Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages
Prolactin-releasing Peptide (PrRP) is a neuropeptide whose receptor is GPR10. Recently, the regulatory role of PrRP in the neuroendocrine field has attracted increasing attention. However, the influence of PrRP on macrophages, the critical housekeeper in the neuroendocrine field, has not yet been fu...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4456 |
| _version_ | 1797536472304189440 |
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| author | Yulong Sun Zhuo Zuo Yuanyuan Kuang |
| author_facet | Yulong Sun Zhuo Zuo Yuanyuan Kuang |
| author_sort | Yulong Sun |
| collection | DOAJ |
| description | Prolactin-releasing Peptide (PrRP) is a neuropeptide whose receptor is GPR10. Recently, the regulatory role of PrRP in the neuroendocrine field has attracted increasing attention. However, the influence of PrRP on macrophages, the critical housekeeper in the neuroendocrine field, has not yet been fully elucidated. Here, we investigated the effect of PrRP on the transcriptome of mouse bone marrow-derived macrophages (BMDMs) with RNA sequencing, bioinformatics, and molecular simulation. BMDMs were exposed to PrRP (18 h) and were subjected to RNA sequencing. Differentially expressed genes (DEGs) were acquired, followed by GO, KEGG, and PPI analysis. Eight qPCR-validated DEGs were chosen as hub genes. Next, the three-dimensional structures of the proteins encoded by these hub genes were modeled by Rosetta and Modeller, followed by molecular dynamics simulation by the Gromacs program. Finally, the binding modes between PrRP and hub proteins were investigated with the Rosetta program. PrRP showed no noticeable effect on the morphology of macrophages. A total of 410 DEGs were acquired, and PrRP regulated multiple BMDM-mediated functional pathways. Besides, the possible docking modes between PrRP and hub proteins were investigated. Moreover, GPR10 was expressed on the cell membrane of BMDMs, which increased after PrRP exposure. Collectively, PrRP significantly changed the transcriptome profile of BMDMs, implying that PrRP may be involved in various physiological activities mastered by macrophages. |
| first_indexed | 2024-03-10T12:00:10Z |
| format | Article |
| id | doaj.art-ad21ea8ce6c340dfa6fc3692d1811a0a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:00:10Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ad21ea8ce6c340dfa6fc3692d1811a0a2023-11-21T16:59:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01229445610.3390/ijms22094456Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived MacrophagesYulong Sun0Zhuo Zuo1Yuanyuan Kuang2School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, ChinaProlactin-releasing Peptide (PrRP) is a neuropeptide whose receptor is GPR10. Recently, the regulatory role of PrRP in the neuroendocrine field has attracted increasing attention. However, the influence of PrRP on macrophages, the critical housekeeper in the neuroendocrine field, has not yet been fully elucidated. Here, we investigated the effect of PrRP on the transcriptome of mouse bone marrow-derived macrophages (BMDMs) with RNA sequencing, bioinformatics, and molecular simulation. BMDMs were exposed to PrRP (18 h) and were subjected to RNA sequencing. Differentially expressed genes (DEGs) were acquired, followed by GO, KEGG, and PPI analysis. Eight qPCR-validated DEGs were chosen as hub genes. Next, the three-dimensional structures of the proteins encoded by these hub genes were modeled by Rosetta and Modeller, followed by molecular dynamics simulation by the Gromacs program. Finally, the binding modes between PrRP and hub proteins were investigated with the Rosetta program. PrRP showed no noticeable effect on the morphology of macrophages. A total of 410 DEGs were acquired, and PrRP regulated multiple BMDM-mediated functional pathways. Besides, the possible docking modes between PrRP and hub proteins were investigated. Moreover, GPR10 was expressed on the cell membrane of BMDMs, which increased after PrRP exposure. Collectively, PrRP significantly changed the transcriptome profile of BMDMs, implying that PrRP may be involved in various physiological activities mastered by macrophages.https://www.mdpi.com/1422-0067/22/9/4456prolactin-releasing peptideGPR10bone marrow-derived macrophageRNA sequencingbioinformaticstranscriptomic profiles |
| spellingShingle | Yulong Sun Zhuo Zuo Yuanyuan Kuang Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages International Journal of Molecular Sciences prolactin-releasing peptide GPR10 bone marrow-derived macrophage RNA sequencing bioinformatics transcriptomic profiles |
| title | Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages |
| title_full | Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages |
| title_fullStr | Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages |
| title_full_unstemmed | Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages |
| title_short | Prolactin-Releasing Peptide Differentially Regulates Gene Transcriptomic Profiles in Mouse Bone Marrow-Derived Macrophages |
| title_sort | prolactin releasing peptide differentially regulates gene transcriptomic profiles in mouse bone marrow derived macrophages |
| topic | prolactin-releasing peptide GPR10 bone marrow-derived macrophage RNA sequencing bioinformatics transcriptomic profiles |
| url | https://www.mdpi.com/1422-0067/22/9/4456 |
| work_keys_str_mv | AT yulongsun prolactinreleasingpeptidedifferentiallyregulatesgenetranscriptomicprofilesinmousebonemarrowderivedmacrophages AT zhuozuo prolactinreleasingpeptidedifferentiallyregulatesgenetranscriptomicprofilesinmousebonemarrowderivedmacrophages AT yuanyuankuang prolactinreleasingpeptidedifferentiallyregulatesgenetranscriptomicprofilesinmousebonemarrowderivedmacrophages |