Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter
A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Valida...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/22/20/7916 |
| _version_ | 1797470027854643200 |
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| author | Sarah Di Nonno Roland Ulber |
| author_facet | Sarah Di Nonno Roland Ulber |
| author_sort | Sarah Di Nonno |
| collection | DOAJ |
| description | A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from <i>Escherichia coli.</i> |
| first_indexed | 2024-03-09T19:30:57Z |
| format | Article |
| id | doaj.art-ad27ee38f8bd4285924cb57b9d3f3917 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T19:30:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ad27ee38f8bd4285924cb57b9d3f39172023-11-24T02:28:32ZengMDPI AGSensors1424-82202022-10-012220791610.3390/s22207916Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and FluorimeterSarah Di Nonno0Roland Ulber1Chair of Bioprocess Engineering, Technical University of Kaiserslautern, 67663 Kaiserslautern, GermanyChair of Bioprocess Engineering, Technical University of Kaiserslautern, 67663 Kaiserslautern, GermanyA novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from <i>Escherichia coli.</i>https://www.mdpi.com/1424-8220/22/20/7916portable devicesfluorimetryphotometry3-D printingeasy fabricationArduino |
| spellingShingle | Sarah Di Nonno Roland Ulber Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter Sensors portable devices fluorimetry photometry 3-D printing easy fabrication Arduino |
| title | Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter |
| title_full | Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter |
| title_fullStr | Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter |
| title_full_unstemmed | Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter |
| title_short | Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter |
| title_sort | portuino a novel portable low cost arduino based photo and fluorimeter |
| topic | portable devices fluorimetry photometry 3-D printing easy fabrication Arduino |
| url | https://www.mdpi.com/1424-8220/22/20/7916 |
| work_keys_str_mv | AT sarahdinonno portuinoanovelportablelowcostarduinobasedphotoandfluorimeter AT rolandulber portuinoanovelportablelowcostarduinobasedphotoandfluorimeter |