Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars
Abstract Tissue bioengineering has been applied to Endodontics to seek a more biological treatment. The presence of blood vessels is crucial for cell nutrition during tissue formation. Objective This study analysed the application of vascular endothelial growth factor (VEGF) in the angiogenesis of...
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| Format: | Article |
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University of São Paulo
2018-05-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100457&lng=en&tlng=en |
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| author | Cleber Keiti Nabeshima José Edgar Valdivia Hector Caballero-Flores Victor Elias Arana-Chavez Manoel Eduardo de Lima Machado |
| author_facet | Cleber Keiti Nabeshima José Edgar Valdivia Hector Caballero-Flores Victor Elias Arana-Chavez Manoel Eduardo de Lima Machado |
| author_sort | Cleber Keiti Nabeshima |
| collection | DOAJ |
| description | Abstract Tissue bioengineering has been applied to Endodontics to seek a more biological treatment. The presence of blood vessels is crucial for cell nutrition during tissue formation. Objective This study analysed the application of vascular endothelial growth factor (VEGF) in the angiogenesis of mature root canals. Material and methods Upper first molars of twelve 13-week old Wistar male rats were used. The root pulp of the mesiobuccal canal was removed and the root canal instrumented with K-files up to size #25. Periapical bleeding was induced into the root canal by introducing a #15 K-file beyond the apex. The teeth on the right side of the arch were filled up with blood clot (G1), whereas those on the left side were filled up with blood clot plus 50 ng/ml of VEGF (G2). Teeth were sealed with light-curing glass-ionomer cement and the animals were sacrificed after 60 days. The maxilla was dissected and fixed before obtaining serial sections for histological processing with haematoxylin-eosin (HE) and immunohistochemical factor-VIII. Immunohistochemical labelling was evaluated using scores for statistical analysis. Results Immunohistological analysis demonstrated the presence of angiogenesis in both groups, but with higher angiogenic maturation in G2 during the experimental period (p<0.05). HE staining showed connective tissue with absence of odontoblasts in all specimens. Conclusions It can be concluded that it is possible to obtain angiogenesis in mature root canals with or without the use of VEGF, although the latter tends to accelerate blood vessel formation. |
| first_indexed | 2024-12-22T19:57:37Z |
| format | Article |
| id | doaj.art-ad10672c5e49444a8f69f7506aa78c69 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-22T19:57:37Z |
| publishDate | 2018-05-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-ad10672c5e49444a8f69f7506aa78c692022-12-21T18:14:23ZengUniversity of São PauloJournal of Applied Oral Science1678-77652018-05-0126010.1590/1678-7757-2017-0437S1678-77572018000100457Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molarsCleber Keiti NabeshimaJosé Edgar ValdiviaHector Caballero-FloresVictor Elias Arana-ChavezManoel Eduardo de Lima MachadoAbstract Tissue bioengineering has been applied to Endodontics to seek a more biological treatment. The presence of blood vessels is crucial for cell nutrition during tissue formation. Objective This study analysed the application of vascular endothelial growth factor (VEGF) in the angiogenesis of mature root canals. Material and methods Upper first molars of twelve 13-week old Wistar male rats were used. The root pulp of the mesiobuccal canal was removed and the root canal instrumented with K-files up to size #25. Periapical bleeding was induced into the root canal by introducing a #15 K-file beyond the apex. The teeth on the right side of the arch were filled up with blood clot (G1), whereas those on the left side were filled up with blood clot plus 50 ng/ml of VEGF (G2). Teeth were sealed with light-curing glass-ionomer cement and the animals were sacrificed after 60 days. The maxilla was dissected and fixed before obtaining serial sections for histological processing with haematoxylin-eosin (HE) and immunohistochemical factor-VIII. Immunohistochemical labelling was evaluated using scores for statistical analysis. Results Immunohistological analysis demonstrated the presence of angiogenesis in both groups, but with higher angiogenic maturation in G2 during the experimental period (p<0.05). HE staining showed connective tissue with absence of odontoblasts in all specimens. Conclusions It can be concluded that it is possible to obtain angiogenesis in mature root canals with or without the use of VEGF, although the latter tends to accelerate blood vessel formation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100457&lng=en&tlng=enAngiogenesisVascular endothelial growth factorEndodontics |
| spellingShingle | Cleber Keiti Nabeshima José Edgar Valdivia Hector Caballero-Flores Victor Elias Arana-Chavez Manoel Eduardo de Lima Machado Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars Journal of Applied Oral Science Angiogenesis Vascular endothelial growth factor Endodontics |
| title | Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars |
| title_full | Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars |
| title_fullStr | Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars |
| title_full_unstemmed | Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars |
| title_short | Immunohistological study of the effect of vascular Endothelial Growth Factor on the angiogenesis of mature root canals in rat molars |
| title_sort | immunohistological study of the effect of vascular endothelial growth factor on the angiogenesis of mature root canals in rat molars |
| topic | Angiogenesis Vascular endothelial growth factor Endodontics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100457&lng=en&tlng=en |
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