Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims
The fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguine...
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| Format: | Article |
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MDPI AG
2017-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/2/306 |
| _version_ | 1828498035157499904 |
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| author | Tong Zhou Dong-Ping Xu Sheng-Jun Lin Ya Li Jie Zheng Yue Zhou Jiao-Jiao Zhang Hua-Bin Li |
| author_facet | Tong Zhou Dong-Ping Xu Sheng-Jun Lin Ya Li Jie Zheng Yue Zhou Jiao-Jiao Zhang Hua-Bin Li |
| author_sort | Tong Zhou |
| collection | DOAJ |
| description | The fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguineum Sims, and a response surface methodology was used to optimize the conditions of UAE to maximize the extraction efficiency. The influence of five independent extraction parameters (ethanol concentration, solvent/material ratio, extracting time, temperature, and ultrasound power) on the extraction efficiency were investigated using a single factor experiment, and then a central composite rotatable design was used to investigate the interaction of three key parameters. The results showed that the optimal extraction conditions were 42.98% ethanol, 28.29 mL/g solvent/material ratio, 34.29 min extracting time, 60 °C temperature, and 600 W ultrasound power. Under these conditions, the Trolox equivalent antioxidant capacity (TEAC) value of the extracts was 1074.61 ± 32.56 μmol Trolox/g dry weight (DW). Compared with conventional maceration (723.27 ± 11.61 μmol Trolox/g DW) and Soxhlet extraction methods (518.37 ± 23.23 μmol Trolox/g DW), the UAE method improved the extraction efficiency, in a shorter period of time. In addition, epicatechin gallate, epicatechin, rutin, epigallocatechin, protocatechuic acid, chlorogenic acid, and quercetin, were identified and quantified in the fruit extracts of Melastoma sanguineum Sims by UPLC-MS/MS. |
| first_indexed | 2024-12-11T12:59:44Z |
| format | Article |
| id | doaj.art-ad2a21e7f2244017bf4fff8c4d529f52 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T12:59:44Z |
| publishDate | 2017-02-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ad2a21e7f2244017bf4fff8c4d529f522022-12-22T01:06:30ZengMDPI AGMolecules1420-30492017-02-0122230610.3390/molecules22020306molecules22020306Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum SimsTong Zhou0Dong-Ping Xu1Sheng-Jun Lin2Ya Li3Jie Zheng4Yue Zhou5Jiao-Jiao Zhang6Hua-Bin Li7Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaZhongshan Center for Disease Control and Prevention, Zhongshan 528403, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaThe fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguineum Sims, and a response surface methodology was used to optimize the conditions of UAE to maximize the extraction efficiency. The influence of five independent extraction parameters (ethanol concentration, solvent/material ratio, extracting time, temperature, and ultrasound power) on the extraction efficiency were investigated using a single factor experiment, and then a central composite rotatable design was used to investigate the interaction of three key parameters. The results showed that the optimal extraction conditions were 42.98% ethanol, 28.29 mL/g solvent/material ratio, 34.29 min extracting time, 60 °C temperature, and 600 W ultrasound power. Under these conditions, the Trolox equivalent antioxidant capacity (TEAC) value of the extracts was 1074.61 ± 32.56 μmol Trolox/g dry weight (DW). Compared with conventional maceration (723.27 ± 11.61 μmol Trolox/g DW) and Soxhlet extraction methods (518.37 ± 23.23 μmol Trolox/g DW), the UAE method improved the extraction efficiency, in a shorter period of time. In addition, epicatechin gallate, epicatechin, rutin, epigallocatechin, protocatechuic acid, chlorogenic acid, and quercetin, were identified and quantified in the fruit extracts of Melastoma sanguineum Sims by UPLC-MS/MS.http://www.mdpi.com/1420-3049/22/2/306Melastoma sanguineum Simsfruitantioxidantultrasound-assisted extractionresponse surface methodologyphenolic compounds |
| spellingShingle | Tong Zhou Dong-Ping Xu Sheng-Jun Lin Ya Li Jie Zheng Yue Zhou Jiao-Jiao Zhang Hua-Bin Li Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims Molecules Melastoma sanguineum Sims fruit antioxidant ultrasound-assisted extraction response surface methodology phenolic compounds |
| title | Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims |
| title_full | Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims |
| title_fullStr | Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims |
| title_full_unstemmed | Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims |
| title_short | Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims |
| title_sort | ultrasound assisted extraction and identification of natural antioxidants from the fruit of melastoma sanguineum sims |
| topic | Melastoma sanguineum Sims fruit antioxidant ultrasound-assisted extraction response surface methodology phenolic compounds |
| url | http://www.mdpi.com/1420-3049/22/2/306 |
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