Duck plague virus Us3 regulates the expression of pUL48
ABSTRACT: Duck plague (DP) is one of the contagious diseases caused by Duck plague virus (DPV), which is a serious threat to the development of duck farming. Us3 is a PKA-like protein kinase in alphaherpesvirus, which can regulate the biological functions of many viral proteins, but whether Us3 regu...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124000774 |
| _version_ | 1797256499040354304 |
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| author | Tong Zhou Peilin Ruan Mingshu Wang Anchun Cheng Wei Zhang Bin Tian Qiao Yang Xumin Ou Di Sun Yu He Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xin-Xin Zhao Yanling Yu Ling Zhang Dekang Zhu Shun Chen Mafeng Liu Renyong Jia |
| author_facet | Tong Zhou Peilin Ruan Mingshu Wang Anchun Cheng Wei Zhang Bin Tian Qiao Yang Xumin Ou Di Sun Yu He Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xin-Xin Zhao Yanling Yu Ling Zhang Dekang Zhu Shun Chen Mafeng Liu Renyong Jia |
| author_sort | Tong Zhou |
| collection | DOAJ |
| description | ABSTRACT: Duck plague (DP) is one of the contagious diseases caused by Duck plague virus (DPV), which is a serious threat to the development of duck farming. Us3 is a PKA-like protein kinase in alphaherpesvirus, which can regulate the biological functions of many viral proteins, but whether Us3 regulates pUL48 protein has not been reported. In this paper, Western Blot, qRT-PCR, dual luciferase reporter system and Co-IP were used to investigate the relationship between pUL48 and Us3. The results showed that: 1) pUL48 interacted with Us3 at 138-256aa through its DBD region. 2) Us3 enhanced the protein expression of pUL48 in a dose-dependent manner. 3) Us3 promoted the mRNA level of pUL48 by activating its promoter activity. 4) Us3 inhibited the transcriptional activation function of pUL48. The results can provide scientific data for perfecting and supplementing the function of alpha herpesvirus Us3 and pUL48. |
| first_indexed | 2024-03-08T00:13:20Z |
| format | Article |
| id | doaj.art-ad21a83ad9c24e9ebea7121a74c6f248 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:43Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-ad21a83ad9c24e9ebea7121a74c6f2482024-03-20T06:07:49ZengElsevierPoultry Science0032-57912024-04-011034103498Duck plague virus Us3 regulates the expression of pUL48Tong Zhou0Peilin Ruan1Mingshu Wang2Anchun Cheng3Wei Zhang4Bin Tian5Qiao Yang6Xumin Ou7Di Sun8Yu He9Zhen Wu10Shaqiu Zhang11Juan Huang12Ying Wu13Xin-Xin Zhao14Yanling Yu15Ling Zhang16Dekang Zhu17Shun Chen18Mafeng Liu19Renyong Jia20Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Corresponding author:Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, ChinaABSTRACT: Duck plague (DP) is one of the contagious diseases caused by Duck plague virus (DPV), which is a serious threat to the development of duck farming. Us3 is a PKA-like protein kinase in alphaherpesvirus, which can regulate the biological functions of many viral proteins, but whether Us3 regulates pUL48 protein has not been reported. In this paper, Western Blot, qRT-PCR, dual luciferase reporter system and Co-IP were used to investigate the relationship between pUL48 and Us3. The results showed that: 1) pUL48 interacted with Us3 at 138-256aa through its DBD region. 2) Us3 enhanced the protein expression of pUL48 in a dose-dependent manner. 3) Us3 promoted the mRNA level of pUL48 by activating its promoter activity. 4) Us3 inhibited the transcriptional activation function of pUL48. The results can provide scientific data for perfecting and supplementing the function of alpha herpesvirus Us3 and pUL48.http://www.sciencedirect.com/science/article/pii/S0032579124000774duck plague virusUs3pUL48 |
| spellingShingle | Tong Zhou Peilin Ruan Mingshu Wang Anchun Cheng Wei Zhang Bin Tian Qiao Yang Xumin Ou Di Sun Yu He Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xin-Xin Zhao Yanling Yu Ling Zhang Dekang Zhu Shun Chen Mafeng Liu Renyong Jia Duck plague virus Us3 regulates the expression of pUL48 Poultry Science duck plague virus Us3 pUL48 |
| title | Duck plague virus Us3 regulates the expression of pUL48 |
| title_full | Duck plague virus Us3 regulates the expression of pUL48 |
| title_fullStr | Duck plague virus Us3 regulates the expression of pUL48 |
| title_full_unstemmed | Duck plague virus Us3 regulates the expression of pUL48 |
| title_short | Duck plague virus Us3 regulates the expression of pUL48 |
| title_sort | duck plague virus us3 regulates the expression of pul48 |
| topic | duck plague virus Us3 pUL48 |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124000774 |
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