Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere
Hollow carbon nitride nanosphere (HCNS) was synthesized via the hard template method to improve the fluorescence characteristics, drug delivery ability, and photocatalytic activity. Blue fluorescent HCNS was utilized as a quenching agent and an internal reference to combine with Cy5-labelled aptamer...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/4/228 |
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| author | Xinyi Liu Jing Xu Yang Lou Chengsi Pan Yin Zhang Zhouping Wang |
| author_facet | Xinyi Liu Jing Xu Yang Lou Chengsi Pan Yin Zhang Zhouping Wang |
| author_sort | Xinyi Liu |
| collection | DOAJ |
| description | Hollow carbon nitride nanosphere (HCNS) was synthesized via the hard template method to improve the fluorescence characteristics, drug delivery ability, and photocatalytic activity. Blue fluorescent HCNS was utilized as a quenching agent and an internal reference to combine with Cy5-labelled aptamer (Cy5-Apt), resulting in an off-on fluorescence aptasensing method for the detection of Salmonella typhimurium (<i>S. typhimurium</i>). Under optimum conditions, this fluorescence assay presented a linear range from 30 to 3 × 10<sup>4</sup> CFU mL<sup>−1</sup> with a detection limit of 13 CFU mL<sup>−1</sup>. In addition, HCNS was also used as a drug carrier to load chloramphenicol (Cap) molecules. The Cap-loading amount of HCNS could reach 550 μg mg<sup>−1</sup> within 24 h, whereas the corresponding Cap-release amount is 302.5 μg mg<sup>−1</sup> under acidic and irradiation conditions. The integration of photocatalyst with antibiotic could endow HCNS-Cap with better disinfection performance. The bactericidal efficiency of HCNS-Cap (95.0%) against <i>S. typhimurium</i> within 12 h was better than those of HCNS (85.1%) and Cap (72.9%). In addition, selective disinfection of <i>S. typhimurium</i> was further realized by decorating aptamer. Within 4 h, almost all <i>S. Typhimurium</i> were inactivated by HCNS-Cap-Apt, whereas only 13.3% and 48.2% of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> cells were killed, respectively. Therefore, HCNS is a promising bio-platform for aptamer-based fluorescence detection and selective disinfection of <i>S. typhimurium</i>. |
| first_indexed | 2024-03-09T11:05:23Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T11:05:23Z |
| publishDate | 2022-04-01 |
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| series | Biosensors |
| spelling | doaj.art-ad2e1638dc7d46fd9726ddf5b16b7fb02023-12-01T00:58:13ZengMDPI AGBiosensors2079-63742022-04-0112422810.3390/bios12040228Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride NanosphereXinyi Liu0Jing Xu1Yang Lou2Chengsi Pan3Yin Zhang4Zhouping Wang5State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, ChinaKey Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaHollow carbon nitride nanosphere (HCNS) was synthesized via the hard template method to improve the fluorescence characteristics, drug delivery ability, and photocatalytic activity. Blue fluorescent HCNS was utilized as a quenching agent and an internal reference to combine with Cy5-labelled aptamer (Cy5-Apt), resulting in an off-on fluorescence aptasensing method for the detection of Salmonella typhimurium (<i>S. typhimurium</i>). Under optimum conditions, this fluorescence assay presented a linear range from 30 to 3 × 10<sup>4</sup> CFU mL<sup>−1</sup> with a detection limit of 13 CFU mL<sup>−1</sup>. In addition, HCNS was also used as a drug carrier to load chloramphenicol (Cap) molecules. The Cap-loading amount of HCNS could reach 550 μg mg<sup>−1</sup> within 24 h, whereas the corresponding Cap-release amount is 302.5 μg mg<sup>−1</sup> under acidic and irradiation conditions. The integration of photocatalyst with antibiotic could endow HCNS-Cap with better disinfection performance. The bactericidal efficiency of HCNS-Cap (95.0%) against <i>S. typhimurium</i> within 12 h was better than those of HCNS (85.1%) and Cap (72.9%). In addition, selective disinfection of <i>S. typhimurium</i> was further realized by decorating aptamer. Within 4 h, almost all <i>S. Typhimurium</i> were inactivated by HCNS-Cap-Apt, whereas only 13.3% and 48.2% of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> cells were killed, respectively. Therefore, HCNS is a promising bio-platform for aptamer-based fluorescence detection and selective disinfection of <i>S. typhimurium</i>.https://www.mdpi.com/2079-6374/12/4/228hollow carbon nitride nanosphereaptamer<i>Salmonella typhimurium</i>fluorescence detectionselective antibacterial |
| spellingShingle | Xinyi Liu Jing Xu Yang Lou Chengsi Pan Yin Zhang Zhouping Wang Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere Biosensors hollow carbon nitride nanosphere aptamer <i>Salmonella typhimurium</i> fluorescence detection selective antibacterial |
| title | Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere |
| title_full | Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere |
| title_fullStr | Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere |
| title_full_unstemmed | Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere |
| title_short | Aptamer-Based Fluorescence Detection and Selective Disinfection of Salmonella Typhimurium by Using Hollow Carbon Nitride Nanosphere |
| title_sort | aptamer based fluorescence detection and selective disinfection of salmonella typhimurium by using hollow carbon nitride nanosphere |
| topic | hollow carbon nitride nanosphere aptamer <i>Salmonella typhimurium</i> fluorescence detection selective antibacterial |
| url | https://www.mdpi.com/2079-6374/12/4/228 |
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