Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination
Following a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, fol...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580816302631 |
| _version_ | 1828422849983938560 |
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| author | Eleni Mente Graham J. Pierce Efthimia Antonopoulou David Stead Samuel A.M. Martin |
| author_facet | Eleni Mente Graham J. Pierce Efthimia Antonopoulou David Stead Samuel A.M. Martin |
| author_sort | Eleni Mente |
| collection | DOAJ |
| description | Following a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, following a single meal to define the postprandial expression of hepatic proteins. The fish were fed a high marine fishmeal/fish oil single meal following a period of 24 h without feeding. Liver protein profiles were examined by 2D gel electrophoresis just before feeding (time 0 h) and at 6 and 12 h after feeding. Of a total of 588 protein spots analysed in a temporal fashion, 49 differed significantly in abundance between the three time groups (ANOVA, p<0.05), before and after feeding, 15 were increased and 34 were decreased in abundance after feeding. Amino acid metabolism-regulated proteins such as phenylalanine-4-hydroxylase and proliferating cell antigen were increased in abundance 12 and 6 h following the meal, suggesting by this time that the fish were increasing their protein turnover to utilize efficiently their dietary protein consumption. Overall, these results highlight some specificity of the trout metabolism and identify postprandial response of metabolism-related proteins 6–12 h after feeding a single meal. |
| first_indexed | 2024-12-10T15:53:38Z |
| format | Article |
| id | doaj.art-ad220913ecd4471b8b2d19c4476354ce |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-10T15:53:38Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-ad220913ecd4471b8b2d19c4476354ce2022-12-22T01:42:44ZengElsevierBiochemistry and Biophysics Reports2405-58082017-03-019C798510.1016/j.bbrep.2016.10.012Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examinationEleni Mente0Graham J. Pierce1Efthimia Antonopoulou2David Stead3Samuel A.M. Martin4Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Volos, GreeceInstitute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UKLaboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceAberdeen Proteomics, University of Aberdeen, Institute of Medical Sciences, Aberdeen, UKInstitute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UKFollowing a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, following a single meal to define the postprandial expression of hepatic proteins. The fish were fed a high marine fishmeal/fish oil single meal following a period of 24 h without feeding. Liver protein profiles were examined by 2D gel electrophoresis just before feeding (time 0 h) and at 6 and 12 h after feeding. Of a total of 588 protein spots analysed in a temporal fashion, 49 differed significantly in abundance between the three time groups (ANOVA, p<0.05), before and after feeding, 15 were increased and 34 were decreased in abundance after feeding. Amino acid metabolism-regulated proteins such as phenylalanine-4-hydroxylase and proliferating cell antigen were increased in abundance 12 and 6 h following the meal, suggesting by this time that the fish were increasing their protein turnover to utilize efficiently their dietary protein consumption. Overall, these results highlight some specificity of the trout metabolism and identify postprandial response of metabolism-related proteins 6–12 h after feeding a single meal.http://www.sciencedirect.com/science/article/pii/S2405580816302631PostprandialHepatic proteinsTroutProteomicsDietMealNutrition |
| spellingShingle | Eleni Mente Graham J. Pierce Efthimia Antonopoulou David Stead Samuel A.M. Martin Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination Biochemistry and Biophysics Reports Postprandial Hepatic proteins Trout Proteomics Diet Meal Nutrition |
| title | Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination |
| title_full | Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination |
| title_fullStr | Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination |
| title_full_unstemmed | Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination |
| title_short | Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination |
| title_sort | postprandial hepatic protein expression in trout oncorhynchus mykiss a proteomics examination |
| topic | Postprandial Hepatic proteins Trout Proteomics Diet Meal Nutrition |
| url | http://www.sciencedirect.com/science/article/pii/S2405580816302631 |
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