DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS
Simultaneous quantification method of assaying main CYP isoforms substrates-markers and their metabolites in urine using HPLC-MS/MS was developed to determine activity of following isoforms: caffeine/paraxanthine for CYP1A2, losartane/E-3174 for CYP2C9, cortisole/6-β-hydroxycortisole for CYP3A4 and...
| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Russian |
| Publicado em: |
LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2019-01-01
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| Colecção: | Разработка и регистрация лекарственных средств |
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| Acesso em linha: | https://www.pharmjournal.ru/jour/article/view/175 |
| _version_ | 1826569816078548992 |
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| author | E. A. Egorenkov V. V. Smirnov |
| author_facet | E. A. Egorenkov V. V. Smirnov |
| author_sort | E. A. Egorenkov |
| collection | DOAJ |
| description | Simultaneous quantification method of assaying main CYP isoforms substrates-markers and their metabolites in urine using HPLC-MS/MS was developed to determine activity of following isoforms: caffeine/paraxanthine for CYP1A2, losartane/E-3174 for CYP2C9, cortisole/6-β-hydroxycortisole for CYP3A4 and pinoline/6-hydroxy-1,2,3,4-tetrahydro-β-carboline for CYP2D6. Carbamazepine was used as internal standart. Urine was used as biological object, samples were prepared by extraction with ethyl acetate : hexane : diethyl ether (50:35:15) mixture continued with pH correction of urine sample using borate buffer (pH=9,4) and following extraction with ethyl acetate : hexane mixture (50:50). Method was successfully validated and can be put in clinical practice for CYP isoforms activity determination. |
| first_indexed | 2024-04-10T01:41:37Z |
| format | Article |
| id | doaj.art-ad1a953f699f416ea29d5d4b7f5bb00c |
| institution | Directory Open Access Journal |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| last_indexed | 2025-03-14T11:45:20Z |
| publishDate | 2019-01-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj.art-ad1a953f699f416ea29d5d4b7f5bb00c2025-03-02T09:53:18ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492019-01-0104198202175DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MSE. A. Egorenkov0V. V. Smirnov1I.M. Sechenov First Moscow State Medical University; National Research Center Institute of Immunology Federal Medical Biological Agency of RussiaI.M. Sechenov First Moscow State Medical University; National Research Center Institute of Immunology Federal Medical Biological Agency of RussiaSimultaneous quantification method of assaying main CYP isoforms substrates-markers and their metabolites in urine using HPLC-MS/MS was developed to determine activity of following isoforms: caffeine/paraxanthine for CYP1A2, losartane/E-3174 for CYP2C9, cortisole/6-β-hydroxycortisole for CYP3A4 and pinoline/6-hydroxy-1,2,3,4-tetrahydro-β-carboline for CYP2D6. Carbamazepine was used as internal standart. Urine was used as biological object, samples were prepared by extraction with ethyl acetate : hexane : diethyl ether (50:35:15) mixture continued with pH correction of urine sample using borate buffer (pH=9,4) and following extraction with ethyl acetate : hexane mixture (50:50). Method was successfully validated and can be put in clinical practice for CYP isoforms activity determination.https://www.pharmjournal.ru/jour/article/view/175cytochrome p450cyp1a2cyp2c9cyp3a4cyp2d6«cocktail» methodphenotypingcaffeinelosartanecortisolepinoline |
| spellingShingle | E. A. Egorenkov V. V. Smirnov DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS Разработка и регистрация лекарственных средств cytochrome p450 cyp1a2 cyp2c9 cyp3a4 cyp2d6 «cocktail» method phenotyping caffeine losartane cortisole pinoline |
| title | DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS |
| title_full | DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS |
| title_fullStr | DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS |
| title_short | DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ASSAY OF SUBSTRATES-MARKERS OF MAIN CYTOCHROME P450 ISOFORMS AND THEIR METABOLITES USING HPLC-MS/MS |
| title_sort | development and validation of simultaneous assay of substrates markers of main cytochrome p450 isoforms and their metabolites using hplc ms ms |
| topic | cytochrome p450 cyp1a2 cyp2c9 cyp3a4 cyp2d6 «cocktail» method phenotyping caffeine losartane cortisole pinoline |
| url | https://www.pharmjournal.ru/jour/article/view/175 |
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