PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT
Although some vitamins have been shown to prevent glucocorticoids induced osteoporosis in short time, the magnitude of this effect remains to be clarified. The aim of this study was to evaluate protective effect of vitamin D3 on methylprednisolone acetate (MPA) induced osteoporosis in rats. Twenty-f...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2007-02-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/3232 |
| _version_ | 1819030177691205632 |
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| author | I. Ragerdi-Kashani A. Sobhani F. Moradi P. Pasbakhsh F. Sargolzaei-Avval |
| author_facet | I. Ragerdi-Kashani A. Sobhani F. Moradi P. Pasbakhsh F. Sargolzaei-Avval |
| author_sort | I. Ragerdi-Kashani |
| collection | DOAJ |
| description | Although some vitamins have been shown to prevent glucocorticoids induced osteoporosis in short time, the magnitude of this effect remains to be clarified. The aim of this study was to evaluate protective effect of vitamin D3 on methylprednisolone acetate (MPA) induced osteoporosis in rats. Twenty-four male Sprague Dawley rats were randomly divided into four groups: Group A (n = 6), was a base line control or normal animals. Group B (n = 6), was treated only normal saline, group C (n = 6), was treated MPA (0.2 mg/kg) subcutaneously for 4 weeks (3 times per a week) and finally group D (n = 6) were administered MPA resemble to group C and treated by Vitamin D3 (0.1 µg/kg dissolved in ethanol daily). Level of calcium, osteocalcin and acid phosphatase in serum were measured before and after treatment. Also, bone mineral density (BMD) of lumber vertebrae was measured by dual energy X-ray absorptiometry. The results showed that the serum calcium level unaffected by MPA in all groups before and after treatment, but the serum osteocalcin level and bone mineral density of lumbar vertebrae were significantly (P < 0.05) decreased in group C compared with groups A and B. In group D serum osteocalcin level increased again significantly (P < 0.05) but increasing of BMD and bone mineral content were not significant. The findings indicate that by using of vitamin D3 in MPA treated rats could increase bone formation and decrease bone resorption. |
| first_indexed | 2024-12-21T06:26:01Z |
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| id | doaj.art-ad2a57f02f024dec905fd449d39fb694 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-21T06:26:01Z |
| publishDate | 2007-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-ad2a57f02f024dec905fd449d39fb6942022-12-21T19:13:08ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942007-02-01451PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT I. Ragerdi-Kashani0 A. Sobhani1 F. Moradi2 P. Pasbakhsh F. Sargolzaei-Avval3 Although some vitamins have been shown to prevent glucocorticoids induced osteoporosis in short time, the magnitude of this effect remains to be clarified. The aim of this study was to evaluate protective effect of vitamin D3 on methylprednisolone acetate (MPA) induced osteoporosis in rats. Twenty-four male Sprague Dawley rats were randomly divided into four groups: Group A (n = 6), was a base line control or normal animals. Group B (n = 6), was treated only normal saline, group C (n = 6), was treated MPA (0.2 mg/kg) subcutaneously for 4 weeks (3 times per a week) and finally group D (n = 6) were administered MPA resemble to group C and treated by Vitamin D3 (0.1 µg/kg dissolved in ethanol daily). Level of calcium, osteocalcin and acid phosphatase in serum were measured before and after treatment. Also, bone mineral density (BMD) of lumber vertebrae was measured by dual energy X-ray absorptiometry. The results showed that the serum calcium level unaffected by MPA in all groups before and after treatment, but the serum osteocalcin level and bone mineral density of lumbar vertebrae were significantly (P < 0.05) decreased in group C compared with groups A and B. In group D serum osteocalcin level increased again significantly (P < 0.05) but increasing of BMD and bone mineral content were not significant. The findings indicate that by using of vitamin D3 in MPA treated rats could increase bone formation and decrease bone resorption.https://acta.tums.ac.ir/index.php/acta/article/view/3232Methylprednisolone acetatevitamin D3bone markers metabolism |
| spellingShingle | I. Ragerdi-Kashani A. Sobhani F. Moradi P. Pasbakhsh F. Sargolzaei-Avval PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT Acta Medica Iranica Methylprednisolone acetate vitamin D3 bone markers metabolism |
| title | PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT |
| title_full | PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT |
| title_fullStr | PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT |
| title_full_unstemmed | PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT |
| title_short | PROTECTIVE EFFECT OF VITAMIN D3 IN METHYLPREDNISOLONE ACETATE (MPA) INDUCED LOSS OF BONE METABOLISM MARKERS AND BONE MINERAL DENSITY IN THE LUMBAR SPINE OF RAT |
| title_sort | protective effect of vitamin d3 in methylprednisolone acetate mpa induced loss of bone metabolism markers and bone mineral density in the lumbar spine of rat |
| topic | Methylprednisolone acetate vitamin D3 bone markers metabolism |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/3232 |
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