Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta
The potential of microorganisms for the treatment of municipal biosolids is continuously growing. The present studies evaluated the potency of Trametes hirsuta for the reduction in biosolid mass, production of extracellular enzymes, and removal of pharmaceutical compounds (PhACs) in biosolid slurry...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Fungal Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ffunb.2022.896043/full |
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| author | Sabrina Saibi Lounès Haroune Olivier Savary Jean-Philippe Bellenger Hubert Cabana |
| author_facet | Sabrina Saibi Lounès Haroune Olivier Savary Jean-Philippe Bellenger Hubert Cabana |
| author_sort | Sabrina Saibi |
| collection | DOAJ |
| description | The potential of microorganisms for the treatment of municipal biosolids is continuously growing. The present studies evaluated the potency of Trametes hirsuta for the reduction in biosolid mass, production of extracellular enzymes, and removal of pharmaceutical compounds (PhACs) in biosolid slurry in the presence and absence of spiked PhACs [5 non-steroidal anti-inflammatories (NSAIs) and 2 psychoactive compounds (PACs)]. Toxicity after 35 days of fungal treatment was also assessed. Results showed that the growth of T. hirsuta is limited above 25% and wholly inhibited above 50% of biosolids in the slurry. At 12% of biosolid concentration, biosolid mass was reduced by 90%, NSAIs were entirely removed, but PACs' removal was only ~20%. Increasing biosolid content to 25% did not markedly affect biosolid reduction but significantly enhanced the removal of PACs (>50%). Results also showed that both PhACs and biosolids induced the production of oxidative enzymes. In 12% biosolids in the slurry, the oxidative potential measured by the ABTS assay (OABTS) reached 5,000 mM of OABTS in the presence of PhACs, and 2,500 mM of OABTS without PhACs, as compared to 1,200 mM of OABTS in control culture. Finally, we report that white rot fungi (WRF) treatment significantly decreased the toxicity of the biosolids. |
| first_indexed | 2024-12-10T05:08:39Z |
| format | Article |
| id | doaj.art-ad11583af012416f8a1d2a2fcb1e5d69 |
| institution | Directory Open Access Journal |
| issn | 2673-6128 |
| language | English |
| last_indexed | 2024-12-10T05:08:39Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Fungal Biology |
| spelling | doaj.art-ad11583af012416f8a1d2a2fcb1e5d692022-12-22T02:01:09ZengFrontiers Media S.A.Frontiers in Fungal Biology2673-61282022-05-01310.3389/ffunb.2022.896043896043Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsutaSabrina Saibi0Lounès Haroune1Olivier Savary2Jean-Philippe Bellenger3Hubert Cabana4Universitéde Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaSherbrooke Pharmacology Institute, Université de Sherbrooke, Sherbrooke, QC, CanadaUniversitéde Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaDepartment of Chemistry, Université de Sherbrooke, Sherbrooke, QC, CanadaUniversitéde Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaThe potential of microorganisms for the treatment of municipal biosolids is continuously growing. The present studies evaluated the potency of Trametes hirsuta for the reduction in biosolid mass, production of extracellular enzymes, and removal of pharmaceutical compounds (PhACs) in biosolid slurry in the presence and absence of spiked PhACs [5 non-steroidal anti-inflammatories (NSAIs) and 2 psychoactive compounds (PACs)]. Toxicity after 35 days of fungal treatment was also assessed. Results showed that the growth of T. hirsuta is limited above 25% and wholly inhibited above 50% of biosolids in the slurry. At 12% of biosolid concentration, biosolid mass was reduced by 90%, NSAIs were entirely removed, but PACs' removal was only ~20%. Increasing biosolid content to 25% did not markedly affect biosolid reduction but significantly enhanced the removal of PACs (>50%). Results also showed that both PhACs and biosolids induced the production of oxidative enzymes. In 12% biosolids in the slurry, the oxidative potential measured by the ABTS assay (OABTS) reached 5,000 mM of OABTS in the presence of PhACs, and 2,500 mM of OABTS without PhACs, as compared to 1,200 mM of OABTS in control culture. Finally, we report that white rot fungi (WRF) treatment significantly decreased the toxicity of the biosolids.https://www.frontiersin.org/articles/10.3389/ffunb.2022.896043/fullwhite-rot-fungimunicipal biosolidslaccase activitypharmaceutical compounds removaltoxicity |
| spellingShingle | Sabrina Saibi Lounès Haroune Olivier Savary Jean-Philippe Bellenger Hubert Cabana Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta Frontiers in Fungal Biology white-rot-fungi municipal biosolids laccase activity pharmaceutical compounds removal toxicity |
| title | Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta |
| title_full | Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta |
| title_fullStr | Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta |
| title_full_unstemmed | Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta |
| title_short | Impact of Pharmaceutical Compounds in the Bioremediation of Municipal Biosolids by the White-Rot-Fungi Trametes hirsuta |
| title_sort | impact of pharmaceutical compounds in the bioremediation of municipal biosolids by the white rot fungi trametes hirsuta |
| topic | white-rot-fungi municipal biosolids laccase activity pharmaceutical compounds removal toxicity |
| url | https://www.frontiersin.org/articles/10.3389/ffunb.2022.896043/full |
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