Analysis of reactive astrogliosis in mouse brain using in situ hybridization combined with immunohistochemistry

Analysis of reactive astrogliosis in mouse brain using in situ hybridization combined with immunohistochemistry

Summary: Reactive astrogliosis is characterized by a profound change in astrocyte phenotype in response to all CNS injuries. Here, we present a revised in situ hybridization and immunohistochemistry (IHC) protocol to label the reactive astrocytes in the mouse brain. Several approaches for quantifyin...

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Bibliographic Details
Main Authors: Ranjithmenon Muraleedharan, Diana Nardini, Ronald Raymond Waclaw, Biplab Dasgupta
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cancer
Metabolism
Microscopy
Model organisms
In situ hybridization
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000824

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