Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats
Introduction: Diabetes mellitus causes hyperglycemia and associated complications to the brain. In current study, the traditionally reported remedial claims of Agave americana var. marginata has been scientifically investigated in diabetic rats. Methodology: The methanolic extract of leaves of Agave...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016422001505 |
| _version_ | 1828156825292242944 |
|---|---|
| author | Ambreen Aleem Shahla Shahnaz Sana Javaid Waseem Ashraf Muhammad Fawad Rasool Tanveer Ahmad Abdullah F.Alotaibi Khalid S. Albeshri Faleh Alqahtani Imran Imran |
| author_facet | Ambreen Aleem Shahla Shahnaz Sana Javaid Waseem Ashraf Muhammad Fawad Rasool Tanveer Ahmad Abdullah F.Alotaibi Khalid S. Albeshri Faleh Alqahtani Imran Imran |
| author_sort | Ambreen Aleem |
| collection | DOAJ |
| description | Introduction: Diabetes mellitus causes hyperglycemia and associated complications to the brain. In current study, the traditionally reported remedial claims of Agave americana var. marginata has been scientifically investigated in diabetic rats. Methodology: The methanolic extract of leaves of Agave americana var. marginata (Aa.Cr) was characterized for total phenols, flavonoids, and antioxidant potential through in-vitro testing. The rats chronically pre-treated with Aa.Cr (400 and 600 mg/kg) for 45 days were challenged with alloxan-induced hyperglycemia. The dose-dependent effects of Aa.Cr on blood glucose levels and body weights were compared with diabetic rats using glibenclamide (0.6 mg/kg) as a standard. The animals were tested for diabetes-associated neurological comorbidities through behavioral and biochemical evaluation. Results: The phenols and flavonoids enriched Aa.Cr caused a significant dose-dependent hypoglycemic effect. Aa.Cr showed protection from comorbid anxiety, depression and cognitive impairment as compared to diabetic rats. The alanine aminotransferase, total cholesterol, triglycerides and low-density lipoprotein were prominently reduced, and high-density lipoprotein was increased in rats treated with Aa.Cr. Moreover, the oxidative stress in isolated brains was reduced by Aa.Cr. Conclusion: These findings suggest that Aa.Cr is enriched with antioxidant and anti-inflammatory phytoconstituents valuable for diabetes and related neurological complications. |
| first_indexed | 2024-04-11T23:19:18Z |
| format | Article |
| id | doaj.art-ad2ccdd8ff58455abe0d92aee06f90c6 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-11T23:19:18Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-ad2ccdd8ff58455abe0d92aee06f90c62022-12-22T03:57:32ZengElsevierSaudi Pharmaceutical Journal1319-01642022-10-01301013731386Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic ratsAmbreen Aleem0Shahla Shahnaz1Sana Javaid2Waseem Ashraf3Muhammad Fawad Rasool4Tanveer Ahmad5Abdullah F.Alotaibi6Khalid S. Albeshri7Faleh Alqahtani8Imran Imran9Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, Pakistan; Department of Pharmacy, The Women University, Multan, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, PakistanDepartment of Pharmacy Practice, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, PakistanInstitute for Advanced Biosciences (IAB), Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5309, Institut National de la Sante et de la Recherche Medicale U1209, Grenoble Alpes University, 38700 La Tronche, FranceDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, 60800 Multan, Pakistan; Corresponding author.Introduction: Diabetes mellitus causes hyperglycemia and associated complications to the brain. In current study, the traditionally reported remedial claims of Agave americana var. marginata has been scientifically investigated in diabetic rats. Methodology: The methanolic extract of leaves of Agave americana var. marginata (Aa.Cr) was characterized for total phenols, flavonoids, and antioxidant potential through in-vitro testing. The rats chronically pre-treated with Aa.Cr (400 and 600 mg/kg) for 45 days were challenged with alloxan-induced hyperglycemia. The dose-dependent effects of Aa.Cr on blood glucose levels and body weights were compared with diabetic rats using glibenclamide (0.6 mg/kg) as a standard. The animals were tested for diabetes-associated neurological comorbidities through behavioral and biochemical evaluation. Results: The phenols and flavonoids enriched Aa.Cr caused a significant dose-dependent hypoglycemic effect. Aa.Cr showed protection from comorbid anxiety, depression and cognitive impairment as compared to diabetic rats. The alanine aminotransferase, total cholesterol, triglycerides and low-density lipoprotein were prominently reduced, and high-density lipoprotein was increased in rats treated with Aa.Cr. Moreover, the oxidative stress in isolated brains was reduced by Aa.Cr. Conclusion: These findings suggest that Aa.Cr is enriched with antioxidant and anti-inflammatory phytoconstituents valuable for diabetes and related neurological complications.http://www.sciencedirect.com/science/article/pii/S1319016422001505Agave marginateAlloxan-induced diabetesAntioxidantOpen field testMorris water maze testLipid profile |
| spellingShingle | Ambreen Aleem Shahla Shahnaz Sana Javaid Waseem Ashraf Muhammad Fawad Rasool Tanveer Ahmad Abdullah F.Alotaibi Khalid S. Albeshri Faleh Alqahtani Imran Imran Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats Saudi Pharmaceutical Journal Agave marginate Alloxan-induced diabetes Antioxidant Open field test Morris water maze test Lipid profile |
| title | Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats |
| title_full | Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats |
| title_fullStr | Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats |
| title_full_unstemmed | Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats |
| title_short | Chronically administered Agave americana var. marginata extract ameliorates diabetes mellitus, associated behavioral comorbidities and biochemical parameters in alloxan-induced diabetic rats |
| title_sort | chronically administered agave americana var marginata extract ameliorates diabetes mellitus associated behavioral comorbidities and biochemical parameters in alloxan induced diabetic rats |
| topic | Agave marginate Alloxan-induced diabetes Antioxidant Open field test Morris water maze test Lipid profile |
| url | http://www.sciencedirect.com/science/article/pii/S1319016422001505 |
| work_keys_str_mv | AT ambreenaleem chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT shahlashahnaz chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT sanajavaid chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT waseemashraf chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT muhammadfawadrasool chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT tanveerahmad chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT abdullahfalotaibi chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT khalidsalbeshri chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT falehalqahtani chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats AT imranimran chronicallyadministeredagaveamericanavarmarginataextractamelioratesdiabetesmellitusassociatedbehavioralcomorbiditiesandbiochemicalparametersinalloxaninduceddiabeticrats |