Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens.
Patients with a history of non-muscle-invasive bladder cancer sometimes have recurrence of tumors after transurethral resection of bladder tumor treatment. To find factors related to the recurrence of non-muscle-invasive bladder cancer, we examined tissue specimens taken at transurethral resection o...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6160186?pdf=render |
| _version_ | 1819147430352912384 |
|---|---|
| author | Ryosuke Murai Yasushi Itoh Susumu Kageyama Misako Nakayama Hirohito Ishigaki Kazuo Teramoto Mitsuhiro Narita Tetsuya Yoshida Keiji Tomita Ken-Ichi Kobayashi Akinori Wada Masayuki Nagasawa Shigehisa Kubota Kazumasa Ogasawara Akihiro Kawauchi |
| author_facet | Ryosuke Murai Yasushi Itoh Susumu Kageyama Misako Nakayama Hirohito Ishigaki Kazuo Teramoto Mitsuhiro Narita Tetsuya Yoshida Keiji Tomita Ken-Ichi Kobayashi Akinori Wada Masayuki Nagasawa Shigehisa Kubota Kazumasa Ogasawara Akihiro Kawauchi |
| author_sort | Ryosuke Murai |
| collection | DOAJ |
| description | Patients with a history of non-muscle-invasive bladder cancer sometimes have recurrence of tumors after transurethral resection of bladder tumor treatment. To find factors related to the recurrence of non-muscle-invasive bladder cancer, we examined tissue specimens taken at transurethral resection of bladder tumor as an initial treatment. We revealed the association between prognosis of non-muscle-invasive bladder cancer and infiltration of Foxp3+ T cells that suppress anti-tumor immunity in 115 primary non-muscle-invasive bladder cancer patients retrospectively identified and followed for at least 3 months after primary transurethral resection. In immunohistological staining, we counted the number of cells positive for CD3 and positive for CD3 and Foxp3 together and calculated the percentage of Foxp3+ T cells among the CD3+ T cells. The recurrence-free survival rate was calculated by the Kaplan-Meier method, and a Cox regression analysis of recurrence factors was performed. The median (interquartile range) percentage of Foxp3+ T cells in all cases was 17.1% (11.9, 11.4-23.3%). Compared by risk stratification, it was 11.4% (10.4, 7.8-18.2%) in the low-risk group (n = 32), 16.8% (12.6, 11.6-24.2%) in the intermediate-risk group (n = 45), and 22.0% (9.7, 16.4-26.1%) in the high-risk group (n = 38). The Kaplan-Meier survival analysis indicated that the Foxp3+ T cell high group (≥ 17.1%) had a worse RFS rate than did the low group (< 17.1%) (P = 0.006). In multivariate analysis, the percentage of Foxp3+ T cells was an independent risk factor for intravesical recurrence (hazard ratio 2.25). Thus, peritumoral Foxp3+ T cell infiltration was correlated to risk stratification and recurrence-free survival. Therefore, the percentage of Foxp3+ T cells in tumor specimens may predict a risk for intravesical recurrence. |
| first_indexed | 2024-12-22T13:29:42Z |
| format | Article |
| id | doaj.art-ad34490747cf43e2a2f5703da3306fea |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:29:42Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad34490747cf43e2a2f5703da3306fea2022-12-21T18:24:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01139e020474510.1371/journal.pone.0204745Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens.Ryosuke MuraiYasushi ItohSusumu KageyamaMisako NakayamaHirohito IshigakiKazuo TeramotoMitsuhiro NaritaTetsuya YoshidaKeiji TomitaKen-Ichi KobayashiAkinori WadaMasayuki NagasawaShigehisa KubotaKazumasa OgasawaraAkihiro KawauchiPatients with a history of non-muscle-invasive bladder cancer sometimes have recurrence of tumors after transurethral resection of bladder tumor treatment. To find factors related to the recurrence of non-muscle-invasive bladder cancer, we examined tissue specimens taken at transurethral resection of bladder tumor as an initial treatment. We revealed the association between prognosis of non-muscle-invasive bladder cancer and infiltration of Foxp3+ T cells that suppress anti-tumor immunity in 115 primary non-muscle-invasive bladder cancer patients retrospectively identified and followed for at least 3 months after primary transurethral resection. In immunohistological staining, we counted the number of cells positive for CD3 and positive for CD3 and Foxp3 together and calculated the percentage of Foxp3+ T cells among the CD3+ T cells. The recurrence-free survival rate was calculated by the Kaplan-Meier method, and a Cox regression analysis of recurrence factors was performed. The median (interquartile range) percentage of Foxp3+ T cells in all cases was 17.1% (11.9, 11.4-23.3%). Compared by risk stratification, it was 11.4% (10.4, 7.8-18.2%) in the low-risk group (n = 32), 16.8% (12.6, 11.6-24.2%) in the intermediate-risk group (n = 45), and 22.0% (9.7, 16.4-26.1%) in the high-risk group (n = 38). The Kaplan-Meier survival analysis indicated that the Foxp3+ T cell high group (≥ 17.1%) had a worse RFS rate than did the low group (< 17.1%) (P = 0.006). In multivariate analysis, the percentage of Foxp3+ T cells was an independent risk factor for intravesical recurrence (hazard ratio 2.25). Thus, peritumoral Foxp3+ T cell infiltration was correlated to risk stratification and recurrence-free survival. Therefore, the percentage of Foxp3+ T cells in tumor specimens may predict a risk for intravesical recurrence.http://europepmc.org/articles/PMC6160186?pdf=render |
| spellingShingle | Ryosuke Murai Yasushi Itoh Susumu Kageyama Misako Nakayama Hirohito Ishigaki Kazuo Teramoto Mitsuhiro Narita Tetsuya Yoshida Keiji Tomita Ken-Ichi Kobayashi Akinori Wada Masayuki Nagasawa Shigehisa Kubota Kazumasa Ogasawara Akihiro Kawauchi Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. PLoS ONE |
| title | Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. |
| title_full | Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. |
| title_fullStr | Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. |
| title_full_unstemmed | Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. |
| title_short | Prediction of intravesical recurrence of non-muscle-invasive bladder cancer by evaluation of intratumoral Foxp3+ T cells in the primary transurethral resection of bladder tumor specimens. |
| title_sort | prediction of intravesical recurrence of non muscle invasive bladder cancer by evaluation of intratumoral foxp3 t cells in the primary transurethral resection of bladder tumor specimens |
| url | http://europepmc.org/articles/PMC6160186?pdf=render |
| work_keys_str_mv | AT ryosukemurai predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT yasushiitoh predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT susumukageyama predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT misakonakayama predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT hirohitoishigaki predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT kazuoteramoto predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT mitsuhironarita predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT tetsuyayoshida predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT keijitomita predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT kenichikobayashi predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT akinoriwada predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT masayukinagasawa predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT shigehisakubota predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT kazumasaogasawara predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens AT akihirokawauchi predictionofintravesicalrecurrenceofnonmuscleinvasivebladdercancerbyevaluationofintratumoralfoxp3tcellsintheprimarytransurethralresectionofbladdertumorspecimens |