Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae
<i>Haemonchus contortus</i> affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. <i>Prosopis laevigata</i> is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/773 |
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| author | Edgar Jesús Delgado-Núñez Alejandro Zamilpa Manasés González-Cortazar Agustín Olmedo-Juárez Alexandre Cardoso-Taketa Ernesto Sánchez-Mendoza Daniel Tapia-Maruri David Osvaldo Salinas-Sánchez Pedro Mendoza-de Gives |
| author_facet | Edgar Jesús Delgado-Núñez Alejandro Zamilpa Manasés González-Cortazar Agustín Olmedo-Juárez Alexandre Cardoso-Taketa Ernesto Sánchez-Mendoza Daniel Tapia-Maruri David Osvaldo Salinas-Sánchez Pedro Mendoza-de Gives |
| author_sort | Edgar Jesús Delgado-Núñez |
| collection | DOAJ |
| description | <i>Haemonchus contortus</i> affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. <i>Prosopis laevigata</i> is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary study where a <i>P. laevigata</i> hydroalcoholic extract (<i>Pl-hae)</i> showed anthelmintic activity (<i>aa</i>) against <i>H. contortus</i>. Searching for bioactive compounds (<i>bac</i>) with high <i>aa</i>, the <i>Pl-hae</i> was fractioned obtaining an aqueous (<i>Aq-F</i>) and an ethyl acetate fraction (<i>EtAc-F</i>), and a flavonoid with <i>aa</i> identified as isorhamnetin was obtained from <i>EtAc-F</i>. Both fractions were in vitro assessed by the egg hatch test (<i>eht</i>) and larval mortality (<i>lm</i>) assays. The <i>bac</i> obtained from <i>EtAc-F</i> were characterised by NMR analysis. The highest <i>aa</i> were recorded with <i>EtAc-F</i>, resulting in 100% <i>eht</i> and 80.45% <i>lm</i> at 0.75 and 30 mg/mL, respectively. Alterations in eggs and larvae attributed to isorhamnetin were recorded by environmental scanning electron microscopy, confocal laser scanning and by high-resolution digital-coupled camera. This flavonoid caused 100% <i>eht</i> at 0.07 mg/mL after 48 h and 100% <i>lm</i> at 7.5 mg/mL after 72 h exposure. Isorhamnetin has promising potential as an anthelmintic against sheep haemonchosis. |
| first_indexed | 2024-03-10T19:47:50Z |
| format | Article |
| id | doaj.art-ad12cbcf002b480f8ab021127c373651 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:47:50Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ad12cbcf002b480f8ab021127c3736512023-11-20T00:39:00ZengMDPI AGBiomolecules2218-273X2020-05-0110577310.3390/biom10050773Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and LarvaeEdgar Jesús Delgado-Núñez0Alejandro Zamilpa1Manasés González-Cortazar2Agustín Olmedo-Juárez3Alexandre Cardoso-Taketa4Ernesto Sánchez-Mendoza5Daniel Tapia-Maruri6David Osvaldo Salinas-Sánchez7Pedro Mendoza-de Gives8Centro de Investigación en Biotecnología, Universidad Autónoma de Morelos, Doctorado en Ciencias Naturales (CEIB-UAEM) Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos CP 62209, MéxicoCentro de Investigación Biomédica del Sur, CIBIS-IMSS, Argentina 1, Col Centro, Xochitepec, Morelos CP 62790, MéxicoCentro de Investigación Biomédica del Sur, CIBIS-IMSS, Argentina 1, Col Centro, Xochitepec, Morelos CP 62790, MéxicoCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso C.P. 62550 Jiutepec, Morelos A.P. 206-CIVAC, MéxicoCentro de Investigación en Biotecnología, Universidad Autónoma de Morelos, Doctorado en Ciencias Naturales (CEIB-UAEM) Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos CP 62209, MéxicoDepartamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, CDMX CP 04960, MéxicoDepartamento de Biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, Calle CEPROBI No. 8, Col. San Isidro, Yautepec, Morelos CP 62731, MéxicoCentro de Investigación en Biodiversidad y Conservación (CIByC-UAEM), Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos CP 62209, MéxicoCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso C.P. 62550 Jiutepec, Morelos A.P. 206-CIVAC, México<i>Haemonchus contortus</i> affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. <i>Prosopis laevigata</i> is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary study where a <i>P. laevigata</i> hydroalcoholic extract (<i>Pl-hae)</i> showed anthelmintic activity (<i>aa</i>) against <i>H. contortus</i>. Searching for bioactive compounds (<i>bac</i>) with high <i>aa</i>, the <i>Pl-hae</i> was fractioned obtaining an aqueous (<i>Aq-F</i>) and an ethyl acetate fraction (<i>EtAc-F</i>), and a flavonoid with <i>aa</i> identified as isorhamnetin was obtained from <i>EtAc-F</i>. Both fractions were in vitro assessed by the egg hatch test (<i>eht</i>) and larval mortality (<i>lm</i>) assays. The <i>bac</i> obtained from <i>EtAc-F</i> were characterised by NMR analysis. The highest <i>aa</i> were recorded with <i>EtAc-F</i>, resulting in 100% <i>eht</i> and 80.45% <i>lm</i> at 0.75 and 30 mg/mL, respectively. Alterations in eggs and larvae attributed to isorhamnetin were recorded by environmental scanning electron microscopy, confocal laser scanning and by high-resolution digital-coupled camera. This flavonoid caused 100% <i>eht</i> at 0.07 mg/mL after 48 h and 100% <i>lm</i> at 7.5 mg/mL after 72 h exposure. Isorhamnetin has promising potential as an anthelmintic against sheep haemonchosis.https://www.mdpi.com/2218-273X/10/5/773<i>Prosopis</i>flavonoidisorhamnetinnematocidal activity<i>Haemonchus</i> |
| spellingShingle | Edgar Jesús Delgado-Núñez Alejandro Zamilpa Manasés González-Cortazar Agustín Olmedo-Juárez Alexandre Cardoso-Taketa Ernesto Sánchez-Mendoza Daniel Tapia-Maruri David Osvaldo Salinas-Sánchez Pedro Mendoza-de Gives Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae Biomolecules <i>Prosopis</i> flavonoid isorhamnetin nematocidal activity <i>Haemonchus</i> |
| title | Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae |
| title_full | Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae |
| title_fullStr | Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae |
| title_full_unstemmed | Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae |
| title_short | Isorhamnetin: A Nematocidal Flavonoid from <i>Prosopis laevigata</i> Leaves Against <i>Haemonchus contortus</i> Eggs and Larvae |
| title_sort | isorhamnetin a nematocidal flavonoid from i prosopis laevigata i leaves against i haemonchus contortus i eggs and larvae |
| topic | <i>Prosopis</i> flavonoid isorhamnetin nematocidal activity <i>Haemonchus</i> |
| url | https://www.mdpi.com/2218-273X/10/5/773 |
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