Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats
Background: Apium leptophyllum (Pers.) is an annual herb with traditional appreciation for various pharmacological properties; however, the scientific information on this herb is insufficient. The aim of the present investigation was undertaken to evaluate flavonoidal fraction of A. leptophyllum fru...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | Biomedical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2319417016303080 |
| _version_ | 1817992890935148544 |
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| author | Himanshu Bhusan Sahoo Rakesh Sagar Anjan Kumar Amrita Bhaiji Subrat Kumar Bhattamishra |
| author_facet | Himanshu Bhusan Sahoo Rakesh Sagar Anjan Kumar Amrita Bhaiji Subrat Kumar Bhattamishra |
| author_sort | Himanshu Bhusan Sahoo |
| collection | DOAJ |
| description | Background: Apium leptophyllum (Pers.) is an annual herb with traditional appreciation for various pharmacological properties; however, the scientific information on this herb is insufficient. The aim of the present investigation was undertaken to evaluate flavonoidal fraction of A. leptophyllum fruit (FFALF) against diarrhoea on albino rats.
Methods: The antidiarrhoeal study was conducted by castor oil induce diarrhoea, prostaglandin E2 (PGE2) induced enteropooling and intestinal transit by charcoal meal test. The rats were divided into five groups (six/group). Group I served as control and received orally 2% acacia suspension; Group II served as standard and received orally loperamide (3 mg/kg) or atropine sulphate (5 mg/kg); Group III, IV and V served as test groups and received the FFALF at doses of 5, 10 and 20 mg/kg orally, respectively.
Results: In castor oil-induced diarrhoeal model, the FFALF significantly (p < 0.001) reduced the frequency of diarrhoea, defecation and weight of faeces as well as increased the sodium–potassium ATPase (Na+K+ATPase) activity and decreased nitric oxide (NO) content in the small intestine. In prostaglandin induced enteropooling model, it significantly (p < 0.01) and dose dependently slowed the intestinal fluid accumulation by decreasing the masses and volumes of intestinal fluid where as in charcoal meal test, it decreased charcoal meal transit in gastrointestinal tract as compared with control.
Conclusions: The study reveals that the FFALF possess anti-diarrhoeal properties mediated through inhibition of hyper secretion and gastrointestinal motility which support the traditional use of the plant. |
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| format | Article |
| id | doaj.art-ad340c518a1c4d9b907ef9c400b39c1f |
| institution | Directory Open Access Journal |
| issn | 2319-4170 |
| language | English |
| last_indexed | 2024-04-14T01:32:10Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Journal |
| spelling | doaj.art-ad340c518a1c4d9b907ef9c400b39c1f2022-12-22T02:20:07ZengElsevierBiomedical Journal2319-41702016-12-0139637638110.1016/j.bj.2016.11.003Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in ratsHimanshu Bhusan Sahoo0Rakesh Sagar1Anjan Kumar2Amrita Bhaiji3Subrat Kumar Bhattamishra4Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, RKDF University, IndiaDepartment of Pharmacognosy and Phytochemistry, Sri Satya Sai Institute of Pharmaceutical Sciences, RKDF University, IndiaDepartment of Pharmaceutical Chemistry, Roland Institute of Pharmaceutical Sciences, IndiaDepartment of Pharmaceutical Chemistry, Roland Institute of Pharmaceutical Sciences, IndiaDepartment of Life Sciences, School of Pharmacy, International Medical University, MalaysiaBackground: Apium leptophyllum (Pers.) is an annual herb with traditional appreciation for various pharmacological properties; however, the scientific information on this herb is insufficient. The aim of the present investigation was undertaken to evaluate flavonoidal fraction of A. leptophyllum fruit (FFALF) against diarrhoea on albino rats. Methods: The antidiarrhoeal study was conducted by castor oil induce diarrhoea, prostaglandin E2 (PGE2) induced enteropooling and intestinal transit by charcoal meal test. The rats were divided into five groups (six/group). Group I served as control and received orally 2% acacia suspension; Group II served as standard and received orally loperamide (3 mg/kg) or atropine sulphate (5 mg/kg); Group III, IV and V served as test groups and received the FFALF at doses of 5, 10 and 20 mg/kg orally, respectively. Results: In castor oil-induced diarrhoeal model, the FFALF significantly (p < 0.001) reduced the frequency of diarrhoea, defecation and weight of faeces as well as increased the sodium–potassium ATPase (Na+K+ATPase) activity and decreased nitric oxide (NO) content in the small intestine. In prostaglandin induced enteropooling model, it significantly (p < 0.01) and dose dependently slowed the intestinal fluid accumulation by decreasing the masses and volumes of intestinal fluid where as in charcoal meal test, it decreased charcoal meal transit in gastrointestinal tract as compared with control. Conclusions: The study reveals that the FFALF possess anti-diarrhoeal properties mediated through inhibition of hyper secretion and gastrointestinal motility which support the traditional use of the plant.http://www.sciencedirect.com/science/article/pii/S2319417016303080Apium leptophyllumCastor oilEnteropoolingIntestinal transitNa+K+ATPase |
| spellingShingle | Himanshu Bhusan Sahoo Rakesh Sagar Anjan Kumar Amrita Bhaiji Subrat Kumar Bhattamishra Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats Biomedical Journal Apium leptophyllum Castor oil Enteropooling Intestinal transit Na+K+ATPase |
| title | Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats |
| title_full | Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats |
| title_fullStr | Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats |
| title_full_unstemmed | Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats |
| title_short | Antidiarrhoeal investigation of Apium leptophyllum (Pers.) by modulation of Na+K+ATPase, nitrous oxide and intestinal transit in rats |
| title_sort | antidiarrhoeal investigation of apium leptophyllum pers by modulation of na k atpase nitrous oxide and intestinal transit in rats |
| topic | Apium leptophyllum Castor oil Enteropooling Intestinal transit Na+K+ATPase |
| url | http://www.sciencedirect.com/science/article/pii/S2319417016303080 |
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