The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen
Abstract Background Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and proteome. At the same time, pollen development and viability is largely sensitive to stress, particularly to elevated temperatures. The transcriptomic and proteomic changes du...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4824-5 |
| _version_ | 1819160318893359104 |
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| author | Mario Keller SPOT-ITN Consortium Stefan Simm |
| author_facet | Mario Keller SPOT-ITN Consortium Stefan Simm |
| author_sort | Mario Keller |
| collection | DOAJ |
| description | Abstract Background Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and proteome. At the same time, pollen development and viability is largely sensitive to stress, particularly to elevated temperatures. The transcriptomic and proteomic changes during pollen development and of different stages in response to elevated temperature was targeted to define the underlying molecular principles. Results The analysis of the transcriptome and proteome of Solanum lycopersicum pollen at tetrad, post-meiotic and mature stage before and after heat stress yielded a decline of the transcriptome but an increase of the proteome size throughout pollen development. Comparison of the transcriptome and proteome led to the discovery of two modes defined as direct and delayed translation. Here, genes of distinct functional processes are under the control of direct and delayed translation. The response of pollen to elevated temperature occurs rather at proteome, but not as drastic at the transcriptome level. Heat shock proteins, proteasome subunits, ribosomal proteins and eukaryotic initiation factors are most affected. On the example of heat shock proteins we demonstrate a decoupling of transcript and protein levels as well as a distinct regulation between the developmental stages. Conclusions The transcriptome and proteome of developing pollen undergo drastic changes in composition and quantity. Changes at the proteome level are a result of two modes assigned as direct and delayed translation. The response of pollen to elevated temperature is mainly regulated at the proteome level, whereby proteins related to synthesis and degradation of proteins are most responsive and might play a central role in the heat stress response of pollen. |
| first_indexed | 2024-12-22T16:54:33Z |
| format | Article |
| id | doaj.art-ad10bc7a9bc84600bbe321035a35e2f0 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T16:54:33Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-ad10bc7a9bc84600bbe321035a35e2f02022-12-21T18:19:30ZengBMCBMC Genomics1471-21642018-06-0119112010.1186/s12864-018-4824-5The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollenMario Keller0SPOT-ITN Consortium1Stefan Simm2Department of Biosciences, Molecular Cell Biology of Plants, Goethe UniversitySolanaceae Pollen Thermotolerance Initial Training Network ConsortiumDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe UniversityAbstract Background Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and proteome. At the same time, pollen development and viability is largely sensitive to stress, particularly to elevated temperatures. The transcriptomic and proteomic changes during pollen development and of different stages in response to elevated temperature was targeted to define the underlying molecular principles. Results The analysis of the transcriptome and proteome of Solanum lycopersicum pollen at tetrad, post-meiotic and mature stage before and after heat stress yielded a decline of the transcriptome but an increase of the proteome size throughout pollen development. Comparison of the transcriptome and proteome led to the discovery of two modes defined as direct and delayed translation. Here, genes of distinct functional processes are under the control of direct and delayed translation. The response of pollen to elevated temperature occurs rather at proteome, but not as drastic at the transcriptome level. Heat shock proteins, proteasome subunits, ribosomal proteins and eukaryotic initiation factors are most affected. On the example of heat shock proteins we demonstrate a decoupling of transcript and protein levels as well as a distinct regulation between the developmental stages. Conclusions The transcriptome and proteome of developing pollen undergo drastic changes in composition and quantity. Changes at the proteome level are a result of two modes assigned as direct and delayed translation. The response of pollen to elevated temperature is mainly regulated at the proteome level, whereby proteins related to synthesis and degradation of proteins are most responsive and might play a central role in the heat stress response of pollen.http://link.springer.com/article/10.1186/s12864-018-4824-5TranscriptomicsProteomicsPollen developmentHeat stress |
| spellingShingle | Mario Keller SPOT-ITN Consortium Stefan Simm The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen BMC Genomics Transcriptomics Proteomics Pollen development Heat stress |
| title | The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| title_full | The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| title_fullStr | The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| title_full_unstemmed | The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| title_short | The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| title_sort | coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen |
| topic | Transcriptomics Proteomics Pollen development Heat stress |
| url | http://link.springer.com/article/10.1186/s12864-018-4824-5 |
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