Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis.
Nuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the mi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4830538?pdf=render |
| _version_ | 1819048358949421056 |
|---|---|
| author | Kimberly N Weiderhold Maria Fadri-Moskwik Jing Pan Michiya Nishino Carol Chuang Arpaporn Deeraksa Sue-Hwa Lin Li-Yuan Yu-Lee |
| author_facet | Kimberly N Weiderhold Maria Fadri-Moskwik Jing Pan Michiya Nishino Carol Chuang Arpaporn Deeraksa Sue-Hwa Lin Li-Yuan Yu-Lee |
| author_sort | Kimberly N Weiderhold |
| collection | DOAJ |
| description | Nuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. Inhibition of Aurora B by ZM447439 reduced NudC phosphorylation, suggesting that NudC is an Aurora B substrate in vivo. We identified T40 on NudC as an Aurora B phosphorylation site. NudC depletion resulted in cytokinesis failure with a dramatic elongation of the intercellular bridge between daughter cells, sustained Aurora B activity at the midbody, and reduced cell abscission. These cytokinetic defects can be rescued by the ectopic expression of wild-type NudC. Reconstitution with T40A phospho-defective NudC was found to rescue the cytokinesis defect. In contrast, reconstitution with the T40D phospho-mimetic NudC was inefficient in supporting the completion of cytokinesis. These results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis. |
| first_indexed | 2024-12-21T11:15:00Z |
| format | Article |
| id | doaj.art-ad0f0c976dce462aaea219b2306c5a0c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T11:15:00Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad0f0c976dce462aaea219b2306c5a0c2022-12-21T19:05:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015345510.1371/journal.pone.0153455Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis.Kimberly N WeiderholdMaria Fadri-MoskwikJing PanMichiya NishinoCarol ChuangArpaporn DeeraksaSue-Hwa LinLi-Yuan Yu-LeeNuclear distribution protein C (NudC) is a mitotic regulator that plays a role in cytokinesis. However, how NudC is regulated during cytokinesis remains unclear. Here, we show that NudC is phosphorylated by Aurora B, a kinase critical for cell abscission. NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. Inhibition of Aurora B by ZM447439 reduced NudC phosphorylation, suggesting that NudC is an Aurora B substrate in vivo. We identified T40 on NudC as an Aurora B phosphorylation site. NudC depletion resulted in cytokinesis failure with a dramatic elongation of the intercellular bridge between daughter cells, sustained Aurora B activity at the midbody, and reduced cell abscission. These cytokinetic defects can be rescued by the ectopic expression of wild-type NudC. Reconstitution with T40A phospho-defective NudC was found to rescue the cytokinesis defect. In contrast, reconstitution with the T40D phospho-mimetic NudC was inefficient in supporting the completion of cytokinesis. These results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis.http://europepmc.org/articles/PMC4830538?pdf=render |
| spellingShingle | Kimberly N Weiderhold Maria Fadri-Moskwik Jing Pan Michiya Nishino Carol Chuang Arpaporn Deeraksa Sue-Hwa Lin Li-Yuan Yu-Lee Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. PLoS ONE |
| title | Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. |
| title_full | Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. |
| title_fullStr | Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. |
| title_full_unstemmed | Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. |
| title_short | Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis. |
| title_sort | dynamic phosphorylation of nudc by aurora b in cytokinesis |
| url | http://europepmc.org/articles/PMC4830538?pdf=render |
| work_keys_str_mv | AT kimberlynweiderhold dynamicphosphorylationofnudcbyaurorabincytokinesis AT mariafadrimoskwik dynamicphosphorylationofnudcbyaurorabincytokinesis AT jingpan dynamicphosphorylationofnudcbyaurorabincytokinesis AT michiyanishino dynamicphosphorylationofnudcbyaurorabincytokinesis AT carolchuang dynamicphosphorylationofnudcbyaurorabincytokinesis AT arpaporndeeraksa dynamicphosphorylationofnudcbyaurorabincytokinesis AT suehwalin dynamicphosphorylationofnudcbyaurorabincytokinesis AT liyuanyulee dynamicphosphorylationofnudcbyaurorabincytokinesis |