Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China
Abstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after sufficient vaccine coverage. The live-attenuated Japanese encephalitis virus (JEV) vaccine SA14–14-2 is believed to have strongly contribute to this decrease. Another vaccine that seems to ha...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01400-3 |
| _version_ | 1818341045417541632 |
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| author | Xiaoyan Zheng Xiaozheng Yu Yan Wang Lance Turtle Min Cui Ran Wang Chenghong Yin |
| author_facet | Xiaoyan Zheng Xiaozheng Yu Yan Wang Lance Turtle Min Cui Ran Wang Chenghong Yin |
| author_sort | Xiaoyan Zheng |
| collection | DOAJ |
| description | Abstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after sufficient vaccine coverage. The live-attenuated Japanese encephalitis virus (JEV) vaccine SA14–14-2 is believed to have strongly contribute to this decrease. Another vaccine that seems to have decreased in importance is an inactivated vaccine based on the JEV P3 strain, which is considered to be modifiable, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by the Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding the premembrane (prM) and envelope (E) proteins of the P3 strain was assessed in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into the vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized into BALB/c mice, and high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes were secreted upon stimulation with JEV antigens. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in the mice. Conclusions The DNA vaccine pV-JP3ME based on the JEV P3 strain in this study can induce specific humoral immune and cytokine responses and provide complete protection against JEV in mice. |
| first_indexed | 2024-12-13T15:52:33Z |
| format | Article |
| id | doaj.art-ad1f8d5d5522402588ac99c380525551 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-13T15:52:33Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-ad1f8d5d5522402588ac99c3805255512022-12-21T23:39:24ZengBMCVirology Journal1743-422X2020-08-011711810.1186/s12985-020-01400-3Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in ChinaXiaoyan Zheng0Xiaozheng Yu1Yan Wang2Lance Turtle3Min Cui4Ran Wang5Chenghong Yin6Beijing Institute of Tropical Medicine, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Gastroenterology, Beijing Children’s Hospital, Capital Medical UniversityOutpatient Department, Beijing Friendship Hospital, Capital Medical UniversityNIHR Health Protection Research Unit for Emerging and Zoonotic Infections, University of LiverpoolState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityBeijing Key Laboratory of Pediatric Respiratory Infection diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Research Unit of Critical Infection in Children, Chinese Academy of Medical Sciences, 2019RU016, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityAbstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after sufficient vaccine coverage. The live-attenuated Japanese encephalitis virus (JEV) vaccine SA14–14-2 is believed to have strongly contribute to this decrease. Another vaccine that seems to have decreased in importance is an inactivated vaccine based on the JEV P3 strain, which is considered to be modifiable, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by the Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding the premembrane (prM) and envelope (E) proteins of the P3 strain was assessed in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into the vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized into BALB/c mice, and high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes were secreted upon stimulation with JEV antigens. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in the mice. Conclusions The DNA vaccine pV-JP3ME based on the JEV P3 strain in this study can induce specific humoral immune and cytokine responses and provide complete protection against JEV in mice.http://link.springer.com/article/10.1186/s12985-020-01400-3Japanese encephalitis virusP3 strainDNA vaccineprM/EProtection |
| spellingShingle | Xiaoyan Zheng Xiaozheng Yu Yan Wang Lance Turtle Min Cui Ran Wang Chenghong Yin Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China Virology Journal Japanese encephalitis virus P3 strain DNA vaccine prM/E Protection |
| title | Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China |
| title_full | Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China |
| title_fullStr | Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China |
| title_full_unstemmed | Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China |
| title_short | Complete protection for mice conferred by a DNA vaccine based on the Japanese encephalitis virus P3 strain used to prepare the inactivated vaccine in China |
| title_sort | complete protection for mice conferred by a dna vaccine based on the japanese encephalitis virus p3 strain used to prepare the inactivated vaccine in china |
| topic | Japanese encephalitis virus P3 strain DNA vaccine prM/E Protection |
| url | http://link.springer.com/article/10.1186/s12985-020-01400-3 |
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