The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation
Fatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the principal components of the membranes, play an activ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/8 |
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| author | Paraskevi Prasinou Ippolito De Amicis Isa Fusaro Roberta Bucci Damiano Cavallini Salvatore Parrillo Maurizio Caputo Alessandro Gramenzi Augusto Carluccio |
| author_facet | Paraskevi Prasinou Ippolito De Amicis Isa Fusaro Roberta Bucci Damiano Cavallini Salvatore Parrillo Maurizio Caputo Alessandro Gramenzi Augusto Carluccio |
| author_sort | Paraskevi Prasinou |
| collection | DOAJ |
| description | Fatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the principal components of the membranes, play an active role in the regulation of the metabolic activities, and their role on spermiogenesis seems to be of great importance for the resulting quality of the sperm and, thus, fertility. With the application of widely used lipidomic techniques, the aim of this study was to evaluate: (a) the fatty acid content of the spermatozoa’s membranes of 26 healthy male Martina Franca donkeys and its possible correlation with sperm parameters, and (b) the evaluation of the composition of the red blood cells’ membrane. PUFA omega-6 are the principal components (40.38%) of the total PUFA content (47.79%) in both types of cells; however, DPA is the predominant one on the spermatozoa’s membrane (27.57%) but is not present in the erythrocyte’s membrane. Spermatozoa’s motility (%) is positively correlated with stearic acid and EPA, and progressive motility (%), with oleic acid. These findings offer information on the composition of both types of cells’ membranes in healthy male MF donkeys and reflect the metabolic transformations of the spermatozoa’s membrane during the maturation period, providing a better perception of the role of fatty acids in sperm parameters and fertility. |
| first_indexed | 2024-03-11T10:13:05Z |
| format | Article |
| id | doaj.art-ad3343d3ffb349efa1064a7a1fd53816 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:13:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ad3343d3ffb349efa1064a7a1fd538162023-11-16T14:17:55ZengMDPI AGAnimals2076-26152022-12-01131810.3390/ani13010008The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary EvaluationParaskevi Prasinou0Ippolito De Amicis1Isa Fusaro2Roberta Bucci3Damiano Cavallini4Salvatore Parrillo5Maurizio Caputo6Alessandro Gramenzi7Augusto Carluccio8Faculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyDepartment of Veterinary Medical Science, University of Bologna, 40064 Bologna, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFaculty of Veterinary Medicine, University of Teramo, Piano d’Accio, 64100 Teramo, ItalyFatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the principal components of the membranes, play an active role in the regulation of the metabolic activities, and their role on spermiogenesis seems to be of great importance for the resulting quality of the sperm and, thus, fertility. With the application of widely used lipidomic techniques, the aim of this study was to evaluate: (a) the fatty acid content of the spermatozoa’s membranes of 26 healthy male Martina Franca donkeys and its possible correlation with sperm parameters, and (b) the evaluation of the composition of the red blood cells’ membrane. PUFA omega-6 are the principal components (40.38%) of the total PUFA content (47.79%) in both types of cells; however, DPA is the predominant one on the spermatozoa’s membrane (27.57%) but is not present in the erythrocyte’s membrane. Spermatozoa’s motility (%) is positively correlated with stearic acid and EPA, and progressive motility (%), with oleic acid. These findings offer information on the composition of both types of cells’ membranes in healthy male MF donkeys and reflect the metabolic transformations of the spermatozoa’s membrane during the maturation period, providing a better perception of the role of fatty acids in sperm parameters and fertility.https://www.mdpi.com/2076-2615/13/1/8donkeyequine lipidomic profilered blood cell membranespermatozoafatty acidsprogressive motility |
| spellingShingle | Paraskevi Prasinou Ippolito De Amicis Isa Fusaro Roberta Bucci Damiano Cavallini Salvatore Parrillo Maurizio Caputo Alessandro Gramenzi Augusto Carluccio The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation Animals donkey equine lipidomic profile red blood cell membrane spermatozoa fatty acids progressive motility |
| title | The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation |
| title_full | The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation |
| title_fullStr | The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation |
| title_full_unstemmed | The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation |
| title_short | The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation |
| title_sort | lipidomics of spermatozoa and red blood cells membrane profile of martina franca donkey preliminary evaluation |
| topic | donkey equine lipidomic profile red blood cell membrane spermatozoa fatty acids progressive motility |
| url | https://www.mdpi.com/2076-2615/13/1/8 |
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