Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography
The invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasit...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300007 |
| _version_ | 1827879292995895296 |
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| author | Claudia Forero Moisés Wasserman |
| author_facet | Claudia Forero Moisés Wasserman |
| author_sort | Claudia Forero |
| collection | DOAJ |
| description | The invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasite. By immunoblotting and immunoprecipitation with specific antibodies we identified the presence of tropomyosin, myosin, a-actinin, and two different actins in the eluate corresponding to F-actin binding proteins. In addition to these, a 240-260 kDa doublet, different in size from the erythrocyte spectrin, reacted with an antibody against human spectrin. All the above mentioned proteins were metabolically radiolabeled when the parasite was cultured with 35S-methionine. The presence of these proteins in P. falciparum is indicative of a complex cytoskeleton and supports the proposed role for an actin-myosin motor during invasion. |
| first_indexed | 2024-03-12T18:04:42Z |
| format | Article |
| id | doaj.art-ad133bb44e964c968b1436c848638cbd |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:04:42Z |
| publishDate | 2000-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-ad133bb44e964c968b1436c848638cbd2023-08-02T09:32:15ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602000-06-0195332933710.1590/S0074-02762000000300007Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatographyClaudia ForeroMoisés WassermanThe invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasite. By immunoblotting and immunoprecipitation with specific antibodies we identified the presence of tropomyosin, myosin, a-actinin, and two different actins in the eluate corresponding to F-actin binding proteins. In addition to these, a 240-260 kDa doublet, different in size from the erythrocyte spectrin, reacted with an antibody against human spectrin. All the above mentioned proteins were metabolically radiolabeled when the parasite was cultured with 35S-methionine. The presence of these proteins in P. falciparum is indicative of a complex cytoskeleton and supports the proposed role for an actin-myosin motor during invasion.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300007actina-actininmalariamyosinspectrintropomyosinPlasmodium falciparumaffinity chromatography |
| spellingShingle | Claudia Forero Moisés Wasserman Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography Memorias do Instituto Oswaldo Cruz actin a-actinin malaria myosin spectrin tropomyosin Plasmodium falciparum affinity chromatography |
| title | Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography |
| title_full | Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography |
| title_fullStr | Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography |
| title_full_unstemmed | Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography |
| title_short | Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography |
| title_sort | isolation and identification of actin binding proteins in plasmodium falciparum by affinity chromatography |
| topic | actin a-actinin malaria myosin spectrin tropomyosin Plasmodium falciparum affinity chromatography |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300007 |
| work_keys_str_mv | AT claudiaforero isolationandidentificationofactinbindingproteinsinplasmodiumfalciparumbyaffinitychromatography AT moiseswasserman isolationandidentificationofactinbindingproteinsinplasmodiumfalciparumbyaffinitychromatography |