulated into poly(lactic co-glycolic acid) (PLGA) microspheres through the double emulsion method, which were then loaded into the additively-manufactured porous tantalum (AM-Ta) through gelatin methacryloyl (GelMA) hydrogel to produce the composite Ta/GelMA hydrogel (Gel)/PLGA/vancomycin(Van) scaffolds for repairing IBDs. Physiochemical characterization of the newly-developed scaffold indicated that the releasing duration of Van was over 2 weeks. Biological experiments indicated good biocompatibility of the composite scaffold, as well as bacteriostasis and osteointegration properties, which showed great potential for clinical application. The construction of this novel scaffold would provide new sight into the development of orthopaedic implants, shedding a novel light on the treatment of IBDs. |
| first_indexed | 2024-04-09T23:18:48Z |
| format | Article |
| id | doaj.art-ad0dade4c578482b803a6aba8e12a9d7 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-09T23:18:48Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-ad0dade4c578482b803a6aba8e12a9d72023-03-22T04:37:31ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-06-0124450462Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycinHu Qian0Ting Lei1Long Hua2Yu Zhang3Dongyu Wang4Jiangyu Nan5Wenbin Liu6Yan Sun7Yihe Hu8Pengfei Lei9Department of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, China; Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China; Xiangya School of Medicine, Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, ChinaDepartment of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, China; Department of Orthopedic Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, China; Corresponding author. Department of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, China.Department of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, China; Department of Orthopedic Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, China; Corresponding author. Department of Orthopedic Surgery, Xiangya Hospital Central South University, Changsha, 410008, China.Infected bone defects (IBDs) remains a challenging problem for orthopedists. Clinically, routine management for IBDs has two stages: debridement and systematic antibiotics administration to control infection, and secondary grafting to repair bone defects. Whereas the efficacy is not satisfactory, because the overuse of antibiotics may lead to systemic toxicity, and the emergence of drug-resistant bacteria, as well as the secondary surgery would cause additional trauma and economic burden to the patients. Therefore, it is imperative to develop a novel scaffold for one-stage repair of IBDs. In this study, vancomycin (Van) was encapsulated into poly(lactic co-glycolic acid) (PLGA) microspheres through the double emulsion method, which were then loaded into the additively-manufactured porous tantalum (AM-Ta) through gelatin methacryloyl (GelMA) hydrogel to produce the composite Ta/GelMA hydrogel (Gel)/PLGA/vancomycin(Van) scaffolds for repairing IBDs. Physiochemical characterization of the newly-developed scaffold indicated that the releasing duration of Van was over 2 weeks. Biological experiments indicated good biocompatibility of the composite scaffold, as well as bacteriostasis and osteointegration properties, which showed great potential for clinical application. The construction of this novel scaffold would provide new sight into the development of orthopaedic implants, shedding a novel light on the treatment of IBDs.http://www.sciencedirect.com/science/article/pii/S2452199X22005011Infected bone defectsPorous tantalumPLGA microspheresVancomycin |
| spellingShingle | Hu Qian Ting Lei Long Hua Yu Zhang Dongyu Wang Jiangyu Nan Wenbin Liu Yan Sun Yihe Hu Pengfei Lei Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin Bioactive Materials Infected bone defects Porous tantalum PLGA microspheres Vancomycin |
| title | Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin |
| title_full | Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin |
| title_fullStr | Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin |
| title_full_unstemmed | Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin |
| title_short | Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin |
| title_sort | fabrication bacteriostasis and osteointegration properties researches of the additively manufactured porous tantalum scaffolds loading vancomycin |
| topic | Infected bone defects Porous tantalum PLGA microspheres Vancomycin |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22005011 |
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