Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap)
Background Affinity peptides, as a core part of affinity chromatography, play an important role in the purification of target molecules. Methods Here we describe the use of molecular docking technology for virtual screening of affinity peptides that specifically recognize the PCV2 Cap protein for th...
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PeerJ Inc.
2019-12-01
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| Online Access: | https://peerj.com/articles/8132.pdf |
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| author | Junfang Hao Fangyu Wang Guangxu Xing Yunchao Liu Ruiguang Deng Hao Zhang Anchun Cheng Gaiping Zhang |
| author_facet | Junfang Hao Fangyu Wang Guangxu Xing Yunchao Liu Ruiguang Deng Hao Zhang Anchun Cheng Gaiping Zhang |
| author_sort | Junfang Hao |
| collection | DOAJ |
| description | Background Affinity peptides, as a core part of affinity chromatography, play an important role in the purification of target molecules. Methods Here we describe the use of molecular docking technology for virtual screening of affinity peptides that specifically recognize the PCV2 Cap protein for the first time. Thirteen candidate peptides with high scores were obtained and then further characterized. Experimentally, the affinity and sensitivity of the peptides studied were identified by ELISA and LSPR, respectively. In order to investigate the purification effect of a selected peptide (L11) for the recombinant PCV2 Cap protein, it was coupled to NHS agarose magnetic beads as an affinity adsorbent (NaMB-L11); and the ligand density of the affinity adsorbent and pH value in the purification of the recombinant PCV2 Cap protein were optimized. Results Our data showed that the peptide L11- DYWWQSWE has the smallest KD = 103 nM with higher specificity for PCV2 Cap protein recognition. The NaMB-L11 affinity adsorbent yielded a purified Cap sample with 98% purity at 90% recovery in a single step. Conclusion Based on the structure, we obtained a high affinity peptide L11 binding to the PCV2 Cap protein by molecular docking technology. It not only provides a theoretical basis for the design of PCV2 Cap affinity peptide, but a new method for the purification of the PCV2 Cap protein. |
| first_indexed | 2024-03-09T07:53:50Z |
| format | Article |
| id | doaj.art-ad0e6e5c00ef4a75b828768939a194ee |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:53:50Z |
| publishDate | 2019-12-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-ad0e6e5c00ef4a75b828768939a194ee2023-12-03T01:22:20ZengPeerJ Inc.PeerJ2167-83592019-12-017e813210.7717/peerj.8132Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap)Junfang Hao0Fangyu Wang1Guangxu Xing2Yunchao Liu3Ruiguang Deng4Hao Zhang5Anchun Cheng6Gaiping Zhang7Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaHenan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaBackground Affinity peptides, as a core part of affinity chromatography, play an important role in the purification of target molecules. Methods Here we describe the use of molecular docking technology for virtual screening of affinity peptides that specifically recognize the PCV2 Cap protein for the first time. Thirteen candidate peptides with high scores were obtained and then further characterized. Experimentally, the affinity and sensitivity of the peptides studied were identified by ELISA and LSPR, respectively. In order to investigate the purification effect of a selected peptide (L11) for the recombinant PCV2 Cap protein, it was coupled to NHS agarose magnetic beads as an affinity adsorbent (NaMB-L11); and the ligand density of the affinity adsorbent and pH value in the purification of the recombinant PCV2 Cap protein were optimized. Results Our data showed that the peptide L11- DYWWQSWE has the smallest KD = 103 nM with higher specificity for PCV2 Cap protein recognition. The NaMB-L11 affinity adsorbent yielded a purified Cap sample with 98% purity at 90% recovery in a single step. Conclusion Based on the structure, we obtained a high affinity peptide L11 binding to the PCV2 Cap protein by molecular docking technology. It not only provides a theoretical basis for the design of PCV2 Cap affinity peptide, but a new method for the purification of the PCV2 Cap protein.https://peerj.com/articles/8132.pdfAffinity peptidesPCV2 CapMolecular docking virtual screeningPurification |
| spellingShingle | Junfang Hao Fangyu Wang Guangxu Xing Yunchao Liu Ruiguang Deng Hao Zhang Anchun Cheng Gaiping Zhang Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) PeerJ Affinity peptides PCV2 Cap Molecular docking virtual screening Purification |
| title | Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) |
| title_full | Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) |
| title_fullStr | Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) |
| title_full_unstemmed | Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) |
| title_short | Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II Capsid (PCV2 Cap) |
| title_sort | design and preliminary application of affinity peptide based on the structure of the porcine circovirus type ii capsid pcv2 cap |
| topic | Affinity peptides PCV2 Cap Molecular docking virtual screening Purification |
| url | https://peerj.com/articles/8132.pdf |
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