Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio)
Mechanical skin lesions are a persistent issue under aquaculture operations compromising fish health and robustness. Using an established zebrafish model, the study compared the potential skin wound healing benefit of dietary supplementation with a single-strain yeast fraction rich in β-glucans (β-g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002806 |
| _version_ | 1797676639625150464 |
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| author | Shan Lakmal Edirisinghe Chamilani Nikapitiya Cheol Hee Kim Mathieu Castex Eric Leclercq Mahanama De Zoysa |
| author_facet | Shan Lakmal Edirisinghe Chamilani Nikapitiya Cheol Hee Kim Mathieu Castex Eric Leclercq Mahanama De Zoysa |
| author_sort | Shan Lakmal Edirisinghe |
| collection | DOAJ |
| description | Mechanical skin lesions are a persistent issue under aquaculture operations compromising fish health and robustness. Using an established zebrafish model, the study compared the potential skin wound healing benefit of dietary supplementation with a single-strain yeast fraction rich in β-glucans (β-glucan diet) or with a multi-strain yeast fraction (MsYF). Adult zebrafish were divided into four triplicated groups (12 tanks; 25 fish/tank) as negative control (unwounded-basal diet); control (wounded-basal diet), β-glucan (wounded-β-glucan diet), and MsYF (wounded-MsYF diet) and, following 7-week of nutritional preparation, inflicted with a full-thickness skin wound using a biopsy punch. Wound healing was assessed macroscopically by measuring wound surface area (18 fish individually tracked/group) until full healing within 4 weeks; as well as by histopathological diagnostic, transcriptional and immunoblotting analysis at the wound site at 1, 4 and 9 day post-wound (dpw) over the early healing phase (re-epithelization and inflammation). Following an initial expansion, wound closure was first measured at 4 dpw in the β-glucan and MsYF groups showing a positive and significantly higher daily wound closure rate compared to the control. Later at 16 dpw, cumulative and daily wound closure were significantly higher in the MsYF compared to the β-glucan and control groups. Enhanced gross wound healing was confirmed by histopathological diagnosis of mitigated inflammation, earlier re-epithelization and significantly enhanced granulation tissue synthesis in the MsYF group. Targeted transcriptomic and proteomic markers documented an early up-regulation of matrix metalloproteinases and a dampening or quicker resolution of inflammatory and cellular stress markers in the healing tissues of the MsYF compared to the control group while the β-glucan diet overall elicited intermediary results. Overall, this study demonstrates that dietary supplementation with a yeast-based functional compound can modulate fish immune response in wound healing and promote early wound closure indicating a role for skin health management under aquaculture operations. |
| first_indexed | 2024-03-11T22:32:10Z |
| format | Article |
| id | doaj.art-ad3837603c494cc09fb1cf94bf73fa5f |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-11T22:32:10Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ad3837603c494cc09fb1cf94bf73fa5f2023-09-23T05:11:31ZengElsevierAquaculture Reports2352-51342023-10-0132101741Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio)Shan Lakmal Edirisinghe0Chamilani Nikapitiya1Cheol Hee Kim2Mathieu Castex3Eric Leclercq4Mahanama De Zoysa5College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of KoreaCollege of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of KoreaDepartment of Biology, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of KoreaLallemand SAS, 31700 Blagnac, FranceLallemand SAS, 31700 Blagnac, France; Corresponding authors.College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea; Corresponding authors.Mechanical skin lesions are a persistent issue under aquaculture operations compromising fish health and robustness. Using an established zebrafish model, the study compared the potential skin wound healing benefit of dietary supplementation with a single-strain yeast fraction rich in β-glucans (β-glucan diet) or with a multi-strain yeast fraction (MsYF). Adult zebrafish were divided into four triplicated groups (12 tanks; 25 fish/tank) as negative control (unwounded-basal diet); control (wounded-basal diet), β-glucan (wounded-β-glucan diet), and MsYF (wounded-MsYF diet) and, following 7-week of nutritional preparation, inflicted with a full-thickness skin wound using a biopsy punch. Wound healing was assessed macroscopically by measuring wound surface area (18 fish individually tracked/group) until full healing within 4 weeks; as well as by histopathological diagnostic, transcriptional and immunoblotting analysis at the wound site at 1, 4 and 9 day post-wound (dpw) over the early healing phase (re-epithelization and inflammation). Following an initial expansion, wound closure was first measured at 4 dpw in the β-glucan and MsYF groups showing a positive and significantly higher daily wound closure rate compared to the control. Later at 16 dpw, cumulative and daily wound closure were significantly higher in the MsYF compared to the β-glucan and control groups. Enhanced gross wound healing was confirmed by histopathological diagnosis of mitigated inflammation, earlier re-epithelization and significantly enhanced granulation tissue synthesis in the MsYF group. Targeted transcriptomic and proteomic markers documented an early up-regulation of matrix metalloproteinases and a dampening or quicker resolution of inflammatory and cellular stress markers in the healing tissues of the MsYF compared to the control group while the β-glucan diet overall elicited intermediary results. Overall, this study demonstrates that dietary supplementation with a yeast-based functional compound can modulate fish immune response in wound healing and promote early wound closure indicating a role for skin health management under aquaculture operations.http://www.sciencedirect.com/science/article/pii/S2352513423002806Multi-strain yeast fractionFunctional nutritionImmune-modulationWound healingMucosal healthZebrafish |
| spellingShingle | Shan Lakmal Edirisinghe Chamilani Nikapitiya Cheol Hee Kim Mathieu Castex Eric Leclercq Mahanama De Zoysa Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) Aquaculture Reports Multi-strain yeast fraction Functional nutrition Immune-modulation Wound healing Mucosal health Zebrafish |
| title | Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) |
| title_full | Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) |
| title_fullStr | Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) |
| title_full_unstemmed | Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) |
| title_short | Dietary supplementation with a multi-strain yeast fraction enhances skin wound healing in zebrafish (Danio rerio) |
| title_sort | dietary supplementation with a multi strain yeast fraction enhances skin wound healing in zebrafish danio rerio |
| topic | Multi-strain yeast fraction Functional nutrition Immune-modulation Wound healing Mucosal health Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002806 |
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