Mining methods and typical structural mechanisms of terpene cyclases
Abstract Terpenoids, formed by cyclization and/or permutation of isoprenes, are the most diverse and abundant class of natural products with a broad range of significant functions. One family of the critical enzymes involved in terpenoid biosynthesis is terpene cyclases (TCs), also known as terpene...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-07-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-021-00421-2 |
| _version_ | 1818909191368081408 |
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| author | Zheng-Yu Huang Ru-Yi Ye Hui-Lei Yu Ai-Tao Li Jian-He Xu |
| author_facet | Zheng-Yu Huang Ru-Yi Ye Hui-Lei Yu Ai-Tao Li Jian-He Xu |
| author_sort | Zheng-Yu Huang |
| collection | DOAJ |
| description | Abstract Terpenoids, formed by cyclization and/or permutation of isoprenes, are the most diverse and abundant class of natural products with a broad range of significant functions. One family of the critical enzymes involved in terpenoid biosynthesis is terpene cyclases (TCs), also known as terpene synthases (TSs), which are responsible for forming the ring structure as a backbone of functionally diverse terpenoids. With the recent advances in biotechnology, the researches on terpene cyclases have gradually shifted from the genomic mining of novel enzyme resources to the analysis of their structures and mechanisms. In this review, we summarize both the new methods for genomic mining and the structural mechanisms of some typical terpene cyclases, which are helpful for the discovery, engineering and application of more and new TCs. |
| first_indexed | 2024-12-19T22:22:59Z |
| format | Article |
| id | doaj.art-ad39df1639d442afb9e811d5e370731d |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-19T22:22:59Z |
| publishDate | 2021-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-ad39df1639d442afb9e811d5e370731d2022-12-21T20:03:35ZengSpringerOpenBioresources and Bioprocessing2197-43652021-07-018112710.1186/s40643-021-00421-2Mining methods and typical structural mechanisms of terpene cyclasesZheng-Yu Huang0Ru-Yi Ye1Hui-Lei Yu2Ai-Tao Li3Jian-He Xu4State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and TechnologyState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei UniversityState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and TechnologyAbstract Terpenoids, formed by cyclization and/or permutation of isoprenes, are the most diverse and abundant class of natural products with a broad range of significant functions. One family of the critical enzymes involved in terpenoid biosynthesis is terpene cyclases (TCs), also known as terpene synthases (TSs), which are responsible for forming the ring structure as a backbone of functionally diverse terpenoids. With the recent advances in biotechnology, the researches on terpene cyclases have gradually shifted from the genomic mining of novel enzyme resources to the analysis of their structures and mechanisms. In this review, we summarize both the new methods for genomic mining and the structural mechanisms of some typical terpene cyclases, which are helpful for the discovery, engineering and application of more and new TCs.https://doi.org/10.1186/s40643-021-00421-2Terpene cyclasesBiochemical propertiesReviewGenomic miningStructural analysisCatalytic mechanisms |
| spellingShingle | Zheng-Yu Huang Ru-Yi Ye Hui-Lei Yu Ai-Tao Li Jian-He Xu Mining methods and typical structural mechanisms of terpene cyclases Bioresources and Bioprocessing Terpene cyclases Biochemical properties Review Genomic mining Structural analysis Catalytic mechanisms |
| title | Mining methods and typical structural mechanisms of terpene cyclases |
| title_full | Mining methods and typical structural mechanisms of terpene cyclases |
| title_fullStr | Mining methods and typical structural mechanisms of terpene cyclases |
| title_full_unstemmed | Mining methods and typical structural mechanisms of terpene cyclases |
| title_short | Mining methods and typical structural mechanisms of terpene cyclases |
| title_sort | mining methods and typical structural mechanisms of terpene cyclases |
| topic | Terpene cyclases Biochemical properties Review Genomic mining Structural analysis Catalytic mechanisms |
| url | https://doi.org/10.1186/s40643-021-00421-2 |
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