Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration
Scaffolds that are used for bone repair should provide an adequate environment for biomineralization by mesenchymal stem cells (MSCs). Recently, decellularized pulp matrices (DPM) have been utilized in endodontics for their high regenerative potential. Inspired by the dystrophic calcification on the...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-12-01
|
| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731420981672 |
| _version_ | 1818726838896164864 |
|---|---|
| author | Dong Joon Lee Patricia Miguez Jane Kwon Renie Daniel Ricardo Padilla Samuel Min Rahim Zalal Ching-Chang Ko Hae Won Shin |
| author_facet | Dong Joon Lee Patricia Miguez Jane Kwon Renie Daniel Ricardo Padilla Samuel Min Rahim Zalal Ching-Chang Ko Hae Won Shin |
| author_sort | Dong Joon Lee |
| collection | DOAJ |
| description | Scaffolds that are used for bone repair should provide an adequate environment for biomineralization by mesenchymal stem cells (MSCs). Recently, decellularized pulp matrices (DPM) have been utilized in endodontics for their high regenerative potential. Inspired by the dystrophic calcification on the pulp matrix known as pulp stone, we developed acellular pulp bioscaffolds and examined their potential in facilitating MSCs mineralization for bone defect repair. Pulp was decellularized, then retention of its structural integrity was confirmed by histological, mechanical, and biochemical evaluations. MSCs were seeded and proliferation, osteogenic gene expression, and biomineralization were assessed to verify DPM’s osteogenic effects in vitro. MicroCT, energy-dispersive X-ray (EDX), and histological analyses were used to confirm that DPM seeded with MSCs result in greater mineralization on rat critical-sized defects than that without MSCs. Overall, our study proves DPM’s potential to serve as a scaffolding material for MSC-mediated bone regeneration for future craniofacial bone tissue engineering. |
| first_indexed | 2024-12-17T22:04:34Z |
| format | Article |
| id | doaj.art-ad2c38ec863d4754af7851c002aa0775 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-17T22:04:34Z |
| publishDate | 2020-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-ad2c38ec863d4754af7851c002aa07752022-12-21T21:30:54ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-12-011110.1177/2041731420981672Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regenerationDong Joon Lee0Patricia Miguez1Jane Kwon2Renie Daniel3Ricardo Padilla4Samuel Min5Rahim Zalal6Ching-Chang Ko7Hae Won Shin8Oral and Craniofacial Health Science Institute, School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Periodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Neurology and Neurosurgery, School of Medicine, University of North Carolina, Chapel Hill, NC, USADepartment of Oral and Maxillofacial Surgery, School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Diagnostic Sciences, School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Science Institute, School of Dentistry, University of North Carolina, Chapel Hill, NC, USAOral and Craniofacial Health Science Institute, School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Orthodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, USADepartment of Neurology and Neurosurgery, School of Medicine, University of North Carolina, Chapel Hill, NC, USAScaffolds that are used for bone repair should provide an adequate environment for biomineralization by mesenchymal stem cells (MSCs). Recently, decellularized pulp matrices (DPM) have been utilized in endodontics for their high regenerative potential. Inspired by the dystrophic calcification on the pulp matrix known as pulp stone, we developed acellular pulp bioscaffolds and examined their potential in facilitating MSCs mineralization for bone defect repair. Pulp was decellularized, then retention of its structural integrity was confirmed by histological, mechanical, and biochemical evaluations. MSCs were seeded and proliferation, osteogenic gene expression, and biomineralization were assessed to verify DPM’s osteogenic effects in vitro. MicroCT, energy-dispersive X-ray (EDX), and histological analyses were used to confirm that DPM seeded with MSCs result in greater mineralization on rat critical-sized defects than that without MSCs. Overall, our study proves DPM’s potential to serve as a scaffolding material for MSC-mediated bone regeneration for future craniofacial bone tissue engineering.https://doi.org/10.1177/2041731420981672 |
| spellingShingle | Dong Joon Lee Patricia Miguez Jane Kwon Renie Daniel Ricardo Padilla Samuel Min Rahim Zalal Ching-Chang Ko Hae Won Shin Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration Journal of Tissue Engineering |
| title | Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| title_full | Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| title_fullStr | Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| title_full_unstemmed | Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| title_short | Decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| title_sort | decellularized pulp matrix as scaffold for mesenchymal stem cell mediated bone regeneration |
| url | https://doi.org/10.1177/2041731420981672 |
| work_keys_str_mv | AT dongjoonlee decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT patriciamiguez decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT janekwon decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT reniedaniel decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT ricardopadilla decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT samuelmin decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT rahimzalal decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT chingchangko decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration AT haewonshin decellularizedpulpmatrixasscaffoldformesenchymalstemcellmediatedboneregeneration |