Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran

"nBackground: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoono­tic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, so...

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Main Authors: E Razmjou, S Khademvatan, M Mohebali, S Maraghi, L Akhlaghi, H Oormazdi, AR Meamar, J Saki
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2010-02-01
Series:Iranian Journal of Parasitology
Subjects:
Online Access:http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15218.pdf&manuscript_id=15218
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author E Razmjou
S Khademvatan
M Mohebali
S Maraghi
L Akhlaghi
H Oormazdi
AR Meamar
J Saki
author_facet E Razmjou
S Khademvatan
M Mohebali
S Maraghi
L Akhlaghi
H Oormazdi
AR Meamar
J Saki
author_sort E Razmjou
collection DOAJ
description "nBackground: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoono­tic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran."nMethods: From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments."nResults: L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predomi­nantly of L. major species. The alignment of the mini-exon sequencing isolates with re­ported sequencing of L. major and L. tropica revealed 92%-99% identity."nConclusion: Our study showed that mini-exon PCR-RFLP was useful method to identify the causa­tive species of cutaneous leishmaniasis.
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spelling doaj.art-ad247310778143f6832c67d4faabcdc12022-12-21T17:23:11ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2010-02-01512534Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest IranE RazmjouS KhademvatanM MohebaliS MaraghiL AkhlaghiH OormazdiAR MeamarJ Saki"nBackground: Leishmaniasis is a protozoan disease cause by Leishmania genus. Anthroponotic and zoono­tic cutaneous leishmaniasis are endemic in Iran. The aim of this study was to identify the causative agent of cutaneous leishmaniasis by mini-exon gene in five regions of Khuzestan Province, southwest of Iran."nMethods: From 2007 to 2008 in this cross-sectional study, cutaneous samples were collected from patients referred to Health Centers and Hospitals of the Khuzestan Province for cutaneous leishmaniasis diagnosis and cultured in Novy-MacNeal-Nicolle (NNN) and RPMI 1640. The propagated promastigotes were harvested and Leishmania species of cutaneous leishmaniasis were identified by RFLP and DNA sequencing of the PCR generated fragments."nResults: L. major and L. tropica were the causative agents of cutaneous leishmaniasis by predomi­nantly of L. major species. The alignment of the mini-exon sequencing isolates with re­ported sequencing of L. major and L. tropica revealed 92%-99% identity."nConclusion: Our study showed that mini-exon PCR-RFLP was useful method to identify the causa­tive species of cutaneous leishmaniasis.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15218.pdf&manuscript_id=15218 Cutaneous leishmaniasisMmini-exonRFLPSequencingIran
spellingShingle E Razmjou
S Khademvatan
M Mohebali
S Maraghi
L Akhlaghi
H Oormazdi
AR Meamar
J Saki
Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
Iranian Journal of Parasitology
Cutaneous leishmaniasis
Mmini-exon
RFLP
Sequencing
Iran
title Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
title_full Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
title_fullStr Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
title_full_unstemmed Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
title_short Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
title_sort mini exon genotyping of leishmania species in khuzestan province southwest iran
topic Cutaneous leishmaniasis
Mmini-exon
RFLP
Sequencing
Iran
url http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15218.pdf&manuscript_id=15218
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