Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model
Background and purpose: Cancer is one of the causes of mortality worldwide. Evidence suggests that iron depletion by a chelator agent suppresses the development of some cancer cells. Deferasirox is shown to have anticancer properties. This research aims to synthesize and evaluate the effects of defe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2022-11-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-18496-en.html |
| _version_ | 1797893167605874688 |
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| author | Hamidreza Mohammadi Javad Akhtari Sara Assadpour Mitra Kamali Pedram Ebrahimnejad |
| author_facet | Hamidreza Mohammadi Javad Akhtari Sara Assadpour Mitra Kamali Pedram Ebrahimnejad |
| author_sort | Hamidreza Mohammadi |
| collection | DOAJ |
| description | Background and purpose: Cancer is one of the causes of mortality worldwide. Evidence suggests that iron depletion by a chelator agent suppresses the development of some cancer cells. Deferasirox is shown to have anticancer properties. This research aims to synthesize and evaluate the effects of deferasirox nanoparticles on human breast cancer cells (SKBR3) and mouse breast cancer cells (4T1) compared with the drug form.
Materials and methods: Deferasirox nanoparticles were synthesized by Poly lactic-co-glycolic acid (PLGA) moiety with emulsion formation and solvent evaporation. Cytotoxicity was evaluated using the MTT assay and their 50% inhibitory concentration (IC50) were assessed in SKBR3 and 4T1.
Results: Findings showed that IC50 of nano-deferasirox for SKBR3 and 4T1 were 9.09±0.011 µg/ml and 6.6±0.032 µg/ml, respectively and the IC50 of the deferasirox drug form for SKBR3 and 4T1 IC50 were 21.07±0.003 µg/ml and 11.08±0.013 µg/ml, respectively.
Conclusion: The anticancer effects of deferasirox were confirmed on the cell lines, and the improved properties and efficacy were also confirmed on two cancer cell lines. Nanoparticle delivery systems improve absorption and drug properties and increase the effectiveness of the drug. |
| first_indexed | 2024-04-10T06:48:36Z |
| format | Article |
| id | doaj.art-ad228c43bb4e4385a12ec34e8087085b |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T06:48:36Z |
| publishDate | 2022-11-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-ad228c43bb4e4385a12ec34e8087085b2023-02-28T09:09:01ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792022-11-01322146576Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell ModelHamidreza Mohammadi0Javad Akhtari1Sara Assadpour2Mitra Kamali3Pedram Ebrahimnejad4 Associate Professor, Department of Toxicology and Pharmacology, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Immunogenetics Research Center, Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran PhD in Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran Pharmacy Student, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Department of Pharmaceutics, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Cancer is one of the causes of mortality worldwide. Evidence suggests that iron depletion by a chelator agent suppresses the development of some cancer cells. Deferasirox is shown to have anticancer properties. This research aims to synthesize and evaluate the effects of deferasirox nanoparticles on human breast cancer cells (SKBR3) and mouse breast cancer cells (4T1) compared with the drug form. Materials and methods: Deferasirox nanoparticles were synthesized by Poly lactic-co-glycolic acid (PLGA) moiety with emulsion formation and solvent evaporation. Cytotoxicity was evaluated using the MTT assay and their 50% inhibitory concentration (IC50) were assessed in SKBR3 and 4T1. Results: Findings showed that IC50 of nano-deferasirox for SKBR3 and 4T1 were 9.09±0.011 µg/ml and 6.6±0.032 µg/ml, respectively and the IC50 of the deferasirox drug form for SKBR3 and 4T1 IC50 were 21.07±0.003 µg/ml and 11.08±0.013 µg/ml, respectively. Conclusion: The anticancer effects of deferasirox were confirmed on the cell lines, and the improved properties and efficacy were also confirmed on two cancer cell lines. Nanoparticle delivery systems improve absorption and drug properties and increase the effectiveness of the drug.http://jmums.mazums.ac.ir/article-1-18496-en.htmlcancer celldeferasirox nanoparticlescytotoxicitymtt |
| spellingShingle | Hamidreza Mohammadi Javad Akhtari Sara Assadpour Mitra Kamali Pedram Ebrahimnejad Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model Journal of Mazandaran University of Medical Sciences cancer cell deferasirox nanoparticles cytotoxicity mtt |
| title | Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model |
| title_full | Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model |
| title_fullStr | Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model |
| title_full_unstemmed | Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model |
| title_short | Synthesis and Characterization of Deferasirox PLGA Nanoparticles and Investigation of Toxicity in Cell Model |
| title_sort | synthesis and characterization of deferasirox plga nanoparticles and investigation of toxicity in cell model |
| topic | cancer cell deferasirox nanoparticles cytotoxicity mtt |
| url | http://jmums.mazums.ac.ir/article-1-18496-en.html |
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