Evaluation of gene expression of 14-3-3 genes under drought stress in potato
ObjectivePotato (Solanum tuberosum) is an important food and economic crop in the world. However, potato shows susceptibility to drought stress. Thus, the investigation of the molecular mechanism of drought stress tolerance is essential considered. GF14/14-3-3 protein is one of the conserved dimeric...
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2022-09-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_3436_8d9103aafe2228ae9ccd0225f00e25b4.pdf |
| _version_ | 1797389030123372544 |
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| author | Zahra Hajibarat Abbas Saidi |
| author_facet | Zahra Hajibarat Abbas Saidi |
| author_sort | Zahra Hajibarat |
| collection | DOAJ |
| description | ObjectivePotato (Solanum tuberosum) is an important food and economic crop in the world. However, potato shows susceptibility to drought stress. Thus, the investigation of the molecular mechanism of drought stress tolerance is essential considered. GF14/14-3-3 protein is one of the conserved dimeric proteins that regulate several cellular processes, ranging from metabolism to transport, growth, development, and stress response. However, only a few reports are available regarding the effect of 14-3-3 genes in response to stress in potatoes. Materials and methodsIn this study, twelve 14-3-3 genes were detected in the potato genome using bioinformatics methods. Further, motif analysis, gene structure, phylogenetic analysis, TFBS, and synteny analysis were performed on the 14-3-3 genes. In addition, the expression analysis of two genes (StGF14i and StGF14h) in four tissues (root, stem, leaf, and tuber) and their expression under drought stress in the greenhouse was investigated. ResultsBased on phylogenetic relationships, the StGF14 family members were categorized into two classes. Analysis of transcription factor binding sites (TFBS) in the promoter region of 14-3-3 genes revealed that the highest and the lowest number of TFBs were MYB and CSD, respectively, were found in the promoters of 14-3-3 genes. Moreover, different frequencies of TFSB in 14-3-3 genes could indicate that these genes control different developmental stages and are involved in complex regulatory mechanisms. Furthermore, the genome evolution of S. tuberosum using orthologs and paralogues identification was studied. The number of exons in 14-3-3 genes was from four to seven and most of these genes in the same subfamily had the same exon-intron patterns. The expression of two genes in leaves and tuber under drought stress as well as the gene expression of both genes in root, stem, leaf, and tuber tissues under drought were examined. Based on the expression analysis of two genes StGF14i and StGF14h in tissues and a survey of drought stress, the StGF14i gene has the maximum expression in four tissues and also, the highest expression in tubers under drought stress. Our results revealed that two orthologous gene pairs between S. tuberosum and A. thaliana as well as eight paralogous genes among potato genomes were identified. ConclusionsThe expression patterns of StGF14i and StGF14h genes in different tissues and in response to drought stress that two genes had the conserved and necessary roles in potato growth and development potato. It is hoped that the results of this study will be useful for further investigation of the functional role and molecular mechanisms of 14-3-3 genes in response to drought stresses. |
| first_indexed | 2024-03-08T22:49:53Z |
| format | Article |
| id | doaj.art-ad26515c14a646bda483359cf07b1251 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-03-08T22:49:53Z |
| publishDate | 2022-09-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-ad26515c14a646bda483359cf07b12512023-12-16T17:41:55ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002022-09-01143638210.22103/jab.2022.18681.13603436Evaluation of gene expression of 14-3-3 genes under drought stress in potatoZahra Hajibarat0Abbas Saidi1Ph.D. Student, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.Professor, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.ObjectivePotato (Solanum tuberosum) is an important food and economic crop in the world. However, potato shows susceptibility to drought stress. Thus, the investigation of the molecular mechanism of drought stress tolerance is essential considered. GF14/14-3-3 protein is one of the conserved dimeric proteins that regulate several cellular processes, ranging from metabolism to transport, growth, development, and stress response. However, only a few reports are available regarding the effect of 14-3-3 genes in response to stress in potatoes. Materials and methodsIn this study, twelve 14-3-3 genes were detected in the potato genome using bioinformatics methods. Further, motif analysis, gene structure, phylogenetic analysis, TFBS, and synteny analysis were performed on the 14-3-3 genes. In addition, the expression analysis of two genes (StGF14i and StGF14h) in four tissues (root, stem, leaf, and tuber) and their expression under drought stress in the greenhouse was investigated. ResultsBased on phylogenetic relationships, the StGF14 family members were categorized into two classes. Analysis of transcription factor binding sites (TFBS) in the promoter region of 14-3-3 genes revealed that the highest and the lowest number of TFBs were MYB and CSD, respectively, were found in the promoters of 14-3-3 genes. Moreover, different frequencies of TFSB in 14-3-3 genes could indicate that these genes control different developmental stages and are involved in complex regulatory mechanisms. Furthermore, the genome evolution of S. tuberosum using orthologs and paralogues identification was studied. The number of exons in 14-3-3 genes was from four to seven and most of these genes in the same subfamily had the same exon-intron patterns. The expression of two genes in leaves and tuber under drought stress as well as the gene expression of both genes in root, stem, leaf, and tuber tissues under drought were examined. Based on the expression analysis of two genes StGF14i and StGF14h in tissues and a survey of drought stress, the StGF14i gene has the maximum expression in four tissues and also, the highest expression in tubers under drought stress. Our results revealed that two orthologous gene pairs between S. tuberosum and A. thaliana as well as eight paralogous genes among potato genomes were identified. ConclusionsThe expression patterns of StGF14i and StGF14h genes in different tissues and in response to drought stress that two genes had the conserved and necessary roles in potato growth and development potato. It is hoped that the results of this study will be useful for further investigation of the functional role and molecular mechanisms of 14-3-3 genes in response to drought stresses.https://jab.uk.ac.ir/article_3436_8d9103aafe2228ae9ccd0225f00e25b4.pdfdroughtpromotertfbsphylogeneticparalogous |
| spellingShingle | Zahra Hajibarat Abbas Saidi Evaluation of gene expression of 14-3-3 genes under drought stress in potato مجله بیوتکنولوژی کشاورزی drought promoter tfbs phylogenetic paralogous |
| title | Evaluation of gene expression of 14-3-3 genes under drought stress in potato |
| title_full | Evaluation of gene expression of 14-3-3 genes under drought stress in potato |
| title_fullStr | Evaluation of gene expression of 14-3-3 genes under drought stress in potato |
| title_full_unstemmed | Evaluation of gene expression of 14-3-3 genes under drought stress in potato |
| title_short | Evaluation of gene expression of 14-3-3 genes under drought stress in potato |
| title_sort | evaluation of gene expression of 14 3 3 genes under drought stress in potato |
| topic | drought promoter tfbs phylogenetic paralogous |
| url | https://jab.uk.ac.ir/article_3436_8d9103aafe2228ae9ccd0225f00e25b4.pdf |
| work_keys_str_mv | AT zahrahajibarat evaluationofgeneexpressionof1433genesunderdroughtstressinpotato AT abbassaidi evaluationofgeneexpressionof1433genesunderdroughtstressinpotato |