Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt
Objectives: This study was performed to probe the antimicrobial resistance and virulence genes profiling in Pseudomonas aeruginosa recovered from the cases of pericarditis in broiler chickens. Materials and Methods: The samples (n = 250) collected from the cases of pericarditis in broiler chickens...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2020-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=99184 |
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| author | Walid Hamdy Hassan Ahmed Mohamed Kamel Ibrahim Salama Abohamra Sayed Shany Hala Sayed Hassan Salam |
| author_facet | Walid Hamdy Hassan Ahmed Mohamed Kamel Ibrahim Salama Abohamra Sayed Shany Hala Sayed Hassan Salam |
| author_sort | Walid Hamdy Hassan |
| collection | DOAJ |
| description | Objectives: This study was performed to probe the antimicrobial resistance and virulence genes profiling in Pseudomonas aeruginosa recovered from the cases of pericarditis in broiler chickens.
Materials and Methods: The samples (n = 250) collected from the cases of pericarditis in broiler chickens were bacteriologically examined. Antimicrobial susceptibility was tested by disc diffusion technique. The isolates were genotypically studied for the presence of antimicrobial resistance and virulence gene traits. Finally, the nucleotide sequence of representative resistance gene (mexR gene) and virulence genes (toxA and lasI genes) was analyzed.
Results: P. aeruginosa was isolated from 45 samples (18%). Antimicrobial susceptibility testing revealed multidrug resistance in most of the recovered P. aeruginosa isolates, whereas colistin and imipenem were the furthermost in vitro-sensitive antibiotics. Antimicrobial resistance genes, such as blaCTX, fox, and mexR, were prevalent in 100%, 80%, and 100% of the isolates, respectively. PCR confirmed virulence genes such as toxA, exoY, lasB, and lasI in 100%, 60%, 80%, and 80% of the isolates, respectively. Nucleotide sequence analysis of representative resistance gene (mexR gene) and virulence genes (toxA and lasI genes) revealed a high correlation between P. aeruginosa recovered from pericarditis in broiler chickens in the present study with PAO1 (reference strain) and with other sequences published on the GenBank representing different localities worldwide.
Conclusion: It could be concluded that P. aeruginosa recovered from pericarditis in broiler chick¬ens in the current study is highly virulent bacteria, resisting most of the therapeutic agents which not only bear hazards for poultry industry but also represent a public health concern. [J Adv Vet Anim Res 2020; 7(3.000): 452-463] |
| first_indexed | 2024-04-11T17:34:46Z |
| format | Article |
| id | doaj.art-ad28893accd944fda4c9907599017a60 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-11T17:34:46Z |
| publishDate | 2020-09-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-ad28893accd944fda4c9907599017a602022-12-22T04:11:44ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102020-09-017345246310.5455/javar.2020.g44199184Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in EgyptWalid Hamdy Hassan0Ahmed Mohamed Kamel Ibrahim1Salama Abohamra Sayed Shany2Hala Sayed Hassan Salam3Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt Beni-Suef University Hostel, Beni-Suef University, Beni-Suef, Egypt Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.Objectives: This study was performed to probe the antimicrobial resistance and virulence genes profiling in Pseudomonas aeruginosa recovered from the cases of pericarditis in broiler chickens. Materials and Methods: The samples (n = 250) collected from the cases of pericarditis in broiler chickens were bacteriologically examined. Antimicrobial susceptibility was tested by disc diffusion technique. The isolates were genotypically studied for the presence of antimicrobial resistance and virulence gene traits. Finally, the nucleotide sequence of representative resistance gene (mexR gene) and virulence genes (toxA and lasI genes) was analyzed. Results: P. aeruginosa was isolated from 45 samples (18%). Antimicrobial susceptibility testing revealed multidrug resistance in most of the recovered P. aeruginosa isolates, whereas colistin and imipenem were the furthermost in vitro-sensitive antibiotics. Antimicrobial resistance genes, such as blaCTX, fox, and mexR, were prevalent in 100%, 80%, and 100% of the isolates, respectively. PCR confirmed virulence genes such as toxA, exoY, lasB, and lasI in 100%, 60%, 80%, and 80% of the isolates, respectively. Nucleotide sequence analysis of representative resistance gene (mexR gene) and virulence genes (toxA and lasI genes) revealed a high correlation between P. aeruginosa recovered from pericarditis in broiler chickens in the present study with PAO1 (reference strain) and with other sequences published on the GenBank representing different localities worldwide. Conclusion: It could be concluded that P. aeruginosa recovered from pericarditis in broiler chick¬ens in the current study is highly virulent bacteria, resisting most of the therapeutic agents which not only bear hazards for poultry industry but also represent a public health concern. [J Adv Vet Anim Res 2020; 7(3.000): 452-463]http://www.ejmanager.com/fulltextpdf.php?mno=99184mdr; p. aeruginosa; pcr; resistance; sequence; virulence |
| spellingShingle | Walid Hamdy Hassan Ahmed Mohamed Kamel Ibrahim Salama Abohamra Sayed Shany Hala Sayed Hassan Salam Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt Journal of Advanced Veterinary and Animal Research mdr; p. aeruginosa; pcr; resistance; sequence; virulence |
| title | Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt |
| title_full | Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt |
| title_fullStr | Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt |
| title_full_unstemmed | Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt |
| title_short | Virulence and resistance determinants in Pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in Egypt |
| title_sort | virulence and resistance determinants in pseudomonas aeruginosa isolated from pericarditis in diseased broiler chickens in egypt |
| topic | mdr; p. aeruginosa; pcr; resistance; sequence; virulence |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=99184 |
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